ABSTRACT
INTRODUCTION: The therapeutic method for many malignant and non-malignant diseases is hematopoietic stem cell transplantation (HSCT), but it is not always fully successful in all patients. Indeed, HSCT can be influenced by a variety of factors. Here we reviewed the effect of microRNAs (miRs) on HSCT-related outcomes, like survival, infections, relapse, engraftment, and so on, systematically. METHOD: WOS, Scopus, PubMed, Google Scholar, and ProQuest databases were searched. The PRISMA guideline was performed, and 24 studies were included through quality assessment. Classified data extraction was done based on the type of disease. RESULTS: The systematic review identified 47 miRs effective on HSCT. The role of miRs as tumor suppressors or oncogenes is reported in acute myeloblastic and lymphoblastic leukemia patients undergoing HSCT due to their effects on overall or event-free survival. Additionally, relapse after HSCT in multiple myeloma is correlated with miRs expression. Also, recovery from post-autologous HSCT cytopenia or platelet and neutrophil engraftment can be influenced by miRs. We highlighted here reports on specific miRs. CONCLUSION: We reported prognostic miRs for in-depth clinical management of the HSCT process and its outcomes. Also, miRs are introduced for the prevention of HSCT-related complications, and future studies are suggested to evaluate personalized medicine's utilization of miRs in therapeutic methods like HSCT in neoplasia.
Subject(s)
Hematopoietic Stem Cell Transplantation , MicroRNAs , Humans , Hematopoietic Stem Cell Transplantation/methods , MicroRNAs/genetics , Prognosis , Treatment Outcome , Multiple Myeloma/genetics , Multiple Myeloma/therapyABSTRACT
BACKGROUND: Sepsis is a life-threatening disorder with no definitive cure. Preclinical studies suggest that extracellular vesicles derived from mesenchymal stromal cells (EV-MSCs) can mitigate inflammatory conditions, potentially leading to increased survival and reduced organ dysfunction during sepsis. Our aim to conduct this systematic review and meta-analysis is assessing the EV-MSCs therapeutic efficacy in sepsis. METHODS: PubMed, Embase, Scopus, WOS and ProQuest databases and also Google Scholar search engine were searched for published articles. We used hazard ratio (HR) and standardized mean difference (SMD) as effect sizes to evaluate the therapeutic effect of EV-MSCs on survival rate and determine their effect on reducing organ dysfunction, respectively. Finally, we employed GRADE tool for preclinical animal studies to evaluate certainty of the evidence. RESULTS: 30 studies met the inclusion criteria for our article. Our meta-analysis results demonstrate that animals treated with MSC-EVs have better survival rate than untreated animals (HR = 0.33; 95% CI: 0.27-0.41). Our meta-analysis suggests that EV-MSCs can reduce organ dysfunctions in sepsis, such as the lung, kidney, and liver. Additionally, EV-MSCs decrease pro-inflammatory mediators like TNF-α, IL-1ß, and IL-6. CONCLUSION: Our results indicate that EV-MSCs can be as promising therapy for sepsis management in animal models and leading to increased survival rate and reduced organ dysfunction. Furthermore, our study introduces a novel tool for risk of bias assessment and provides recommendations based on various analysis. Future studies with aiming to guide clinical translation can utilize the results of this article to establish stronger evidence for EV-MSC effectiveness.
Subject(s)
Extracellular Vesicles , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells , Sepsis , Sepsis/therapy , Extracellular Vesicles/transplantation , Extracellular Vesicles/metabolism , Animals , Mesenchymal Stem Cells/metabolism , Mesenchymal Stem Cells/cytology , Humans , Disease Models, AnimalABSTRACT
Unlike improved treatment response in multiple myeloma (MM), the mortality rate in MM is still high. The study's aim is to investigate the potential role of circRNAs as a new biomarker for diagnosis, prognosis, and clinicopathological features of MM. We identified studies through Web of Science, Scopus, PubMed and ProQuest databases, and Google Scholar to August 2022. The SEN, SPE, PLR, NLR, DOR, and AUC were combined to investigate the diagnostic performance of circRNAs in MM. Also, HR and RR were used for prognostic and clinicopathological indicators, respectively. 12 studies for prognosis, 9 studies about diagnosis, and 13 studies regarding clinicopathological features. The pooled SEN, SPE, DOR, and AUC were 0.82, 0.76, 14.70, and 0.86, respectively for the diagnostic performance of circRNAs. For the prognostic performance, oncogene circRNAs showed a poor prognosis for the patients (HR = 3.71) and tumor suppressor circRNAs indicated a good prognosis (HR = 0.31). Finally, we discovered that dysregulation of circRNAs is associated with poor clinical outcomes in beta-2-microglobulin (RR = 1.56), Durie-Salmon stage (RR = 1.36), and ISS stage (RR = 1.79). Furthermore, the presence of del(17p) and t(4;14) is associated with circRNA dysregulation (RR = 1.44 and 1.44, respectively). Our meta-analysis demonstrates that the expression analysis of circRNAs is valuable for MM's diagnosis and prognosis determination. Also, dysregulation of circRNAs is associated with poor clinicopathological features and can be used as the applicable biomarkers for evaluating treatment effectiveness.