ABSTRACT
The herpes simplex virus, also known as HSV, is an important human pathogen. Acyclovir (ACV) is the first-line antiviral for the treatment of HSV infections; nevertheless HSV resistance to ACV has been increasingly reported and, therefore, search for alternative drugs have been encouraged. Herein, the effect of Cucumis melo sulfated pectin (SPCm) was evaluated in the HSV-1 infection. Pectin cytotoxicity and its antiherpetic action were determined by assays of MTT and plaque reduction, respectively. The SPCm concentration that reduced the cell viability by 50% (CC50) was 1440 µg/mL, while the concentration that reduced PFU in 50% (IC50) was 6 µg/mL against ACV-sensitive (KOS) strain and 12 µg/mL for ACV-resistant (AR-29) strain. The pectin showed high selectivity index (SI) for both viral strains. Therefore, we suggest that SPCm has been effective for HSV-1, strenghten by viral protein and DNA syntheses inhibition. In conclusion, we have found that SPCm is a promising alternative compound to control HSV infection.
Subject(s)
Antiviral Agents/pharmacology , Cucumis melo/chemistry , Herpesvirus 1, Human/drug effects , Pectins/pharmacology , Acyclovir/pharmacology , Animals , Antiviral Agents/isolation & purification , Chlorocebus aethiops , Drug Resistance, Viral/drug effects , Herpes Simplex/virology , Inhibitory Concentration 50 , Pectins/isolation & purification , Vero Cells , Virus Replication/drug effectsABSTRACT
Mangiferin is found in many plant species as the mango tree (Mangifera indica) with ethnopharmacological applications and scientific evidence. The emergence of resistant herpes simplex virus (HSV) strains to Acyclovir (ACV) has encouraged the search for new drugs. We investigated the in vitro and in vivo activity of mangiferin obtained from M. indica against ACV-resistant HSV-1 (AR-29) and sensitive (KOS) strains. The in vitro activity was performed under varying treatment protocols. The substance showed a CC50 > 500 µg/mL and IC50 of 2.9 µg/mL and 3.5 µg/mL, respectively, for the AR-29 and KOS strains. The in vivo activity was performed in Balb/c mice treated with 0.7% topical mangiferin formulation. This formulation inhibited most effectively the AR-29 strain, attenuated the lesions, postponed their appearance or enhanced healing, in comparison to control group. We demonstrated the potentiality of mangiferin from M. indica to control HSV replication with emphasis to ACV-resistant infection.
Subject(s)
Acyclovir/pharmacology , Antiviral Agents/pharmacology , Herpes Simplex/drug therapy , Herpesvirus 1, Human/drug effects , Mangifera/chemistry , Xanthones/pharmacology , Acyclovir/chemistry , Animals , Antiviral Agents/chemistry , Antiviral Agents/isolation & purification , Cells, Cultured , Chlorocebus aethiops , Dose-Response Relationship, Drug , Drug Resistance, Viral/drug effects , Microbial Sensitivity Tests , Molecular Structure , Structure-Activity Relationship , Vero Cells , Xanthones/chemistry , Xanthones/isolation & purificationABSTRACT
Adenanthera pavonina is a native tree of Africa and Asia, introduced in Brazil for reforestation and wood industry. Several pharmacological activities have described scientifically, including antiviral activity. This study evaluated the antiviral effect of sulfated polysaccharide of Adenanthera pavonina (SPAp) against acyclovir (ACV)-resistant (AR-29) and sensitive (KOS) herpes simplex virus strains. The 50% cytotoxic concentration (CC50) was determined by MTT method and the 50% inhibitory concentration (IC50) was evaluated by plaque reduction assay. The in vivo SPAp antiviral activity was performed in Balb/c mice infected by skin scarification and treated with topical 0.5% (w/w) SPAp formulations. SPAp showed a CC50 of 47.81 µg/mL and the IC50 were 0.49 µg/mL (SI = 97.5) and 0.54 µg/mL (SI = 88.5) for the strains KOS and AR-29, respectively. Our results demonstrated that mice treated with SPAp presented a delay in the development and progression of skin lesions compared with the control group.
