Evaluation of Viability of Periodontal Ligament Cells using Propolis, Coconut Water, Aleo Vera, and Soy Milk: An In vitro Comparative Study.

Objectives: This research was done to assess the periodontal ligament cells viability using propolis, coconut water, aloe vera, and soy milk storage media. Materials and Methods: Periodontal ligament cells were taken from freshly extracted teeth and stored into four groups of storage media: Group A-propolis, Group B-coconut water, Group C-aloe vera, and Group D-soy milk. Later, the cell viability was assessed at 1, 12, and 24 hours. Results: The periodontal ligament cell viability was significantly greater in propolis, followed by coconut water, aloe vera, and soy milk. Conclusion: We found that propolis, coconut water, aloe vera, and soy milk can be used as an effective storage media. These are naturally occurring and easily available storage medium.

Efficacy of Ozone to Eliminate Endopathogenic Microorganism in Rootcanal Biofilm.

Objective: The researchers wanted to see whether ozonated water with ultrasonication and sodium hypochlorite can destroy Enterococcus faecalis bacteria in root canals. Materials and Methods: A total of 40 single-rooted human teeth were used. A total of 100 roots were harvested and mechanically prepared. The root canals were randomly divided into four classes (n = 10) after being infected with E. faecalis for 24 h. Each sample's MTT value was calculated. Conclusions: NaOCl and aqueous ozone provide antibacterial effects in in-vitro conditions in root canals.