Efficacy of spongy xenogeneic scaffold loaded with simvastatin in the treatment of severe alveolar horizontal defect: A clinical and histological study.

Background: Bone tissue engineering offers several advantages for repairing skeletal defects. In this study, we designed and fabricated a scaffold for bone tissue engineering in patients with horizontal alveolar defect. Aim: The items included in the fabrication of the scaffold were xenogenic bone graft, gelatin as a substrate to improve the physical integrity of scaffold, and simvastatin to stimulate osteogenesis (10 mg per 1 g of xenograft). Methods: Fourteen patients with a horizontal defect in the alveolar ridge were enrolled in the study. Seven patients underwent routinely guided bone regeneration (GBR) using xenogenic bone graft plus collagenous membrane, and seven patients were treated with the scaffolds. After four months of follow-up after surgery, both the scaffold and GBR groups were examined for changes in the width of alveolar ridge and histologically for the quantity of newly produced bone. Results: The newly designed scaffold showed superior osteoconduction characteristics to routine GBR materials, which were used in this study. The difference in the quantity of the newly produced bone between the scaffold group and GBR group was significant and higher for the scaffold group. Regarding newly produced bone percentage, the scaffold group showed a mean of 20.93 and the GBR group presented a mean of 13.25% (P = 0.004). Also, the mean value for the duration of surgery for GBR was 45 minutes and for scaffold was 22 minutes, which was significantly lower in the scaffold group (P < 0.001). Conclusions: The newly designed scaffold is a suitable treatment modality for bone tissue engineering.

THE ASSOCIATION OF PLASMA LEVELS OF miR-34a AND miR-149 WITH OBESITY AND INSULIN RESISTANCE IN OBESE CHILDREN AND ADOLESCENTS.

CONTEXT: MicroRNAs (miRNAs) are short noncoding RNAs involved in posttranscriptional regulation of gene expression that influence various cellular functions including glucose and lipid metabolism and adipocyte differentiation. OBJECTIVE: The aim of this study was to evaluate the levels of miR-34a and miR-149 and their relationship with metabolic parameters in obese children and adolescents. DESIGN: Seventy children and adolescents were enrolled in the study. Plasma levels of microRNAs were evaluated by real-time PCR using SYBR green and analyzed by ΔCt method. Plasma concentrations of visfatin and insulin were measured by ELISA method. Glucose and lipid profile were determined colorimetrically. HOMA-IR was calculated and used as an index of insulin resistance (IR). RESULTS: miR-34a was significantly lower in subjects with insulin resistance compared to obese children with normal insulin sensitivity. There was an inverse relationship between miR-34a levels and both insulin and HOMA-IR. On the other hand, miR-149 was significantly correlated with visfatin. There was no significant difference in miR-34a and miR-149 between obese and normal weight subjects. CONCLUSIONS: miR-34a is associated with insulin and HOMA-IR and thus seems to be involved in IR. miR-149 is inversely associated with visfatin levels which could be indicative of anti-inflammatory effect of this miRNA.

Efficacy of accelerated hydrogen peroxide® disinfectant on foot-and-mouth disease virus, swine vesicular disease virus and Senecavirus A.

AIMS: In a laboratory, disinfectants used to inactivate pathogens on contaminated surfaces and to prevent spread of diseases often have adverse side effects on personnel and the environment. It is, therefore, essential to find safer, fast-acting and yet effective disinfectants. The objective of this study was to evaluate an accelerated hydrogen peroxide® (AHP® )-based disinfectant against high consequence foreign animal disease pathogens such as foot-and-mouth disease virus (FMDV) and swine vesicular disease virus (SVDV), as well as Senecavirus A (SVA), which causes similar lesions as FMDV and SVDV. METHODS AND RESULTS: We tested varying dilutions and contact times of AHP against FMDV, SVDV and SVA by the standard US EPA and modified methods. AHP was effective against all three viruses, albeit at a higher concentration and double the manufacturer recommended contact time when testing wet films of SVDV. CONCLUSIONS: AHP is an effective disinfectant against FMDV, SVDV and SVA. SIGNIFICANCE AND IMPACT OF THE STUDY: AHP-based disinfectant can, therefore, be used in high containment laboratories working with FMDV, SVDV and related pathogens.

Assessment of helicobacter pylori prevalence by scorpion real-time PCR in chronic tonsillitis patients.

BACKGROUND: Occasionally, bacteria or viruses enter the tonsils and these organs become overwhelmed by bacterial or viral infection leading to inflammation. Some studies confirmed the presence of Helicobacter pylori in tonsillar specimens of patients suffering from chronic tonsillitis and some others did not. The difference in results in various studies might be due to different laboratory methods. The aim of this study was to investigate the presence of H. pylori Deoxynucleic acid (DNA) in archival tonsillar tissues of patients with chronic tonsillitis by a rapid, sensitive, and specific technique of Scorpion real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: Scorpion real-time PCR and modified McMullen's staining was performed on 103 archival paraffin-embedded tonsillar samples collected from patients with chronic tonsillitis following tonsillectomy operation. RESULTS: Our findings showed that H Cell and Molecular Research Center. pylori DNA was present in 21.35% of total specimens by using Scorpion real-time PCR. Modified McMullen's staining of paraffin-embedded sections was positive in 19 patients. Out of our 103 samples, 50 samples showed positive a rapid urease test whereas 53 samples demonstrated negative results, 20 produced positive PCR results, and 83 were negative for H. pylori. There was no significant relationship between the presence of H. pylori, sex, age, and place of residence. CONCLUSION: Although the existence of H. pylori in tonsillar tissue samples of patients with chronic tonsillitis is controversial, however, our results showed that in our studied specimens, a significant number of patients with chronic tonsillitis had H. pylori colonization.