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2.
J Inherit Metab Dis ; 32(3): 424-40, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19387866

ABSTRACT

Fabry disease is an X-linked lysosomal storage disorder caused by mutations in the gene encoding alpha-galactosidase A (alpha-Gal A), with consequent accumulation of its major glycosphingolipid substrate, globotriaosylceramide (GL-3). Over 500 Fabry mutations have been reported; approximately 60% are missense. The iminosugar 1-deoxygalactonojirimycin (DGJ, migalastat hydrochloride, AT1001) is a pharmacological chaperone that selectively binds alpha-Gal A, increasing physical stability, lysosomal trafficking, and cellular activity. To identify DGJ-responsive mutant forms of alpha-Gal A, the effect of DGJ incubation on alpha-Gal A levels was assessed in cultured lymphoblasts from males with Fabry disease representing 75 different missense mutations, one insertion, and one splice-site mutation. Baseline alpha-Gal A levels ranged from 0 to 52% of normal. Increases in alpha-Gal A levels (1.5- to 28-fold) after continuous DGJ incubation for 5 days were seen for 49 different missense mutant forms with varying EC(50) values (820 nmol/L to >1 mmol/L). Amino acid substitutions in responsive forms were located throughout both structural domains of the enzyme. Half of the missense mutant forms associated with classic (early-onset) Fabry disease and a majority (90%) associated with later-onset Fabry disease were responsive. In cultured fibroblasts from males with Fabry disease, the responses to DGJ were comparable to those of lymphoblasts with the same mutation. Importantly, elevated GL-3 levels in responsive Fabry fibroblasts were reduced after DGJ incubation, indicating that increased mutant alpha-Gal A levels can reduce accumulated substrate. These data indicate that DGJ merits further evaluation as a treatment for patients with Fabry disease with various missense mutations.


Subject(s)
1-Deoxynojirimycin/analogs & derivatives , Fabry Disease/pathology , alpha-Galactosidase/metabolism , 1-Deoxynojirimycin/pharmacology , Cell Line , Drug Evaluation, Preclinical , Enzyme Activation/drug effects , Fabry Disease/enzymology , Fabry Disease/metabolism , Fibroblasts/drug effects , Fibroblasts/metabolism , Fibroblasts/pathology , Half-Life , Humans , Lymphocytes/drug effects , Lymphocytes/metabolism , Lymphocytes/pathology , Male , Models, Molecular , Molecular Chaperones/pharmacology , Mutation, Missense/physiology , Up-Regulation/drug effects , alpha-Galactosidase/chemistry , alpha-Galactosidase/genetics
3.
Transplant Proc ; 40(7): 2262-7, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18790208

ABSTRACT

OBJECTIVE: The objective of this prospective study was to evaluate the effect of conversion from mycophenolate mofetil (MMF) to enteric-coated mycophenolate sodium (EC-MPS) in renal transplant recipients with gastrointestinal tract (GI) reverse effects using patient-reported outcomes instrument. METHODS: A multicenter, open-label, prospective study was undertaken in renal transplant recipients treated with MMF. In patients experiencing GI tract symptoms, treatment was changed to equimolar EC-MPS (myfortic). At baseline and visit 2 (4-6 weeks after baseline), patients completed the Gastrointestinal Symptom Rating Scale (GSRS) and Gastrointestinal Quality of Life Index (GIQLI), and physicians completed the Overall Treatment Effect (OTE) scale at visit 2. A difference of 0.5 or greater in the OTE score is indicative of clinical relevance. RESULTS: Of 154 patients screened, 118 fulfilled the inclusion or exclusion criteria. Eighty-five men and 33 women with a mean age of 41.6 years participated in this study. Median time since transplantation was 12 months. Mean (SD) dose of MMF reported at baseline was 1209.4 (422.89) mg/d. More than 50% of patients reported MMF-associated nausea, dyspepsia, and abdominal pain. After conversion to an equimolar dose of EC-MPS, patients showed improvement in GI symptoms. This benefit was predominantly observed in patients with moderate to severe symptoms at baseline. On the GSRS, patients reported a significant (P < .05) reduction in symptom burden across all parameters (reflux, 36%; diarrhea, 38%; indigestion, 36%; constipation, 28%; and abdominal pain, 40%). On the GIQLI also, significant (P < .05) improvement was reported (symptoms, 18%; emotional status, 22%; physical functioning, 21%, and use of medical treatment, 18%). On the OTE scale, 84.7% of patients reported improvement in GI symptoms. CONCLUSION: In patients with moderate to severe GI symptoms, changing treatment from MMF to EC-MPS significantly reduces GI-related symptom burden and improves GI-specific quality of life.


