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1.
Int J Mol Med ; 20(3): 345-9, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17671739

ABSTRACT

The effects of neuropeptide Y (NPY) on adrenal glucocorticoid secretion are controversial, and we have investigated this issue in guinea pigs, where, like in humans and cows, the main glucocorticoid hormone is cortisol. In vivo experiments showed that prolonged NPY administration markedly lowered cortisol plasma concentration not only in normal guinea pigs, but also in animals whose hypothalamic-pituitary-adrenal axis and renin-angiotensin system had been pharmacologically interrupted by the simultaneous administration of dexamethasone and captopril. In vitro experiments ruled out the possibility that in vivo glucocorticoid anti-secretagogue action of NPY can ensue from a direct effect on the adrenal gland. In fact, NPY did not affect cortisol secretion from dispersed guinea pig inner adrenocortical cells. In contrast, NPY raised cortisol production from adrenal slices containing medullary tissue, and this effect was blocked by the beta-adrenoceptor antagonist l-alprenolol. This finding, coupled with the demonstration that NPY enhanced catecholamine release from guinea pigadrenomedullary tissue, strongly suggests that NPY may stimulate glucocorticoid secretion in this species through an indirect mechanism involving catecholamines, that in a paracrine manner promote the secretion of inner adrenocortical cells. In light of these observations, the conclusion is drawn that the in vivo effects of NPY are mediated by mechanism(s) independent of either the suppression of the main adrenal agonists ACTH and angiotensin-II or the direct inhibition of adrenal secretion. The possibility merits an investigation into whether NPY enhances the production of peptides, which, like leptin, inhibit adrenal glucocorticoid secretion acting as circulating hormones.


Subject(s)
Adrenal Glands/drug effects , Adrenal Glands/metabolism , Glucocorticoids/metabolism , Neuropeptide Y/pharmacology , Adrenal Cortex/drug effects , Adrenal Cortex/metabolism , Adrenal Medulla/drug effects , Adrenal Medulla/metabolism , Alprenolol/pharmacology , Animals , Captopril/pharmacology , Catecholamines/metabolism , Dexamethasone/pharmacology , Guinea Pigs , Hydrocortisone/metabolism , In Vitro Techniques , Male
2.
Int J Mol Med ; 20(1): 91-5, 2007 Jul.
Article in English | MEDLINE | ID: mdl-17549394

ABSTRACT

Insulin-like growth factor (IGF)-I is a ubiquitously synthesized peptide that, along with IGF-II, acts via the IGF-R type I receptor. IGF-I and its receptor are expressed in the adrenal gland of humans and bovines, the secretion of which they seem to stimulate. As in humans and cows, the main glucocorticoid hormone secreted by guinea-pig adrenals is cortisol, and hence we have studied the adrenocortical effects of IGF-I in this species. In vivo experiments showed that prolonged IGF-I administration raised the plasma concentration of cortisol in both normal and dexamethasone/captopril-treated guinea pigs, thereby ruling out the possibility that IGF-I may act by activating the hypothalamic-pituitary-adrenal axis and the renin-angiotensin system. In vitro experiments demonstrated that IGF-I enhanced basal, but not maximally agonist [ACTH and angiotensin-II (Ang-II)]-stimulated, cortisol secretion from freshly dispersed guinea-pig inner adrenocortical cells. The IGF-I immuno-neutralization suppressed the IGF-I secretagogue effect, without altering the cortisol response to both ACTH and Ang-II. IGF-I raised cyclic-AMP and inositol triphosphate release from dispersed guinea-pig cells, and the effect was reversed by the adenylate cyclase inhibitor SQ-22536 and the phospholipase-C (PLC) inhibitor U-73122. SQ-22536, U-73122, the protein kinase (PK) A inhibitor H-89 and the PKC inhibitor calphostin-C decreased by approximately 50% the cortisol response of dispersed cells to IGF-I, and the combined exposure to SQ-22536 and U-73122 abolished it. We conclude that IGF-I stimulates glucocorticoid secretion from guinea-pig adrenocortical cells, acting via selective receptors coupled to both the adenylate cyclase/PKA- and PLC/PKC-dependent signaling cascades.


