Synthesis and characterization of Se doped Fe3O4 nanoparticles for catalytic and biological properties.

In this study, Se-doped Fe3O4 with antibacterial properties was synthesized using by a coprecipitation method. The chemistry and morphology of the Se doped Fe3O4 nanocomposite were characterized by energy-dispersive X-ray spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, vibrating sample magnetometry, and Brunauer-Emmett-Teller spectroscopy. The antibacterial activity of the Fe3O4/Se nanocomposite was examined against G+ (Gram-positive) and G- (Gram-negative) bacteria, in the order Staphylococcus aureus, Staphylococcus saprophyticus, Pseudomonas aeruginosa, Klebsiella pneumonia, and Escherichia coli, which are the most harmful and dangerous bacteria. Fe3O4/Se, as a heterogeneous catalyst, was successfully applied to the synthesis of pyrazolopyridine and its derivatives via a one-pot four-component reaction of ethyl acetoacetate, hydrazine hydrate, ammonium acetate, and various aromatic aldehydes. Fe3O4/Se was easily separated from the bacteria-containing solution using a magnet. Its admissible magnetic properties, crystalline structure, antibacterial activity, mild reaction conditions, and green synthesis are specific features that have led to the recommendation of the use of Fe3O4/Se in the water treatment field and medical applications. Direct Se doping of Fe3O4 was successfully realized without additional complicated procedures.

Synergies in antimicrobial treatment by a levofloxacin-loaded halloysite and gold nanoparticles with a conjugation to a cell-penetrating peptide.

Herein, a novel designed antimicrobial therapeutic drug delivery system is presented, in which halloysite nanotubes (HNTs) encapsulate a determined dosage of levofloxacin (lvx). Moreover, gold nanoparticles (AuNPs) have been embedded into the structure for plasmonic heating under irradiation of the green LED light (7 W, 526 nm). It was revealed that the plasmonic heating of the AuNPs leads to a controlled trend in the lvx release process. Also, a synergistic effect on the antimicrobial activity of the prepared therapeutic system has been observed through photothermal heating of the structure. To enhance the cell adhesion, a cell-penetrating peptide sequence (CPP) is conjugated to the surfaces. This CPP has led to quick co-localization of the prepared nano-cargo (denoted as lvx@HNT/Au-CPP) with the bacterial living cells and further attachment (confirmed by confocal microscopy). Concisely, the structure of the designed nano-cargo has been investigated by various methods, and the in vitro cellular experiments (zone of inhibition and colony-counting) have disclosed that the antimicrobial activity of the lvx is significantly enhanced through incorporation into the HNT/Au-CPP delivery system (drug content: 16 wt%), in comparison with the individual lvx with the same dosage. Hence, it can be stated that the bacterial resistance against antibiotics and the toxic effects of the chemical medications are reduced through the application of the presented strategy.

Synthesis of Cu(OH)2 nanowires modified by Fe3O4@SiO2 nanocomposite via green and innovative method with antibacterial activity and investigation of magnetic behaviours.

In this study, green synthesis of modified Cu(OH)2 nanowires by Fe3O4@SiO2 core-shell nanospheres was easily performed via chemical reduction. In other words, the direct coating of Cu(OH)2 on Fe3O4@SiO2 was successfully realized without the extra complicated procedures. Various concentrations of synthesized nanocomposites were tested on pathogenic and nosocomial bacteria. In this study, the structural information and characterization of Fe3O4@SiO2/Cu(OH)2 nanowires (FSCNWs) were obtained using FE-SEM, FT-IR, EDX and X-ray diffraction. This nanocomposite can effectively kill important infectious bacteria, including Staphylococcus aureus, Escherichia coli, Staphylococcus saprophyticus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Studies have shown that FSCNW nanocomposites affect common antibiotic-resistant bacteria. This result confirms the function of FSCNW as an effective, beneficial and environmentally friendly antibacterial agent that can used in a wide range of applications in medicine. FSCNWs can be separated conveniently from bacteria-containing solutions using a magnet. Compared with nanocomposites based on other metals such as silver and gold, the use of FSCNWs in water treatment has been recommended because of the precursor of copper for its low price and less toxicity. In addition to its special properties such as mild reaction conditions, green synthesis methods, admissible magnetic properties, easy separation, high antibacterial activity and beneficial efficiency.

Design, synthesis, and characterization of novel eco-friendly chitosan-AgIO3 bionanocomposite and study its antibacterial activity.

