ABSTRACT
Objectives: There is limited knowledge on the efficacy of universal adhesives when used in different etching modes. The aim of this study was to evaluate the microtensile bond strength (µTBS) to dentin using universal adhesives with light-cure and dual-cure composites. Materials and Methods: In this in vitro experimental study, the occlusal third of 60 caries-free human molars were removed and the exposed surfaces were treated and allocated to 10 groups (N=6). Two different bonding agents (Single Bond Universal, G-Premio) were used to bond 2 different composite resins (Z250 light-cure and CoreFlo DC dual-cure) to dentin, using etch-and-rinse (E&R) and self-etch (SE) modes. Single Bond 2 was applied in E&R mode on wet dentin to serve as control. The specimens were sectioned into 1×1 mm2 sticks with a precision saw. A microtensile testing machine was used to measure the µTBS of the specimens with a crosshead speed of 0.5 mm/min. Data were analyzed using three-way ANOVA and Bonferroni post-hoc test (α=0.05). Results: The Single Bond Universal group in SE mode with light-cure composite yielded the highest (39.24MPa), and the G-Premio SE group with dual-cure composite produced the lowest (13.65MPa) mean µTBS (P<0.05). The dominant failure mode was adhesive, in all groups. Conclusion: The bonding agent had a significant effect on µTBS of composite to dentin, but different composites and different etching modes were not significantly different in this respect.
ABSTRACT
Drosophila suzukii is an emerging global pest of soft fruit; although it likely overwinters as an adult, larval cold tolerance is important both for determining performance during spring and autumn, and for the development of temperature-based control methods aimed at larvae. We examined the low temperature biology of third instar feeding and wandering larvae in and out of food. We induced phenotypic plasticity of thermal biology by rearing under short days and fluctuating temperatures (5.5-19°C). Rearing under fluctuating temperatures led to much slower development (42.1days egg-adult) compared to control conditions (constant 21.5°C; 15.7days), and yielded larger adults of both sexes. D. suzukii larvae were chill-susceptible, being killed by low temperatures not associated with freezing, and freezing survival was not improved when ice formation was inoculated externally via food or silver iodide. Feeding larvae were more cold tolerant than wandering larvae, especially after rearing under fluctuating temperatures, and rearing under fluctuating temperatures improved survival of prolonged cold (0°C) to beyond 72h in both larval stages. There was no evidence that acute cold tolerance could be improved by rapid cold-hardening. We conclude that D. suzukii has the capacity to develop at low temperatures under fluctuating temperatures, but that they have limited cold tolerance. However, phenotypic plasticity of prolonged cold tolerance must be taken into account when developing low temperature treatments for sanitation of this species.