Subject(s)
Gastrointestinal Diseases/chemically induced , Kidney Transplantation/immunology , Mycophenolic Acid/analogs & derivatives , Mycophenolic Acid/therapeutic use , Adult , Female , Gastrointestinal Diseases/classification , Gastrointestinal Diseases/drug therapy , Humans , Immunosuppressive Agents/adverse effects , Immunosuppressive Agents/therapeutic use , Longitudinal Studies , Male , Middle Aged , Mycophenolic Acid/administration & dosage , Mycophenolic Acid/adverse effects , Prospective Studies , Surveys and Questionnaires , Tablets, Enteric-Coated , Tissue Donors/statistics & numerical data , Treatment Outcome
4.
Indian J Microbiol ; 47(2): 132-8, 2007 Jun.
Article in English | MEDLINE | ID: mdl-23100655

ABSTRACT

Statistically based experimental design was employed for the optimization of fermentation conditions for maximum production of enzyme tannase from Aspergillus niger. Central composite rotatable design (CCRD) falling under response surface methodology (RSM) was used. Based on the results of 'one-at-a-time' approach in submerged fermentation, the most influencing factors for tannase production from A. niger were concentrations of tannic acid and sodium nitrate, agitation rate and incubation period. Hence, to achieve the maximum yield of tannase, interaction of these factors was studied at optimum production pH of 5.0 by RSM. The optimum values of parameters obtained through RSM were 5% tannic acid, 0.8% sodium nitrate, 5.0 pH, 5 × 10(7) spores/50mL inoculum density, 150 rpm agitation and incubation period of 48 h which resulted in production of 19.7 UmL(-1) of the enzyme. This activity was almost double as compared to the amount obtained by 'one-at-a-time' approach (9.8 UmL(-1)).

5.
J Appl Microbiol ; 100(6): 1348-54, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16696683

ABSTRACT

AIM: Development and optimization of an efficient and inexpensive medium for succinic acid production by Escherichia coli under anaerobic conditions. METHODS AND RESULTS: Initially, 0.8 gl(-1) of succinic acid was produced in 60 h in 300-ml medium. On optimization, glucose and peptone were replaced by cane molasses and corn steep liquor. Three hundred ml of this medium was inoculated with 4% (v/v) of seed inoculum, incubated at 39 degrees C for 72 h, resulted in 7.1 gl(-1) of succinic acid in 36 h. Scale up in a 10-l fermentor under conditions of controlled pH and continuous CO2 supply in this medium resulted in 17 gl(-1) of succinic acid in 30 h. CONCLUSIONS: A ninefold increase in succinic acid production was obtained in 500-ml anaerobic bottles with optimized medium having cane molasses and corn steep liquor as against initial medium containing glucose and peptone. However, a subsequent scale up in a 10-l fermentor resulted in a 2.5-fold increase in succinic acid production as against optimized medium used in 500-ml anaerobic bottles. SIGNIFICANCE AND IMPACT OF THE STUDY: Succinic acid production was enhanced in medium consisting of inexpensive carbon and nitrogen sources in a shorter span of time.


Subject(s)
Bioreactors/economics , Escherichia coli/metabolism , Industrial Microbiology/methods , Saccharum , Succinic Acid/metabolism , Zea mays , Animals , Buffaloes , Cost-Benefit Analysis , Culture Media , Fermentation , Rumen/microbiology , Succinic Acid/analysis
6.
Pediatr Surg Int ; 17(5-6): 452-4, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11527187

ABSTRACT

Agenesis of the cranial bones, a rare condition usually incompatible with life, was seen in a neonate. Embryogenesis and management of the condition are discussed. The defect heals with conservative treatment.


