Subject(s)
Glutathione S-Transferase pi/genetics , Glutathione Transferase/genetics , Hepatitis, Autoimmune/genetics , Polymorphism, Genetic/genetics , Adult , Antibodies, Antinuclear/blood , Autoantibodies/blood , Case-Control Studies , Chromosome Deletion , Cytoskeletal Proteins/immunology , Electrophoresis, Agar Gel , Female , Genotype , Humans , Male , Middle Aged , Muscle Proteins/immunology , Polymerase Chain ReactionABSTRACT
UNLABELLED: Autoimmune hepatitis (AIH) is a rare frequent, multiplex disorder with undefined etiology. Susceptibility to autoimmune hepatitis results from complex interactions between genetic and environmental factors. In this study, we investigated the involvement of C77G mutation in CD45 gene in patients with autoimmune hepatitis among Iranian population by genotyping this mutation in 70 patients and 140 healthy individuals. METHODS: : After amplifying exon 4 by Polymerase chain reaction, we genotyped this mutation with MspI restriction endonuclease among the studied population. RESULTS: : None of the cases with AIH was hetero or homozygote for C77G mutation. Controls had normal genotype except one of them who was heterozygote for C77G mutation. CONCLUSION: : Our results do not confirm the genetic link between C77G mutation and autoimmune hepatitis in Iranian population.
ABSTRACT
In this study, the frequencies of the common hemochromatosis gene mutations were assessed in 75 Iranian subjects with chronic hepatitis B infection. We found that the major C282Y mutation was significantly more frequent in subjects infected with hepatitis B virus (4%) than in 194 control subjects (0%, P=.02; Fisher's exact test).
