Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 9 de 9
Filter
Add more filters











Database
Language
Publication year range
1.
World J Microbiol Biotechnol ; 38(6): 97, 2022 Apr 28.
Article in English | MEDLINE | ID: mdl-35478267

ABSTRACT

In the recent past, the mass production of arbuscular mycorrhizal (AM) fungi has bloomed into a large biofertilizer industry. Due to their obligate symbiotic nature, these fungi are propagated on living roots in substrate-based pot cultures and RiTDNA in in vitro or root organ culture systems. The quality assessment of AM inocula remains critical for the production and efficacy evaluation of AM fungi. The vigour of AM inocula are assessed through microscopic methods such as inoculum potential, infectivity potential/infection units, most probable number (MPN) and spore density. These methods marginally depend on the researcher's skill. The signature lipids specific to AM fungi, e.g. 16:1ω5cis ester-linked, phospholipid, and neutral lipid fatty acids provide more robustness and reproducibility. The quantitative real-time PCR of AM fungal taxa specific primers and probes analyzing gene copy number is also increasingly used. This article intends to sensitize AM fungal researchers and inoculum manufacturers to various methods of assessing the quality of AM inocula addressing their merits and demerits. This will help AM producers to fulfil the regulatory requirements ensuring the supply of high-quality AM inocula to end-users, and tap a new dimension of AM research in the commercial production of AM fungi and its application in sustainable plant production systems.


Subject(s)
Mycorrhizae , Fertilizers , Mycorrhizae/genetics , Plants , Reproducibility of Results , Symbiosis
2.
J Helminthol ; 94: e27, 2019 Jan 31.
Article in English | MEDLINE | ID: mdl-30702050

ABSTRACT

The present study determined the prevalence of hydatid cysts in different organs of slaughtered hilly 'Gaddi' breed small ruminants-sheep (n = 230) and goats (n = 197)-in Kangra Valley of the north-western Himalayas, India. Hydatid cysts were found in 12.2% (n = 28) of sheep and 10.7% (n = 21) of goats. Pulmonary echinococcosis was more prevalent in slaughtered sheep and goats (sheep 56.36%; goats 62.90%) than hepatic echinococcosis (sheep 43.64%; goats 37.10%). Fertility rates were higher in hepatic (81.25%) and pulmonary cysts of sheep (83.87%) compared to goats. Molecular identification and genotypic characterization of Echinococcus granulosus isolates were based on mitochondrial cytochrome oxidase 1 gene (mtCO1). The genotypic characterization identified the isolated strain to be closely related to the G7 genotype. Histopathological examination revealed a thick coat of granulation tissue, causing fibrosis and inflammatory reaction composed of fibroblasts and mononuclear cells around the cysts. In the liver, hepato-cellular degeneration was prominent at the periphery of the cysts. The present study highlights the molecular confirmation and phylogenetic analysis of E. granulosus isolates with the prevalence of hydatidosis in a naïve host species and in an unexplored region. The findings are of significant medical and veterinary importance regarding development of control measures to check dissemination of hydatidosis.


Subject(s)
Echinococcosis/veterinary , Echinococcus granulosus/isolation & purification , Goat Diseases/parasitology , Sheep Diseases/parasitology , Abattoirs , Animals , Echinococcosis/epidemiology , Echinococcosis/parasitology , Echinococcosis/pathology , Echinococcus granulosus/classification , Echinococcus granulosus/genetics , Genotype , Goat Diseases/epidemiology , Goat Diseases/pathology , Goats , India/epidemiology , Liver/parasitology , Liver/pathology , Phylogeny , Sheep , Sheep Diseases/epidemiology , Sheep Diseases/pathology
3.
J Parasit Dis ; 40(2): 227-9, 2016 Jun.
Article in English | MEDLINE | ID: mdl-27413283

ABSTRACT

A total of 246 faecal/scat samples of the dogs were screened by direct and floatation concentration technique to study the gastrointestinal (GI) tract parasitism in dogs of Palampur, Himachal Pradesh, India. Detailed coprological examination targeting different seasons, age groups and living styles of the dogs revealed an overall 28.04 % of GI parasitism with highest prevalence in summer season (37.87 %). Stray dogs harbored 47.29 % GI parasites in comparison to 19.19 % of pet dogs. Highest prevalence of GI parasitism was observed in the pups, below 3 months of age (39.13 %), followed by the dogs with the age ranging from 3 months to 1 year (26.38 %) and lowest in dogs of the age ranging from 1 to 3 years (6.77 %). Amongst all the parasites, Toxocara canis (44.93 %) infection was highest, followed by Dipylidium caninum (17.39 %) and hookworms (15.94 %).

