[Morphological and functional aspects of wound regeneration in the treatment by iodine-containing ointments].

The comprehensive morphological investigation indicated a high antimicrobial activity of iodine-containing ointments in the treatment of septic wounds from the first days of the ointments using. 3% ointment "Stellanin-PEG" helps to reduce the local inflammatory changes by activating neutrophils and macrophages. A management of wounds by 3% ointment "Stellanin-PEG" improves their condition by stimulating regeneration, which leads to an intensification of the proliferative and functional activity of granulation tissue. Clinical, morphological and cytological data are evidence of time reduction of relief of purulent process, which leads to an earlier (in comparison with the use of ointment "Betadine") wound epithelization.

[Fine root nitrogen contents and morphological adaptations of alpine plants].

Nitrogen and carbon contents of fine roots were studied for 92 alpine plant species in the Northwest Caucasus. Nitrogen content ranged from 0.43% (Bromus variegatus) to 3.75% (Corydalis conorhiza) with mean value 1.3%. Carbon content ranged from 40.3% (Corydalis conorhiza) to 51.7% (Empetrum nigrum) with mean value 43.4%. C:N ratio was found to be 34:1 which is higher than the worldwide mean. Eudicot's roots had higher N concentration (1.37 +/- 0.07) than monocot's ones (0.95 +/- 0.09). Among the life forms, carbon content increased in the following order: geophytes < hemicriptophytes < chamaephytes. Specific root length positively correlated with nitrogen root content and negatively--with carbon root content. Species with larger leaves and higher specific root area had more nitrogen and less carbon in roots in comparison with species with small leaves. There were positive correlations between leaf and root nitrogen, as well as carbon, contents. Regrowth rate; seed size, aboveground biomass, and vegetation mobility were not related with root nitrogen content. Our results corroborate the poor and rich soil adaptation syndromes. Species of competitive and ruderal (sensu Grime) strategies are more typical for alpine meadows and snow bed communities. They had higher nitrogen contents in leaves and roots, larger leaves with higher water content and higher rate of seed production. On the other hand, stress-tolerant plants had higher carbon and less nitrogen concentrations in their roots and leaves, smaller leaves and specific leaf area.

[Complex ultrasonic diagnosis of large and gigantic non-parasitic cysts of liver].

The results of clinical and complex ultrasonic examination of 95 patients with large and gigantic liver non-parasitic cysts (LNC) are analyzed. The morphological examination was performed in all the cases. The mean diameter of large LNC was 105+/-7 mm, of gigantic cysts -- 149+/-33 mm (p<0,05), cyst volume was respectively 600+/-142 and 1721+/-954 ml (p<0,05). Analysis of clinical, ultrasonic and laboratory data allowed to describe three variants of large and gigantic, complicated and non-complicated cysts with 97,9% accuracy. The 3-D US reconstruction permitted to precise the anatomic structure and trace selection during transcutaneous US-assisted treatment.

[Ultrasound-assisted puncture method of treatment of hepatic hydatid cysts]. / Punktsionnyi metod lecheniia ékhinokokkovykh kist pecheni pod kontrolem ul'trazvukovogo issledovaniia.

