ABSTRACT
In this paper, the Gaussian optical properties and the first- and third-order isotropic and anisotropic chromatic aberrations of the Glaser magnetic lens have been derived analytically and then calculated numerically based on two methods: the differential-algebraic DA and the electron optical aberration integrals. These coefficients have been recalculated for object magnetic immersion OMI. The expressions of chromatic aberration coefficients with the inclusion of the OMI effect of round electron lenses were not published in the literature until the present work. The numerical results of high-order chromatic aberration coefficients of Glaser magnetic lenses calculated using the DA method are shown to be in excellent agreement with those calculated using the integral aberration method and with the minor relative errors of order (10-7- 10-8), indicating that all the formulas for the chromatic aberration coefficients are entirely correct. For this purpose, COSY INFINITY 10 and Mathematica 11 were used, and both proved to be excellent computer programs for this work.
ABSTRACT
The study was conducted to detect haptoglobin in seminal plasma (SP-Hp) of Awassi rams and the effect of the breeding season on its concentration, along with determining the correlation with its concentration in serum (S-Hp) and main semen variables. Pre-warmed artificial vagina was used to collect semen samples biweekly from five Awassi rams. Semen samples were evaluated for volume, concentration and sperm motility. Blood samples were collected 10-30 min after semen collection. The concentration of serum and seminal plasma Hp was determined using ELISA. The mean ± SESP-Hp concentrations ranged from 0.25 ± 0.05 to 0.81 ± 0.44 µg/ml, whereas those of S-Hp-ranged from 0.99 ± 0.29 to 2.99 ± 0.18 mg/ml. There was a significant (P < 0.05) positive correlation (r = 0.329) between SP-Hp and S-Hp concentrations. Both SP-Hp and S-Hp concentrations were significantly (P < 0.05) higher in winter as compared with the other seasons. The concentrations of SP-Hp and S-Hp during the breeding season were significantly lower (P < 0.01) than those of the out season period. SP-Hp concentration negatively correlated with semen volume and sperm concentration (r = -0.164 and -0.121), whereas sperm concentration positively correlated with individual sperm motility (r = 0.100). No significant correlation was detected between SP-Hp and semen parameters. It can be concluded that, Hp is present in ram seminal plasma and its concentration was about 2000 folds lower than that of the serum, and Hp concentration was lower during the breeding season, but its concentration in seminal plasma has no significant correlation with semen parameters.