4.
Cell Mol Biol (Noisy-le-grand) ; 61(3): 57-62, 2015 Jun 24.
Article in English | MEDLINE | ID: mdl-26107501

ABSTRACT

Lead (Pb) is a highly toxic heavy metal for both plants and animals; the environment is increasingly polluted with heavy metals and reduces crop productivity. Plants possess homeostatic mechanisms that allow them to keep correct concentrations of essential metal ions in cellular compartments and to minimize the damaging effects of an excess of nonessential ones. One of their adverse effects on plants are the generation of harmful active oxygen species, leading to oxidative stress and the antioxidative activity seems to be of fundamental importance for adaptive response of plant against environmental stress. The present study explores the effects of lead (soil treated twice/ week) with (10, 30 and 60 mM) on the specific activities of phosphatases which might lead to reducing power assay in (Triticum aestivum PBW344) seedling. A significant decrease in the redox potential of shoot compared to root was observed at the similar concentration of lead. A similar trend on leaves was also noted. Acid and alkaline phosphatase activities were significantly higher in roots than in shoot at all the three concentration of lead i.e. 10, 30 and 60 mM, compared to controls. The above mentioned changes were more pronounced at 60 mM concentration of lead than two other concentrations. These results lead us to suggest that increased lead concentration in soil might lead to adverse effects on plant growth and phosphatase activities.


Subject(s)
Alkaline Phosphatase/metabolism , Lead/metabolism , Triticum/enzymology , Acid Phosphatase/metabolism , Germination/drug effects , Lead/chemistry , Lead/toxicity , Oxidation-Reduction , Plant Leaves/enzymology , Plant Leaves/metabolism , Plant Roots/enzymology , Plant Roots/metabolism , Plant Shoots/enzymology , Plant Shoots/metabolism , Seedlings/drug effects , Seedlings/growth & development , Soil/chemistry , Triticum/growth & development
5.
Pak J Biol Sci ; 17(4): 594-6, 2014 Apr.
Article in English | MEDLINE | ID: mdl-25911855

ABSTRACT

Coriander commonly known as Dhania or Chinese parsley is generally grown for its use in soups, salads, dressing vegetables, seasoning and chutney. Effect of two phytohormones viz. GA3 and 2,4-D on seed germination, seedling growth and various physiological and biochemical parameters were studied. The hormones were applied individually in different concentrations (10, 50 and 100 µM concentrations). Both the hormones enhanced the germination percentage, seedling growth (root and shoot length), leaf area, chlorophyll and carotenoid content. The application of these hormones also decreased the germination time. Maximum germination, shoot length, leaf area and carotenoid content was observed in 100 µm concentration of GA3. Root length, chl. a and chl. b was maximum in 50 µM of 2,4-D and 100 µm GA3, respectively. The application of two hormones exhibited a marked increase on all the parameters studied as compared to the control.


Subject(s)
Coriandrum/drug effects , Germination/drug effects , Indoleacetic Acids/pharmacology , Plant Growth Regulators/pharmacology , Seedlings/drug effects , Seeds/drug effects , Carotenoids/metabolism , Chlorophyll/metabolism , Coriandrum/growth & development , Coriandrum/metabolism , Dose-Response Relationship, Drug , Seedlings/growth & development , Seedlings/metabolism , Seeds/growth & development , Seeds/metabolism
6.
Indian J Sex Transm Dis AIDS ; 32(1): 19-22, 2011 Jan.
Article in English | MEDLINE | ID: mdl-21799571

ABSTRACT

AIM: To study level of satisfaction among patients attending ART centre using Patient Satisfaction Questionnaire (PSQ - 18). MATERIALS AND METHODS: A cross-sectional interview based technique was used to study the level of satisfaction. RESULTS: A total of 59 patients attending ART centre were included in the study. The mean score for general satisfaction was 3.22 (SD 0.86), for technical quality 3.03 (SD 0.92), for the interpersonal manner 3.25 (SD 0.93), for communication (SD 0.90), for financial aspects was 2.38 (SD 1.00) and for time spent during the visit, the mean score was 2.97 (SD 0.98) INTERPRETATION: Patient satisfaction is the key in planning and formulating guidelines for treatment and care.