Subject(s)
Skull/abnormalities , Anti-Bacterial Agents/therapeutic use , Bandages , Congenital Abnormalities/diagnostic imaging , Congenital Abnormalities/therapy , Framycetin/therapeutic use , Humans , Infant, Newborn , Male , Radiography
8.
J Med Chem ; 42(12): 2180-90, 1999 Jun 17.
Article in English | MEDLINE | ID: mdl-10377223

ABSTRACT

Novel potent and selective diarylimidazole inhibitors of p38 MAP (mitogen-activated protein) kinase are described which have activity in both cell-based assays of tumor necrosis factor-alpha (TNF-alpha) release and an animal model of rheumatoid arthritis. The SAR leading to the development of selectivity against c-Raf and JNK2alpha1 kinases is presented, with key features being substitution of the 4-aryl ring with m-trifluoromethyl and substitution of the 5-heteroaryl ring with a 2-amino substituent. Cell-based activity was significantly enhanced by incorporation of a 4-piperidinyl moiety at the 2-position of the imidazole which also enhanced aqueous solubility. In general, oral bioavailability of this class of compounds was found to be poor unless the imidazole was methylated on nitrogen. This work led to identification of 48, a potent (p38 MAP kinase inhibition IC50 0.24 nM) and selective p38 MAP kinase inhibitor which inhibits lipopolysaccharide-stimulated release of TNF-alpha from human blood with an IC50 2.2 nM, shows good oral bioavailability in rat and rhesus monkey, and demonstrates significant improvement in measures of disease progression in a rat adjuvant-induced arthritis model.


Subject(s)
Aminopyridines/chemical synthesis , Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors , Enzyme Inhibitors/chemical synthesis , Imidazoles/chemical synthesis , Mitogen-Activated Protein Kinases , Administration, Oral , Aminopyridines/chemistry , Aminopyridines/pharmacokinetics , Aminopyridines/pharmacology , Animals , Arthritis, Experimental/drug therapy , Biological Availability , Drug Design , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacokinetics , Enzyme Inhibitors/pharmacology , Female , Humans , Imidazoles/chemistry , Imidazoles/pharmacokinetics , Imidazoles/pharmacology , Macaca mulatta , Mice , Rats , Stimulation, Chemical , Structure-Activity Relationship , Tumor Necrosis Factor-alpha/biosynthesis , p38 Mitogen-Activated Protein Kinases
9.
Pediatr Surg Int ; 15(8): 591-2, 1999.
Article in English | MEDLINE | ID: mdl-10631745

ABSTRACT

A case of scaphoid megalourethra associated with the posterior urethral valves was seen in a 4-year-old male. The embryology of the anomaly and its management are discussed with a review of the literature.


Subject(s)
Urethra/abnormalities , Urethra/pathology , Child, Preschool , Dilatation, Pathologic , Humans , Male
11.
Indian J Pediatr ; 64(6): 896-8, 1997.
Article in English | MEDLINE | ID: mdl-10771937

ABSTRACT

Gastric teratoma, a very rare tumor of infancy is essentially benign. Immature (embryonic) elements have been described in only three of the sixty-six cases described previously. This report concerns with one "immature" gastric teratoma seen in infancy.


Subject(s)
Neoplasm Recurrence, Local/pathology , Stomach Neoplasms/pathology , Teratoma/pathology , Biopsy, Needle , Fatal Outcome , Humans , Infant, Newborn , Laparotomy , Male , Neoplasm Recurrence, Local/diagnosis , Neoplasm Recurrence, Local/surgery , Reoperation , Stomach Neoplasms/diagnosis , Stomach Neoplasms/surgery , Teratoma/diagnosis , Teratoma/surgery
12.
Ren Fail ; 19(6): 763-70, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9415933