This work reports a facile and green approach to preparing AgIO3 nanoparticles decorated with chitosan (chitosan-AgIO3). The bionanocomposite was fully characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) images, energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction analysis (XRD). The antibacterial effect of chitosan-AgIO3 bionanocomposite was investigated for Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus saprophyticus, Escherichia coli, and Staphylococcus aureus as pathogen microorganisms via the plate count method, disk diffusion method, and optical density (OD) measurements. The antibacterial performance of the bionanocomposite was compared with two commercial drugs (penicillin and silver sulfadiazine) and in some cases, the synthesized bionanocomposite has a better effect in the eradication of bacteria. The bionanocomposite represented great antibacterial properties. Flow cytometry was performed to investigate the mechanism of bionanocomposite as an antibacterial agent. Reactive oxygen species (ROS) production was responsible for the bactericidal mechanisms. These results demonstrate that the chitosan-AgIO3 bionanocomposite, as a kind of antibacterial material, got potential for application in a broad range of biomedical applications and water purification. The design and synthesis of green and biodegradable antibacterial materials with simple processes and by using readily available materials cause the final product to be economically affordable and could be scaled in different industries.

Assessment of catalytic and antibacterial activity of biocompatible agar supported ZnS/CuFe2O4 magnetic nanotubes.

The tubular magnetic agar supported ZnS/CuFe2O4 nanocomposite was fabricated via a simple procedure. Next, various properties of this nanocomposite were studied by employing multiple characterization techniques including FT-IR, EDX, SEM, TEM,VSM, XRD, and TGA. Then, the catalytic and antibacterial applications were evaluated for the fabricated nanocomposite. Based on the experimental result, the nanocomposite showed excellent catalytic activity to promote the multicomponent reaction between ethyl acetoacetate, hydrazine hydrate, aromatic aldehydes, and malononitrile to synthesize a variety of dihydropyrano[2,3-c]pyrazole derivatives with high yields (89-95%) in acceptable reaction times (20-40 min) under mild reaction conditions. It can be efficiently recycled and re-work in six consequent runs without notable reduction in catalytic productiveness. Furthermore, its antibacterial activity was assessed against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria by the agar diffusion and plate-count methods. These results indicate that the width of the inhibition zone around the S. aureus (G+ bacterium) is more than that of E. coli (G- bacterium). Moreover, the agar supported ZnS/CuFe2O4 nanocomposite exhibited strong prevention of the bacterial colonies' growth.

Fe3O4@chitosan-tannic acid bionanocomposite as a novel nanocatalyst for the synthesis of pyranopyrazoles.

Recently magnetic nanocatalyst has attracted considerable attention because of its unique properties, including high performance, easy separation from the reaction mixture, and recyclability. In this study, a novel magnetic bionanocomposite was synthesized with chitosan and tannic acid as a natural material. The synthesized bionanocatalyst was characterized by essential analysis. Fe3O4@chitosan-tannic acid as a heterogeneous nanocatalyst was successfully applied to synthesize pyranopyrazole and its derivatives by a one-pot four-component reaction of malononitrile, ethyl acetoacetate, hydrazine hydrate, and various aromatic aldehyde. At the end of the reaction, the nanocatalyst was separated from the reaction mixture and was reused several times with no significant decrease in its catalytic performance. Simple purification of products, the ability for recovering and reusing the nanocatalyst, eco-friendliness, high yields of pure products, mild reaction conditions, short reaction time, non-toxicity, economically affordable are some of the advantages of using the fabricated nanocatalyst in the synthesis of pyranopyrazole.

Bifunctional PVA/ZnO/AgI/Chlorophyll Nanocomposite Film: Enhanced Photocatalytic Activity for Degradation of Pollutants and Antimicrobial Property under Visible-Light Irradiation.

Here, poly(vinyl alcohol) (PVA) with numerous hydroxyl groups has been applied as a suitable substrate for efficient formation of zinc oxide (ZnO) nanoparticles with a flower shape (confirmed by electron-scanning microscopy), silver iodide (AgI) nanoparticles, and chlorophyll (Chl), as a natural-based photocatalyst (PVA/ZnO/AgI/Chl). First, an efficient preparation route for the PVA/ZnO/AgI/Chl nanophotocatalyst is presented starting from the extraction of Chl from fresh spinach. Then, the catalytic role of the prepared composite is precisely investigated in degradation of methylene blue (MB). The effects of visible-light irradiation, different contact times, and the employed ingredients on the architecture of the PVA/ZnO/AgI/Chl are screened in the degradation process of MB. It is demonstrated that the best result (MB removal efficiency ca. 95.5%) is achieved by applying the visible-light irradiation using a LED lamp (70 W, λ = 425 nm) for a 60 min duration. Moreover, the photocatalytic performance of PVA/ZnO/AgI/Chl has been further confirmed by degradation of Congo red (CR) (ca. 92%, in 150 min) and 4-chlorophenol (4-CP) (88%, in 270 min), as well. As another function of the prepared PVA/ZnO/AgI/Chl composite, a substantial antibacterial property against human bacterial pathogens such as Staphylococcus aureus and Escherichia coli as Gram-positive and Gram-negative bacteria has been noticed, studied by agar diffusion cup plate and colony methods. The zones of inhibition have been evaluated ca. 20 and 12 mm for the S. aureus and E. coli cell lines, respectively. Finally, a great synergy between the prepared composite and the visible light has been observed through the examination of the live bacteria: 99.6% for S. aureus and 99.8% for E. coli in the presence of visible light, after the subjection of PVA/ZnO/AgI/Chl particles to the bacteria, verified by the colony counter method.

