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1.
APMIS ; 118(1): 37-44, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20041869

ABSTRACT

The present study was designed to investigate the prevalence of herpes simplex virus type-2 (HSV-2) in Egyptian patients with bladder cancer or cystitis and to evaluate the performance of different diagnostic HSV-2 assays. The study included 50 patients: 27 with bladder cancer (group I), 23 with cystitis (group II) and 20 subjects as controls (group III). HSV-2 DNA was detected using polymerase chain reaction (PCR) on bladder tissue and buffy coat cells (BCC). Electron microscopic studies (EMS) on BCC and ELISAs for IgM, IgG and specific glycoprotein G-2 (gG-2) IgG were performed. HSV-2 DNA was detected by PCR on bladder tissue biopsies in 29.6% and 21.7% of group I and II respectively and it was also detected by PCR on BCC in 22.2% and 21.7% of group I and II respectively. EMS revealed HSV like particles in 16.6% of cases. IgG, specific gG-2 IgG and IgM were detected in 30%, 16% and 6% of cases respectively. The different assays were evaluated in relation to PCR on bladder tissue biopsies. The gG-2-based ELISA and EMS on BCC were found to be highly specific (97.3% and 100% respectively), with similar low sensitivity of approximately 54%. PCR on BCC was the most sensitive assay. The association of HSV-2 with bladder cancer is suggested especially in schistosomal patients.


Subject(s)
Cystitis/virology , Herpes Simplex/complications , Herpesvirus 2, Human/isolation & purification , Urinary Bladder Neoplasms/virology , Adolescent , Adult , Aged , Antibodies, Viral/blood , Biopsy , Cystitis/epidemiology , Cystitis/immunology , DNA, Viral/chemistry , DNA, Viral/genetics , Egypt/epidemiology , Female , Herpes Simplex/epidemiology , Herpes Simplex/immunology , Herpes Simplex/virology , Herpesvirus 2, Human/genetics , Humans , Immunoenzyme Techniques , Male , Microscopy, Electron , Middle Aged , Polymerase Chain Reaction , Seroepidemiologic Studies , Urinary Bladder Neoplasms/epidemiology , Urinary Bladder Neoplasms/immunology , Young Adult
2.
Medscape J Med ; 10(10): 232, 2008.
Article in English | MEDLINE | ID: mdl-19099026

ABSTRACT

BACKGROUND: Bladder cancer is a common malignancy in Egypt. Human papillomavirus (HPV) could have a possible etiologic role in bladder carcinogenesis. We aimed to estimate the prevalence of HPV-16, -18, and -52 in Egyptian patients with bladder cancer or recurrent cystitis, and to study the correlation of type-specific HPV-immunoglobulin (Ig)G with polymerase chain reaction (PCR) results and different clinicopathologic parameters. METHODS: This study was conducted on 60 inpatients of the Urosurgery Department at the Theodor Bilharz Research Institute (TBRI), who were identified histopathologically and clinically as cancer bladder (group I, 20 patients), cystitis (group II, 24 patients), and cancer bladder with cystitis (group III, 16 patients), and a fourth group of 20 healthy control subjects (for serologic testing). Patients were subjected to detection of HPV-16 and -18 DNA by PCR on bladder tissue biopsies (BTB) and buffy coat cells (BCC) and serum IgG antibodies to L1 capsids of HPV-16 and -52 IgG by enzyme-linked immunosorbent assay (ELISA). RESULTS: HPV-16 and -18 DNA were detected in BTB (30% and 10%, respectively) with significantly higher rates (44.4%) in bladder cancer than cystitis cases (11.11%), with significant association with schistosomal affection (78.6% and 25%, respectively) and recurrence (48%, HPV-16). There was a significant association of transitional cell carcinoma (TCC) with HPV-16 in 69.2% and 61.1% of BCC and BTB, respectively. Multiple HPV types 16, 18, and 52 were significantly higher than single types (79.2% and 20.8%, respectively). The observed absolute association between seropositivity of HPV-52 (11.7%) and HPV-16 (26.7%) was significantly associated with TCC in patient groups only. CONCLUSION: Our study confirmed the significant association of HPV-16, -18 and -52 with bladder cancer in Egyptian patients, with the suggestion of viral synergistic action in bladder carcinogenesis. Such HPV types were significantly associated with TCC tumors of low grade and high stage, with schistosomal affection and recurrence tendency. HPV serology would pave the way for better management and follow-up of patients and for optimal design and evaluation of HPV vaccination.


Subject(s)
Cystitis/virology , Human papillomavirus 16/isolation & purification , Human papillomavirus 18/isolation & purification , Urinary Bladder Neoplasms/virology , Adolescent , Adult , Aged , Egypt , Female , Humans , Male , Middle Aged , Papillomavirus Infections , Recurrence , Young Adult
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