ABSTRACT

A prospective study of all new cases of chronic renal failure (CRF) including inservice referrals was done at our hospital over a period of 1 year from May 1994 to April 1995. The diagnosis of CRF was based on clinical, laboratory, and radiological features. Kidney biopsies were done when indicated. The patients were subdivided into various etiologic groups of primary renal disease according to standard criteria. There were a total of 835 cases of CRF with a median age of 43 years (range 10 days to 90 years); 67.8% of them were men. Glomerulonephritis (28.6%), diabetic nephropathy (23.2%), and interstitial nephritis (16.5%) were the most common causes of CRF, followed by obstructive nephropathy (6.4%), benign nephrosclerosis (4.1%), and polycystic kidney disease (2%). However, in patients more than 40 years of age, diabetic nephropathy was the most common cause (36.8%). The cause of CRF was unknown in 16.2% of the cases. One hundred twenty-one patients (14.5%) had an acute deterioration of their underlying renal dysfunction at presentation. This was most commonly due to accelerated hypertension (26.1%), infection (22.4%), volume depletion (20.1%), and drugs (14.9%). Anti-inflammatory drugs were the most common drugs responsible for the acute decline in renal function. One year after their initial presentation, of the 512 patients (61.3%) with end stage renal disease, 12.5% had died, 17% had received a kidney allograft, 12.7% were on some form of maintenance dialysis, and 295 patients were lost to follow-up. Of the 323 patients with less severe illness, 7 died, 209 were on outpatient treatment, and 107 patients were lost to follow-up. We conclude that the pattern of CRF in India does not differ greatly from that in the developed countries. However, it carries a poorer prognosis due to late referral and limited availability and affordability of renal replacement therapy in India.


Subject(s)
Kidney Failure, Chronic/epidemiology , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Diabetic Nephropathies/complications , Female , Glomerulonephritis/complications , Humans , India/epidemiology , Infant , Infant, Newborn , Kidney Failure, Chronic/etiology , Kidney Failure, Chronic/mortality , Male , Middle Aged , Nephritis, Interstitial/complications , Nephrosclerosis/complications , Polycystic Kidney Diseases/complications , Prognosis , Prospective Studies
13.
Talanta ; 43(7): 1069-73, 1996 Jul.
Article in English | MEDLINE | ID: mdl-18966582

ABSTRACT

A simple coprecipitation technique for the quantitative separation of the antimony present in impure zinc sulphate electrolyte followed by its voltammetric determination is described. Antimony in microgram levels is separated from the matrix zinc sulphate solution, which contains higher levels of copper, lead and cadmium, and is subsequently determined by differential pulse anodic stripping voltammetry (DPASV) in 3 M hydrochloric acid. Hydrous manganese dioxide is employed as the collector. This procedure, which effects considerable saving in time, is of comparable accuracy to the conventional spectrophotometric method using the antimony-rhodamine B complex. A series of synthetic zinc sulphate solutions spiked with known amounts of antimony as well as plant solutions gave near theoretical values.

14.
J Interferon Cytokine Res ; 15(3): 243-8, 1995 Mar.
Article in English | MEDLINE | ID: mdl-7584670

ABSTRACT

A potent, reversible, tetrapeptide inhibitor of interleukin-1 beta converting enzyme (ICE), L-709,049, has been shown to suppress the in vitro production of mature IL-1 beta. We now report that this inhibitor also effectively suppresses the production of mature IL-1 beta in a murine model of endotoxic shock. Intraperitoneal administration of L-709,049 reduced the elevations of IL-1 beta in the plasma and peritoneal fluid of mice treated with LPS in a dose-related manner (ED50 = 2 +/- 0.9 mg/kg). LPS-induced elevations in IL-1 alpha and IL-6 in these mice were unaffected, indicating that the inhibitor specifically affected IL-1 beta production. Immunoblot analysis of plasma and peritoneal fluid indicated that L-709,049 suppressed the formation of mature IL-1 beta production in vivo. When mouse blood was incubated in vitro with LPS, IL-1 beta was released into the plasma. This assay was used to determine ex vivo the activity of an ICE inhibitor in the blood following its administration to mice. Blood obtained 15 minutes after ip administration of 10 mg/kg of L-709,049 to mice produced 80% less IL-1 beta than control blood, and IL-1 beta production returned to control levels in blood obtained 30 minutes after injection of this inhibitor. In addition, the capacity of the blood plasma obtained from these animals to prevent the cleavage of a synthetic substrate by ICE disappeared within 1 h of ip administration of 50 mg/kg of inhibitor.


