1.
J Chromatogr B Biomed Sci Appl
; 710(1-2): 27-35, 1998 Jun 12.
Article
in English
| MEDLINE
| ID: mdl-9686868
ABSTRACT
A method was developed for the determination of the specific activities of leucine and phenylalanine in plasma using a flow-through scintillation counter coupled with high-performance liquid chromatography components. Results were compared with those obtained from liquid scintillation counting. Differences in the specific activities of leucine and phenylalanine between the two methods were not statistically significant. We concluded that flow-through radioactivity detection can be used for quantitative amino acid assays. However, the minimum activity that can be detected may be prohibitively low in certain applications.