Caffeine Consumption in Polish Adults: Development and Validation of a Polish Questionnaire for Assessing Caffeine Intake.

Objective: Caffeine is one of the oldest natural substances consumed by people. Its consumption in Poland has not been well described. The aim of this study was to design and validate an online food frequency questionnaire (FFQ) on caffeine intake and to use it to estimate caffeine consumption in Polish adults.Method: The FFQ was prepared and validated in a pilot study. The intake assessment was conducted in 2019-2020 on 372 respondents, aged 18 to 60 years. The FFQ included products such as coffee, tea, energy drinks, and carbonated drinks, as well as supplements and chocolate.Results: We showed good repeatability of the FFQ and it was considered a valid tool. The mean total caffeine intake among all participants was 426.7 mg ± 283.4 mg/day of all sources; in women, it was 446.4 mg ± 306.2 mg/day, while in men, it was 394.1 ± 236.4 mg/day. Forty-three percent of the respondents consumed more than 400 mg of caffeine/day. Coffee was the main source of caffeine and contributed to 65% of total caffeine consumption. Women consumed 90% more green tea than men (p < 0.01). Overweight and obese people have 20% greater total caffeine intake (p = 0.01) and consumed 20% more coffee (p = 0.02) and 30% more black tea (p = 0.01) than people of normal weight.Conclusions: Average caffeine consumption among Polish adults slightly exceeds the safe consumption dose established by the European Food Safety Authority. Body weight status can differentiate caffeine intake.

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview.

Ensiling is one of the best known method to preserve fodder. The forage before ensiling intended for silages usually contains a low number of lactic acid bacteria (LAB), so it is necessary to apply starter cultures of selected strains. Traditionally, LAB starter cultures were applied to lower the pH by producing lactic acid and to inhibit the growth of undesirable epiphytic microorganisms by competing for nutrients. Nowadays, LAB inoculants have become an effective tool for creating microbial quality of silages by selecting species with extraordinary features. Epiphytic microflora characteristic of plant material used for the production of silages and the sources of undesirable microflora in the ensiling process are discussed. This review focuses on the most frequently studied issues related to the microbial silage quality and the recent trends in increasing the quality by LAB inoculants, with respect to recent directions for selecting types of modern LAB for inoculation. Among them, the main trends described were prevention of the growth of filamentous fungi and detoxification of mycotoxins by LAB inoculants, inhibition of yeast growth by LAB present in preparations and limiting the development of pathogenic bacterial microflora through controlled fermentation with the participation of LAB and the presence of their metabolites.

Dehydroepiandrosterone administration increased trabecular mass and dihydrotestosterone levels in the cancellous region of the tibia in young female rats.

The aim of the present study was to determine whether dehydroepiandrosterone (DHEA) administration affects bone mass and local sex hormone levels in the cancellous region of young female rats. Eleven female rats (6 weeks old) were randomly divided into 2 groups: control rats (CON, n=5) and rats treated with DHEA (DHEA, n=6). DHEA dissolved in sesame oil was administered to the DHEA group intraperitoneally at 20 mg DHEA/kg body weight, and the CON group was treated with vehicle only (sesame oil, 0.5 ml). The rats were treated with DHEA or vehicle for 3 consecutive days, followed by 1 day of no treatment. The experimental period was 8 weeks. According to dual-energy X-ray absorptiometry and high-resolution microcomputed tomography data, the DHEA group exhibited increased trabecular bone mineral density (BMD), bone volume, and tibial thickness compared to the findings in the CON group, whereas no effect was observed on cortical BMD or morphometry. The concentrations of free testosterone and estradiol in the cancellous region of the tibia did not differ between the 2 groups, but the DHT concentration was significantly higher in the DHEA group than in the CON group. These findings suggest that an increase in local DHT levels may stimulate an increase in trabecular bone mass during growth phases in female rats.