7.
Physiol Mol Biol Plants ; 15(4): 303-10, 2009 Oct.
Article in English | MEDLINE | ID: mdl-23572940

ABSTRACT

Gas exchange characteristics of thirty landraces of rice (Oryza sativa L.) along with an introduced variety VL-206 (recommended high yielding variety for rainfed uplands of the Indian Central Himalaya, ICH), grown in earthen pots and kept in the open at the Institute nursery at Kosi (1150m amsl, 79°38'10″E and 29°38'15″N) were studied. The photosynthetic rate (Pn) and other related parameters were found to vary considerably among landraces. Based on the comparison of Pn of landraces with that of VL-206, these were categorized into two groups: i) high (〉6.0 µmol m(-2)s(-1)), and ii) low (<6.0 µmol m(-2)s(-1)) Pn groups. Overall the landraces belonging to the high Pn group (Saurajubawan, Syaudhan and Taichin) which had higher stomatal conductance (gs), transpiration rate (E), water use efficiency (WUE), mesophyll efficiency (low Ci/gs ratio) and chlorophyll (Chl) content in comparison to landraces in the low Pn group. However, significant differences were not found in the intercellular CO2 concentration (Ci) between landraces belonging to the high and low Pn groups. Differences among landraces were found with regard to dark respiration; landraces with low Pn respired proportionately more of the carbon fixed than those of high Pn group. Based on the studied gas exchange characteristics Saurajubawan, Syaudhan and Taichin, local landraces of rice, may be identified as potential cultivars with high Pn and WUE.

8.
J Biol Chem ; 276(30): 28261-7, 2001 Jul 27.
Article in English | MEDLINE | ID: mdl-11375993

ABSTRACT

Osteopontin (OPN) is a secreted phosphoprotein shown to function in wound healing, inflammation, and tumor progression. Expression of OPN is often co-localized with members of the matrix metalloproteinase (MMP) family. We report that OPN is a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). Three cleavage sites were identified for MMP-3 in human OPN, and two of those sites were also cleaved by MMP-7. These include hydrolysis of the human Gly166-Leu167, Ala201-Tyr202 (MMP-3 only), and Asp210-Leu211 peptide bonds. Only the N-terminal Gly-Leu cleavage site is conserved in rat OPN (Gly151-Leu152). These sites are distinct from previously reported cleavage sites in OPN for the proteases thrombin or enterokinase. We found evidence for the predicted MMP cleavage fragments of OPN in vitro in tumor cell lines, and in vivo in remodeling tissues such as the postpartum uterus, where OPN and MMPs are co-expressed. Furthermore, cleavage of OPN by MMP-3 or MMP-7 potentiated the function of OPN as an adhesive and migratory stimulus in vitro through cell surface integrins. We predict that interaction of MMPs with OPN at tumor and wound healing sites in vivo may be a mechanism of regulation of OPN bioactivity.


Subject(s)
Matrix Metalloproteinase 3/metabolism , Matrix Metalloproteinase 7/metabolism , Sialoglycoproteins/metabolism , Amino Acids/chemistry , Animals , Binding Sites , Blotting, Western , CHO Cells , Cell Adhesion , Cell Movement , Cricetinae , Dose-Response Relationship, Drug , Female , HeLa Cells , Humans , Immunoblotting , In Situ Hybridization , Integrins/metabolism , Matrix Metalloproteinase 3/chemistry , Matrix Metalloproteinase 7/chemistry , Models, Chemical , Osteopontin , Peptides/chemistry , Protein Binding , Rats , Recombinant Proteins/metabolism , Sialoglycoproteins/chemistry , Signal Transduction , Substrate Specificity , Uterus/metabolism , Wound Healing
9.
N Z Med J ; 101(841): 122, 1988 Mar 09.
Article in English | MEDLINE | ID: mdl-3132664
SELECTION OF CITATIONS
SEARCH DETAIL