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Vopr Virusol ; 42(1): 44-7, 1997.
Article in Russian | MEDLINE | ID: mdl-9103046

ABSTRACT

The authors have developed enzyme immunoassay (EIA) for detecting antimeasles antibodies in the serum and compared it to routine methods. Preparation and purification of measles virus antigen used in EIA and optimal conditions of the reaction are described. Results of the routine methods and those of EIA correlated. EIA was used to determine titers of antimeasles antibodies in the sera of vaccinated children. 3-4 years after the vaccination the antibodies were undetectable in the sera of 23% of children.


Subject(s)
Antibodies, Viral/analysis , Measles virus/immunology , Animals , Child, Preschool , Chlorocebus aethiops , Guinea Pigs , Humans , Immunoenzyme Techniques , Measles virus/ultrastructure , Methods , Microscopy, Electron , Rabbits , Vero Cells
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