Author Correction: Synthesis of nickel nanoparticles by a green and convenient method as a magnetic mirror with antibacterial activities.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Synthesis of nickel nanoparticles by a green and convenient method as a magnetic mirror with antibacterial activities.

In this work, a simple protocol was described for the synthesis of nickel magnetic mirror nanoparticles (NMMNPs) including antibacterial activities. The identification of NMNPs was carried out by field-emission scanning electron microscopy (FESEM) images, energy-dispersive X-ray (EDX) analysis, X-ray diffraction (XRD) pattern, transmission electron microscopy (TEM) images and vibrating sample magnetometer (VSM) curve. The antibacterial activities are investigated against S. aureus and E. coli as the Gram-positive and Gram-negative bacteria, respectively. The UV-Vis absorption was also studied in the present of NMMNPs at different time intervals that disclosed decreasing of the bacterial concentration. More than 80% of the bacteria were disappeared after treating in the presence of NMMNPs for 18 h. The Ni-NPs revealed an excellent mirror attribute with a well-controlled transmission (7%). A better light-reflectivity over conventional glass or a mercury mirror proved their utility for domestic uses in comparison with conventional mirrors as rather toxic materials like mercury. Owing to its magnetic properties, this kind of mirror can be easily made onto glass by using an external magnet. An ordered crystalline structure, admissible magnetic properties, substantial antibacterial activities, tunable mirror properties, mild reaction conditions, and overall, the facile synthesis are the specific features of the present protocol for the possible uses of NMMNPs in diverse applications.

Enhanced activity of vancomycin by encapsulation in hybrid magnetic nanoparticles conjugated to a cell-penetrating peptide.

We describe a novel antibiotic delivery system based on magnetic nanoparticles (NPs) conjugated to a cell-penetrating peptide (CPP). Silica-coated iron oxide NPs were produced via a co-deposition method, and coated by a polyvinyl alcohol (PVA) polymeric network via physicochemical binding. Vancomycin (VAN) was then entrapped into this PVA network. A hexapeptide sequence Gly-Ala-Phe-Pro-His-Arg, was synthesized in the solid phase and then conjugated onto the surface of the magnetic NPs. The drug ratio incorporation into the carrier system and drug release were monitored through precise analysis. Confocal microscopy showed that the NPs could be internalized into Staphylococcus aureus and Escherichia coli bacterial cells. The antimicrobial effects of VAN were significantly enhanced by this system with a low dosage of VAN. Advantages include rapid targeted-drug delivery process, drug dose reduction, and equal effects on both Gram-positive and Gram-negative bacteria.

Cu(ii) immobilized on Fe3O4@HNTs-tetrazole (CFHT) nanocomposite: synthesis, characterization, investigation of its catalytic role for the 1,3 dipolar cycloaddition reaction, and antibacterial activity.

In the present study, Cu(ii) immobilized on an Fe3O4@HNTs-tetrazole (CFHT) nanocomposite was designed and prepared. For this, halloysite nanotubes (HNTs) as natural mesoporous substances were modified during several chemical reactions. The synthesis of the CFHT nanocomposite was investigated step by step with the required physicochemical techniques such as FT-IR, EDX, SEM, TEM, XRD, VSM, TGA and CHNS analyses. After ensuring that the nanocomposite was successfully prepared, its catalytic application in the synthesis of the 5-substituted 1H-tetrazole derivatives via multicomponent reactions (MCRs) between aromatic aldehydes, malononitrile, and sodium azide was assessed. According to the experimental results, the prepared nanocomposite exhibited excellent capability for conducting this MCR reaction. All desired products were obtained in a short reaction time (30-40 min) with high productivity (90-97%) and without a complicated workup procedure. Furthermore, the magnetic property of the synthesized heterogeneous nanocomposite empowers it to be recovered and reused in five times successive reactions without any significant reduction in reaction efficiency. Moreover, the remarkable antibacterial activity of the nanocomposite against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was evaluated by agar diffusion and plate-count methods. The zones of inhibition were around 16 and 20 mm for E. coli and S. aureus bacteria, respectively. Also, colony analysis confirms the killing of bacteria by using the CFHT nanocomposite.