ABSTRACT
Prismatic silver nanoparticles (PNps) were used in the surface enhanced Raman scattering (SERS) study of the antioxidant alkaloid boldine (5,6,6a,7-tetrahydro-1,10-dimethoxy-6-methyl-4H-dibenzo[de,g]quinoline-2,9-diol). Prismatic and quasi-spherical (QsNps) silver nanoparticles were synthesized and characterized by UV-Vis spectra, topographic profile (AFM) and zeta potential measurements. Raman and infrared (IR) spectra of the boldine were registered. Theoretical model calculations of the boldine onto the Ag surface predict a nearly coplanar orientation of the benzo[de]quinoline moiety and non-bonded interactions (electrostatic).
Subject(s)
Antioxidants/chemistry , Aporphines/chemistry , Metal Nanoparticles/chemistry , Silver/chemistry , Spectrum Analysis, Raman/methods , Models, MolecularABSTRACT
Raman and surface-enhanced Raman scattering (SERS) spectra of the synthetic carboxy terminal peptide of human chorionic gonadatropin ß-subunit free of carbohydrate moieties(P37) are reported. The spectral analysis is performed on the basis of our reported Raman spectrum and SERS data of oligopeptides displaying selected amino acids sequences MRKDV, ADEDRDA, and LGRGISL. SERS samples of P37 were prepared by coating the solid peptide with metal colloids on a quartz slide. This treatment makes possible to obtain high spectral batch to batch reproducibility. Amino acids components of P37 display net charges and hydrophobic characteristics, which are related to particular structural aspects of the adsorbate-substrate interaction. The spectroscopic results are supported by quantum chemical calculations performed by using extended Hückel theory method for a model of P37 interacting with an Ag surface. The P37-metal interaction is drove by positively charged fragments of selected amino acids,mainly threonine 109, lysine 122, and arginine in positions 114 and 133. Data here reported intend to contribute to the knowledge about the antigen-antibody interaction and to the drugs delivery research area
Subject(s)
Chorionic Gonadotropin, beta Subunit, Human/chemistry , Amino Acid Sequence , Chorionic Gonadotropin, beta Subunit, Human/genetics , Humans , Hydrophobic and Hydrophilic Interactions , In Vitro Techniques , Metal Nanoparticles , Models, Molecular , Oligopeptides/chemistry , Oligopeptides/genetics , Peptide Fragments/chemistry , Peptide Fragments/genetics , Spectrum Analysis, Raman/methods , Static ElectricityABSTRACT
Arginine amino acid (Arg) has been vibrationally studied through its infrared, Raman and surface-enhanced Raman scattering (SERS) spectra, and theoretical calculations. Net charge is used to predict the possibility to obtain the SERS spectrum of Arg in colloidal solution. The interpretation of the SERS spectral data suggests that the Arg-Ag nanoparticles interaction in a colloidal solution and in the case of the Arg coated by Ag is mainly verified through the guanidinium moiety. Theoretical calculations performed by using extended Hückel theory method for a model of Arg interacting with an Ag cluster support the observed SERS experimental result.
Subject(s)
Arginine/chemistry , Spectrum Analysis, Raman/methods , Metal Nanoparticles/chemistry , Models, Molecular , Models, Theoretical , Molecular Structure , Silver/chemistryABSTRACT
BACKGROUND: The health care network of a University in Chile started a family based primary health care system using pre paid model as financing. AIM: To describe the results of this family Health Care Center. MATERIAL AND METHODS: Between 1999 and 2006, the Pontificia Universidad Catolica-Health Network offered a family care plan characterized by a preferential access to a Family Care Center (CSF), that coordinated and solved all health care needs. Several indicators and perception of satisfaction were used to assess the results of this plan, in 2003 and 2004. RESULTS: During the study period, 5,402 people were attended. Seventy three percent of these were aged between 15-44 years. The waiting lapse was less than 48 hours for medical appointments and less than 30 days for programmed surgical procedures. The coverage of preventive actions varied from 26% of mammographies among women between 25 to 69 years old to 100% for healthy child care among newborns. Thirty one percent of diabetics achieved a glycosilated hemoglobin of less than 7% and 19% of hypertensive subjects achieved blood pressure values below 135/85 mmHg. Family physicians had 2.3 appointments per person per year and solved 85% of the medical problems that motivated the consultation. In average, 88% of interviewed subjects evaluated the attention received as very good or excellent. CONCLUSIONS: The application of this model in the private sector achieves measurable health care results with a good degree of client satisfaction.
Subject(s)
Delivery of Health Care/statistics & numerical data , Family Health , Primary Health Care/statistics & numerical data , Private Sector , Quality of Health Care , Adolescent , Adult , Age Distribution , Aged , Child , Child, Preschool , Chile , Delivery of Health Care/organization & administration , Delivery of Health Care, Integrated , Female , Health Care Surveys , Health Services Accessibility , Humans , Infant , Infant, Newborn , Male , Middle Aged , Patient Satisfaction , Primary Health Care/organization & administration , Quality Indicators, Health Care , Referral and Consultation/statistics & numerical data , Sex Distribution , Young AdultABSTRACT
Background: The health care network of a University in Chile started a family based primary health care system using pre paid model as financing. Aim: To describe the results of this family Health Care Center. Material and Methods: Between 1999 and 2006, the Pontificia Universidad Catolica-Health Network offered a family care plan characterized by a preferential access to a Family Care Center (CSF), that coordinated and solved all health care needs. Several indicators and perception of satisfaction were used to assess the results of this plan, in 2003 and 2004. Results: During the study períod, 5,402 people were attended. Seventy three percent of these were aged between 15-44 years. The waiting lapse was less than 48 hours for medical appointments and less than 30 days for programmed surgical procedures. The coverage of preventive actions varied from 26 percent of mammographies among women between 25 to 69 years old to 100 percent for healthy child care among newborns. Thirty one percent of diabetics achieved a glycosilated hemoglobin of less than 7 percent and 19 percent of hypertensive subjects achieved blood pressure values below 135/85 mmHg. Family physicians had 2.3 appointments per person per year and solved 85 percent of the medicalproblems that motivated the consultation. In average, 88 percent of interviewed subjects evaluated the attention received as very good or excellent. Conclusions: The application of this model in the private sector achieves measurable health care results with a good degree ofclient satisfaction.
Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Young Adult , Delivery of Health Care/statistics & numerical data , Family Health , Primary Health Care/statistics & numerical data , Private Sector , Quality of Health Care , Age Distribution , Chile , Delivery of Health Care, Integrated , Delivery of Health Care/organization & administration , Health Care Surveys , Health Services Accessibility , Patient Satisfaction , Primary Health Care/organization & administration , Quality Indicators, Health Care , Referral and Consultation/statistics & numerical data , Sex Distribution , Young AdultABSTRACT
El recién nacido presenta con frecuencia infecciones bacterianas severas que pueden o no acompañarse de neutropenia, las que se ven favorecidas por una insuficiencia cualitativa y cuantitativa del sistema fagocítico neutrófilo en esta etapa de la vida. Lo anterior se acentúa aún más en el prematuro, lo que contribuye a altas tasas de morbimortalidad por infección. Publicaciones recientes sugieren la utilidad del factor estimulante de colonias granulocíticas (rH-GCF). El objetivo de nuestro estudio fue la evaluación prospectiva del uso precoz de rH-GCSF en prevención de sepsis nosocomial. Pacientes y métodos: se estudiaron 68 recién nacidos pretérmino de menos de 32 semanas de edad gestacional y peso inferior a 1.500 g. libres de infección clínica. 34 de ellos fueron asignados a tratamiento diario por un período de 15 días con rH-GCSF desde antes de las 72 horas de vida; la asignación se realizó en forma ciega y randomizada por tablas de números al azar. Resultados: la morbilidad infecciosa tanto sospechada como confirmada no mostró diferencias entre ambos grupos, a pesar de lograr una neutrofilia significativa. Conclusión: en este estudio, rH-GCF no demostró ser útil en la prevención de sepsis de origen intrahospitalario
Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Granulocyte Colony-Stimulating Factor , Cross Infection/prevention & control , Double-Blind Method , Granulocyte Colony-Stimulating Factor , Infant, Premature , Cross Infection/etiology , SepsisABSTRACT
Background: Anemia is common among very low birth weight newborns and requires frequent blood tranfusions. Erythropoietin was been reported to be useful in the prevention of this anemia. Aim: To asses the benefits of early (before the third week of life) Human recombinant Erythropoietin (r-EPO) administration to reduce the requirement of blood tranfusions in very low birth weight newborns. Patients and methods: sixty newborns under 1500g of birthweight were randomly assigned to recive r-EPO (n=29) or placebo (n=31) three times per week, during four weeks. Packed red cell volume and reticulocyte counts were measured weekly. Serum erythropoietin was measured prior to eigth dose. Transfusion requirements were recorded. Results: r-EPO reduced transfusions from 1.41 ñ 1.1 to 0.69 ñ 1 transfusions/newborns (p<0.001). At the fourth week of treatment, reticulocyte count was 14.8 ñ 7 and 6.4 ñ 4.9 percent in the active treatment group and placebo group respectively (p<0.001). Conclusions: r-EPO reduces the requrement of transfusions in low birth weight infants
Subject(s)
Humans , Male , Female , Infant, Newborn , Erythropoietin/pharmacology , Infant, Premature, Diseases/drug therapy , Anemia, Neonatal/prevention & control , Infant, Premature, Diseases/prevention & control , Anemia, Neonatal/drug therapy , Double-Blind Method , Infant, Low Birth Weight , Blood TransfusionABSTRACT
Se exponen los casos de 62 pacientes infértiles sometidas a salpingolisis y/u ooforolisis secundarias a proceso, con el objetivo de conocer sus resultados y establecer un pronóstico. El 40,3 por ciento se manejó por laparotomía, mientras que el resto fue intervenido por vía laparoscópica, sin diferencias en los resultados. El promedio de edad del grupo 33,3 años con un rango de edad que fluctúa entre 22 y 44 años. Dentro de los antecedentes destacan: uso de DIU 29 por ciento, cirugía pélvica previa 56,4 por ciento, endometriosis 12,9 por ciento, sapingectomía por tubario previo 9,7 por ciento y otro factor de infertilidad asociado en un 27,4 por ciento. Se realizó cirugía bilateral en 56,5 por ciento y unilateral en el 43,5 por ciento. La tasa global de embarazos obtenida fue del 32,3 por ciento (embarazos a términos 24,2 por ciento, abortos 4,8 por ciento y embarazos ectópico 3,2 por ciento). Sólo el antecedente de gestación ectópica previa fue de mal pronostico para embarazo, no existiendo diferencias para otros datos analizados. Creemos que las técnicas microquirúrgicas siguen siendo la primera aproximación terapéutica para el manejo de la infertilidad por factor tuboperitoneal, usando preferentemente la cirugía laparoscópica
