ABSTRACT
Members of the genus Brevibacillus belonging to the familyPaenibacillaceae are Gram-positive/variable, endospore-forming, and rod-shaped bacteria that dwell in various environmental habitats. Brevibacillus spp. have a wide range of enzyme activities such as degradation of various carbohydrates, plastics, and they possess resistance against heavy metals. These characteristics make them encouraging contenders for biotechnological applications.In this work, we analyzed the reference genomes of 19Brevibacillusspecies, focusing on discovering the biodegradation and heavy metal resistance capabilities of this little studied genus from genomic data. The results indicate that several strain specific traits were identified. For example Brevibacillus halotolerans s-14, and Brevibacillus laterosporus DSM 25 have more glycoside hydrolases (GHs) compared to other carbohydrate-active enzymes, and therefore might be more suitable for biodegradation of carbohydrates. In contrast, strains such as Brevibacillus antibioticus TGS2-1, with a higher number of glycosyltransfereases (GTs) may aid in the biosynthesis of complex carbohydrates. Our results also suggest some correlation between heavy metal resistance and polyurethane degradation, thus indicating that heavy metal resistance strains (e.g. Brevibacillus reuszeri J31TS6) can be a promising source of enzymes for polyurethane degradation. These strain specific features make the members of this bacterial group potential candidates for further investigations with industrial implications. This work also represents the first exhaustive study of Brevibacillus at the genome scale.
Subject(s)
Brevibacillus , Metals, Heavy , Biodegradation, Environmental , Brevibacillus/genetics , Brevibacillus/metabolism , Carbohydrates , DNA, Bacterial/genetics , Glycoside Hydrolases/genetics , Glycoside Hydrolases/metabolism , Metals, Heavy/metabolism , Phylogeny , Polyurethanes/metabolism , Sequence Analysis, DNA , Soil MicrobiologyABSTRACT
Acne vulgaris is the most common skin diseases that people experience during their lives. Thirteen rhizosphere isolates were screened against Propionibacterium acnes. The bacterium exhibited the highest activity against P. acnes was identified as Bacillus methylotrophicus YML008 by 16S rRNA gene sequencing. Scanning electron microscopy was used to assess the changes in morphology of P. acnes. Preliminary studies on the antimicrobial substance demonstrated the hydrophilic nature of compound with MIC of 0.17mg/ml and MBC of 0.3mg/ml. The cytotoxic effect of the extract was least (80% survival) as compared to benzyperoxide (40% survival). These results suggest YML008 as a promising bioresource and may be useful as a lead bacterium to develop a new type of anti-acne skin care prep to cure or prevent acne. Further, mechanism of action and proper clinical trials may be promising for this research.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacillus/metabolism , Dermatologic Agents/pharmacology , Propionibacterium acnes/drug effects , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/metabolism , Antibiosis , Bacillus/classification , Bacillus/genetics , Benzoyl Peroxide/pharmacology , Dermatologic Agents/isolation & purification , Dermatologic Agents/metabolism , Disk Diffusion Antimicrobial Tests , Dose-Response Relationship, Drug , Hydrophobic and Hydrophilic Interactions , Microscopy, Electron, Scanning , Propionibacterium acnes/pathogenicity , Propionibacterium acnes/ultrastructure , Ribotyping , Time FactorsABSTRACT
The biological control efficacy of a greenhouse soil bacterial mixture of Lactobacillus farraginis, Bacillus cereus, and Bacillus thuringiensis strains with antinematode activity was evaluated against the root-knot nematode Meloidogyne incognita. Two control groups planted in soil drenched with sterile distilled water or treated with the broadspectrum carbamate pesticide carbofuran were used for comparison. The results suggest that the bacterial mixture is effective as a biocontrol agent against the root-knot nematode.