Results of treatment of 31 hepatic hydatid cysts in 28 patients were analyzed. The size of the s ranged from 3 to 25 cm. In 26 cases the cysts contained flive es, in 2 cases they were suppurated after US-assisted intraoperative puncture. Simple hydatid cysts (type I by M. Milcevic) were diagnosed in 24 patients (solitary--in 22, multiple--in 2), solitary cysts of type II--in 2 patients. In 23 cases aspiration-catheter treatment with complete removal of chitin membrane by one-stage (4) or two-stage (19) methods was performed. After removal of chitin membrane in 3 patients lateral fistula between spurious cysts cavities and peripheral branches of intrahepatic bile ducts were revealed which spontaneously. Glue composition "Rabrom" was used in 2 cases for closure of the residual cavity. In 4 patients who had cysts less 6 cm in size puncture irrigation of chitin cavity with 30% NaCl (PAIR technique) without it removal was performed. In one patient transcutaneous catheter (PAIR-PD technique) was used for irrigation of the cyst larger than 6 cm. The duration of two-stage aspiration-catheter treatment with removal of chitin membrane was 25.6 +/- 9.6 days. In moderate calcinosis of the fibrous membrane and bile fistulas period of hospitalization was 34 +/- 8.1 days. Treatment with PAIR and PAIR-PD took 1 and 3 days respectively. Complications of percutaneous methods were regarded as mild: hyperthermia (12), right-sided hydrothorax (6), subcapsular hematoma (1), urticaria (1). All the complications were treated conservatively and with US-assisted punctures. Long-term (from 6 months to 9 years) results of treatment were followed up in 26 patients: there were no recurrences. Suppuration of the residual cavity 6 months after the glue occlusion was eradicated by transcutaneous catheter drainage. One lethal outcome was not associated with complications of aspiration-catheter treatment. Both methods of transcutaneous treatment of hepatic hydatid cysts are effective and safe. A hypertonic solution of NaCl I produces a reliable antiscolecidal and sclerosis effect.

[Thermal trauma in a combination with respiratory tract burn]. / Termicheskaia travma v sochetanii s ozhogom dykhatel'nykh putei.

The detection rate of microbial contamination of burn wounds after flame burn was studied in dynamics to 3 weeks in 56 patients forming 2 similar groups: basic--with thermo-inhalation lesions (TIL) and control--without TIL. General area of skin burns in all the patients was less than 50% of the body surface, on average it was 40%, the area of deep burns was 23%. Cytological and bacteriologic methods were used for study microphlora in the sputum and in bronchoalveolar lavage. There were 186 cases of wound microbial contamination (gram-negative microorganisms--64.4% cases, gram-positive--34%, pathogenic fungi--1.6%). In 80% cases the microphlora of the wounds was polymicrobial, in 47% cases the microphlora were detected on the second week. Microphlora was detected 2 times more often in the basic group than in the control, corresponded to skin burn area; the greatest exceeding of critical level of wound contamination was revealed on the first 2 weeks. In TIL besides wound contamination, polymicrobial microphlora revealed in tracheobronchial tree, presented on the first 2 weeks with association of gram-positive and gram-negative microorganisms, combining on week 3 with fungal phlora. Early leukocytosis, not reducing during first 3 weeks of the study, due to intoxication by burn products, occurred in TIL. Early lymphopenia in TIL was the sign of resistant immunodeficiency.

[Clinico-laboratory effectiveness of modern ointments with a polyethylene glycol base in the treatment of purulent wounds]. / Kliniko-laboratornaia éffektivnost' sovremennykh mazei na poliétilenglikolevoi osnove pri lechenii gnoinykh ran.

The 25-year experience with the underbandage treatment of soft tissue purulent wounds of various location and genesis with modern ointments with polyethylene glycol as the basis was analyzed. Levocin, levomecole, dioxycole, 5-percent dioxydinic, 1-percent iodopyronic, 0.5-percent quinifuryl and furagel ointments proved to preserve their high activity against aerobic grampositive and gramnegative flora. 10-percent mafenide acetate ointment had a high selective effect on Pseudomonas aeruginosa. The new ointments nitacid and streptonitole containing nitazole and white streptocide were highly active against both aerobic and anaerobic infections. The use of the ointments with the polyethylene glycol as the basis made it possible to decrease 2 times the period of the patient hospitalization in surgical units and to shorten the terms of the systemic antibacterial therapy. The marked therapeutic effect of such ointments due to their high dehydrating capacity and broad antibacterial spectrum enabled to consider them as the drugs of choice in the local treatment of purulent wounds during the 1st phase of the wound process, trophic and decubic ulcers, infected burns, diabetic and atherosclerotic gangrene, furuncles, carbuncles, mastitis, etc. The ointments in the water soluble vehicle can be as well used with success for the prophylactic treatment of infected wounds after the suture. The multitarget effect of the ointments in the water soluble vehicle and their ability to prevent severe purulent complications permitted to consider them as the 1st order drugs in cases of emergency.