Subject(s)
Adrenal Cortex/cytology , Adrenal Cortex/physiology , Hydrocortisone/metabolism , Insulin-Like Growth Factor I/administration & dosage , Insulin-Like Growth Factor I/pharmacology , Adrenal Cortex/metabolism , Animals , Captopril/pharmacology , Cyclic AMP/analysis , Cyclic AMP/metabolism , Dexamethasone/pharmacology , Guinea Pigs , Hydrocortisone/analysis , Hydrocortisone/blood , In Vitro Techniques , Inositol Phosphates/analysis , Inositol Phosphates/metabolism , Male , Zona Fasciculata/physiology , Zona Reticularis/physiology
3.
Int J Mol Med ; 17(4): 633-6, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16525720

ABSTRACT

Atrial natriuretic peptide (ANP) is a regulatory hormone widely expressed, along with its receptors, in organs and body tissues. ANP is well known to inhibit aldosterone secretion from mammalian adrenals, but its effect on glucocorticoid-hormone production is controversial. In vivo experiments showed that prolonged ANP administration raised the plasma concentration of cortisol in both normal and dexamethasone/captopril-treated guinea pigs (i.e. in animals with pharmacologically interrupted hypothalamic-pituitary-adrenal axis and renin-angiotensin system). ANP did not affect cortisol secretion from dispersed guinea pig zona fasciculata-reticularis cells, but enhanced catecholamine release from adrenomedullary cells. ANP stimulated cortisol output from guinea pig adrenal slices containing medullary chromaffin tissue, and the beta-adrenoceptor antagonist l-alprenolol blocked this effect. The conclusion is drawn that ANP, when the structural integrity of the adrenal gland is preserved, is able to enhance glucocorticoid secretion in guinea pigs, through an indirect mechanism involving the rise in the catecholamine release, which in turn, acting in a paracrine manner, stimulate secretion of inner adrenocortical cells.


Subject(s)
Adrenal Glands/drug effects , Atrial Natriuretic Factor/pharmacology , Hydrocortisone/metabolism , Adrenal Glands/metabolism , Adrenal Medulla/drug effects , Adrenal Medulla/metabolism , Alprenolol/administration & dosage , Alprenolol/pharmacology , Angiotensin-Converting Enzyme Inhibitors/administration & dosage , Angiotensin-Converting Enzyme Inhibitors/pharmacology , Animals , Atrial Natriuretic Factor/administration & dosage , Captopril/administration & dosage , Captopril/pharmacology , Catecholamines/metabolism , Dexamethasone/administration & dosage , Dexamethasone/pharmacology , Dose-Response Relationship, Drug , Epinephrine/metabolism , Female , Glucocorticoids/administration & dosage , Glucocorticoids/pharmacology , Guinea Pigs , Hydrocortisone/blood , In Vitro Techniques , Injections, Subcutaneous , Male , Norepinephrine/metabolism , Paracrine Communication/drug effects , Paracrine Communication/physiology
4.
Indian J Exp Biol ; 44(1): 83-5, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16430097

ABSTRACT

The present study was undertaken to assess the toxicity of acid alone and two different sublethal concentrations of aluminium, (25% and 75% dose of 96 hr LC50 value in acidified soft water of pH 5) on red blood cells of a stenohaline catfish, C. batrachus for an acute exposure of 5 days. The scanning electron microscopic studies on all the three treated groups revealed several kinds of erythrocyte alterations and modifications with abnormal morphology. These included abnormal surface-wrinkling accompanied with excessive roughness on the membrane, erythrocytes with surface granulation in higher dose and finally the appearance of morphologically abnormal forms, the codocyte (target cell) and the stomatocyte. The results suggest that abnormality in the shape of erythrocytes could be linked to altered surface membrane area to volume ratio, decrease in cytoplasmic volume owing to reduced Hb content or increase in the amount of water content within the cell resulting from osmotic disequilibrium. In this context, the abnormal surface membrane morphology could be attributed to cytoskeleton fragility and defects in structural proteins. Further, the acid group exhibited a striking behavior of cellular adhesion and bonding to adjoining cell surfaces, culminating in several bunches which thereby reduces the surface area for gaseous exchange and could produce blocking effect while flowing through microcirculation.


Subject(s)
Aluminum/toxicity , Catfishes/blood , Erythrocytes, Abnormal/drug effects , Animals , Erythrocytes, Abnormal/ultrastructure , Hydrogen-Ion Concentration , Microscopy, Electron, Scanning
5.
J Biosci ; 27(5): 509-13, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12381875

ABSTRACT

The ultrastructure of the corpuscles of Stannius (CS) of Heteropneustes fossilis reveals a homogenous cellular composition characterized by only one cell type, with large secretory granules and abundant ribosomal endoplasmic reticulum. These cells are comparable to the type 1 cell described in the CS of other teleosts; type 2 cells, whose presence is ubiquitous in the CS of freshwater species are absent in H. fossilis. Our data on the CS of H. fossilis demonstrate that not all freshwater species possess type 2 cells in their CS and these are not essential for life in freshwater


Subject(s)
Catfishes/anatomy & histology , Endocrine Glands/ultrastructure , Animals , Microscopy, Electron
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