[The use of modified polystyrene plates in solid-phase immunoenzyme analysis for the diagnosis of infections]. / Ispol'zovanie modifitsirovannykh polistirolovykh planshetov v tverdofaznom immunofermentnom analize dlia diagnostiki nekotorykh infektsii.

The practical aspects of the theory of the adsorption of bioligands on the plastic surface have been considered and discussed. The possibility of standardizing polystyrene plates, as well as obtaining plates with the predetermined level of adsorption activity with respect to given antigens (antibodies), has been shown with the use of the patented technology for the modification of polystyrene plates. The data on the adsorption of antigens used as an experimental model (human and rabbit immunoglobulin fractions) are presented, and an increase in adsorption activity has been shown with the use of modified plates. For practical confirmation, some examples of using these plates in actual assay systems intended for the evaluation of HBsAg carriership and antibodies to HIV are given. A considerable increase (2- to 10-fold) of the signal from positive samples has been shown to occur, the signal from negative samples remaining practically unchanged.

[The use of the method of the complete advisability function for evaluating the quality and standardization of the plates in solid-phase EIA]. / Ispol'zovanie metoda polnoi funktsii zhelatel'nosti dlia otsenki kachestva i standartizatsii planshetov v tverdofaznon IFA.

An attempt to use the method of the complete advisability function for the evaluation of the quality of plates and their standardization in the enzyme-linked immunosorbent assay (ELISA) has been made. Correlations have been empirically established, thus making it possible to calculate the optimum proportions of different ELISA factors: the dose of the antigen (antibody) and the time of sorption, which ensures the best ELISA results with a given type of plates.

[Optimization of the parameters that permit improvement in plate sorption activity for solid-phase immunoenzyme analysis]. / Optimizatsiia nekotorykh parametrov, pozvoliaiushchikh uluchshit' sorbtsionnuiu aktivnost' planshet dlia tverdofaznogo immunofermentnogo analiza.

The possibility of increasing the sorption activity of polystyrene plates with an initially low capacity for sorption has been shown. The qualitative and quantitative parameters of the process of physical modification have been ascertained. The empirical formula for achieving the highest degree of the sorption activity of plates by their definite exposure to ultraviolet radiation has been obtained.