Subject(s)
Cysteine Endopeptidases/metabolism , Cysteine Proteinase Inhibitors/pharmacology , Endotoxins/pharmacology , Interleukin-1/biosynthesis , Oligopeptides/pharmacology , Shock, Septic/immunology , Animals , Ascitic Fluid/immunology , Caspase 1 , Dose-Response Relationship, Drug , Humans , In Vitro Techniques , Interleukin-1/blood , Kinetics , Lipopolysaccharides/pharmacology , Mice , Oligopeptides/pharmacokinetics , Propionibacterium acnes/immunology
17.
Indian Pediatr ; 30(11): 1363-6, 1993 Nov.
Article in English | MEDLINE | ID: mdl-8039867

ABSTRACT

We reviewed our experience with 1369 inguinal herniotomies in 1340 children performed over the last one decade. Different grades of surgeons were assigned work according to the complexity of cases. Except for the minor scrotal hematoma, other complications were hardly seen. Recurrences were seen in only 2 cases. Careful training and supervision of junior staff in the technique of inguinal herniotomy has led to results that compare favorably to those of specialized units in developed countries.


Subject(s)
Hernia, Inguinal/surgery , Child , Child, Preschool , Clinical Competence , Female , Humans , India , Infant , Infant, Newborn , Male
18.
Indian Pediatr ; 30(9): 1149-53, 1993 Sep.
Article in English | MEDLINE | ID: mdl-8125610

ABSTRACT

This presentation deals with 110 surgically proven cases of abdominal tuberculosis in the pediatric age group. The protean clinical profiles and complications of the disease entity made the clinical diagnosis difficult; the investigations were also found non-pathognomonic. The most common type of pathology seen was adhesive variety followed by nodal type. Strictures of the small bowel were uncommon and hyperplastic variety was rarely seen in the present series. The pathogenesis relating to various varieties has been suggested based on the vast experience from a single institution.


Subject(s)
Abdomen , Tuberculosis, Gastrointestinal/diagnosis , Abdomen/pathology , Adolescent , Antitubercular Agents/therapeutic use , Child , Child, Preschool , Combined Modality Therapy , Drug Therapy, Combination , Female , Humans , Hyperplasia/diagnosis , Hyperplasia/etiology , Hyperplasia/therapy , Infant , Laparotomy , Male , Tissue Adhesions/diagnosis , Tissue Adhesions/etiology , Tissue Adhesions/therapy , Tuberculosis, Gastrointestinal/complications , Tuberculosis, Gastrointestinal/therapy
20.
Indian Pediatr ; 30(5): 689-94, 1993 May.
Article in English | MEDLINE | ID: mdl-8282403

ABSTRACT

The clinicopathological features of 75 children under the age of 12 years with teratomas are reviewed. Tumors arose in the following anatomic sites: sacrococcygeum (n = 49), ovary (n = 10), Testis (n = 5), oral cavity (n = 3), retroperitoneum (n = 2) and others (n = 6). Fifty five (74%) presented within the first year of life. Excluding the gonadal tumors, male-female ratio was 2:5. Majority of the tumors had only mature tissues. Such patients and those 9 patients in whom the histology was not specified, underwent excision alone and had 95% early survival rates. Five patients had admixture of mature and immature tumors. Nine patients had malignant tissues. Germ cell tumors containing only malignant component, but no mature or immature teratomatous tissues were excluded from the series. The patients with immature and malignant tissues underwent multimodal therapy including surgical excision, multiagent chemotherapy (VAC regimen) and at times radiotherapy. Mortality in patients with immature and malignant teratomas was 20 and 66.7%, respectively. Besides histology, the only factor which affected prognosis, especially in case of sacrococcygeal teratomas was the age at the time of presentation. Our experience highlights the importance of early recognition and complete surgical excision of teratomas in the pediatric age group.


Subject(s)
Teratoma/pathology , Child , Child, Preschool , Coccyx , Female , Follow-Up Studies , Head and Neck Neoplasms/pathology , Humans , Infant , Male , Ovarian Neoplasms/pathology , Sacrum , Sex Factors , Spinal Neoplasms/pathology , Testicular Neoplasms/pathology
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