[The polychemotherapy and combined treatment of patients with stage-I and -II lymphogranulomatosis and unfavorable prognosis]. / Polikhimioterapiia i kombinirovannoe lechenie bol'nykh limfogranulematozom I--II stadii pri neblagopriiatnom prognoze.

As many as 104 patients with lymphogranulomatosis, clinical stages I-II, having an unfavourable prognosis were examined for the efficacy of polychemotherapy and combined treatment. Polychemotherapy was received by 47 patients, combined treatment (chemo- plus radiotherapy) by 57 patients. Complete remissions were attained significantly more often in patients given combined treatment (92%) as compared to patients on polychemotherapy alone (64%). After complete remissions relapses occurred in 60% of the patients on polychemotherapy and in 13% of the patients receiving combined treatment. The 7-year survival without any relapses was significantly higher (85%) in patients on combined therapy as compared to patients given polychemotherapy (36%). However, the 10-year survival appeared the same in both the groups (87 and 88%). Splenectomy affected neither the rate of relapses in both the groups nor the immediate treatment efficacy or survival.

[Cytotoxicity of effector cells of the peripheral blood, lymph nodes and spleen in Hodgkin's disease]. / Tsitotoksichnost' kletok-éffektorov perifericheskoi krovi, limfaticheskikh uzlov i selezenki pri bolezni Khodzhkina.

The mononuclear cells and T-lymphocytes of the blood, spleen and lymph nodes from 83 patients with Hodgkin's disease and 50 healthy donors were tested in assays for lectin-dependent (LD) and natural killer (NK) cytotoxic activity (CTA). On an average, peripheral blood T cell LD-CTA of patients did not differ from that of the donors. However, the CTA appeared to be dependent on the stage of the disease; in the IVth stage LD-CTA was decreased 2-fold. The LD-CTA was also dependent on the histological type of disease and the lowest level of LD-CTA (50% of the control level) was associated with the "lymphocyte depletion" type. The CTA of T-lymphocytes from the affected areas of the patients' spleen was more marked than that of the unaffected areas. Spleen cell CTA showed no other correlations. The CTA of lymphocytes from the affected lymph nodes was drastically lower than CTA of blood and spleen lymphocytes. The NK activity of the patients' blood and spleen lymphocytes was twice as less as the control level (healthy donors) and did not correlate with a stage and/or a histological type of the disease. It was assumed that in Hodgkin's disease the specific antitumor immunity remains mostly within normal and is decreased only in the last, terminal stage of the disease.

[The expression of antigens, detectable with ICO-series monoclonal antibodies, on the surfaces of cells forming granulocyte and macrophage colonies in semiliquid agar]. / Ekspressiia antigenov, vyiavliaemykh monoklonal'nymi antitelami serii IKO, na poverkhnosti kletok, obrazuiushchikh granulotsitarnye i makrofagal'nye kolonii v poluzhidkom agare.

The expression of antigens on granulocyte-macrophagal colony-forming cells of patients with nonhematological diseases was studied. Treatment of bone marrow cells with murine monoclonal antibodies ICO-1 and ICO-11 led to statistically significant inhibition of the number of growing colonies. Monoclonal antibodies ICO-02, ICO-10, ICO-GM-1 and ICO-G-2 had no such effect.

[Adhesion of the vitreous body to cured polymers]. / Issledovanie adgezii steklovidnogo tela na otverzhdennykh polimerakh.

The adhesive activity of several polymers as regard to vitreous body (VB) was examined by drying out the vitreous body on the surface of the materials under study. 3 types of polymers were compared with glass (silicon, polyurethan, polymethylmetacrilate). According to the reduction in adhesive activity as regard to VB the substances were ranged from silicon to glass. The authors believe that the mechanism of VB adhesion to polymers is linked to the absorption of gialuronic acid on their surface, which is part of VB. The above method is suggested for the selection of polymers used in the manufacturing of intraocular lenses.