ABSTRACT
Majority of dyes are toxic to all the living organisms and inherently resistant to microbial degradation. Hence, decolorization and degradation of textile dye methyl red were evaluated using isolated bacterial strain Pseudomonas aeruginosa (P. aeruginosa). Methyl red dye decolorization by P. aeruginosa with respect to various parameters was optimized. Data shows that maximum possible decolorization was seen at 50 ppm dye concentration, 1400 mg/l glucose concentration, 700 mg/l sodium chloride (NaCl) concentration, pH 9, temperature 38°C, 1000 mg/l urea concentration P. aeruginosa AM-1 strain. The highest percent (91.1%) of bioremediation was achieved at 40 ppm dye concentration in Allium cepa test. These findings suggest P. aeruginosa strain (AM-1) has the potential to be used in the biological treatment of highly toxic dye which is main constituent of dyeing mill effluents due to its high decolorization activity with simple conditions. Strain AW-1 strain also has potential to bioremediate other wastewater containing methyl red dye.
ABSTRACT
Increased amounts of toxicants may cause sever health issues in humans as well as in aquatic life. Scientists are developing new technologies to combat these problems. Biological methods of detoxification are always beneficial for the environment. Pseudomonas fluorescens is known for its detoxification capacity. In this study Pseudomonas fluorescens stains were isolated from different locations of the Ha'il region, Saudia Arabia. The microbial strain AM-1 displayed resistance to heavy metals (Cr6+, Ni2+, Cd2+, Pb2+) and pesticides (BHC, 2,4-D, Mancozeb) at pollutant levels typical of highly contaminated areas. Additionally, AM-1 exhibited substantial detoxification potential, reducing toxicity by 40.67% for heavy metals and 47.4% for pesticides at 3x concentrations. These findings suggest that the AM-1 strain supports environmental remediation and pollution mitigation. Atomic absorption spectrometry (AAS) results exhibited bioremediation efficiency for metals Cr6+, Ni2+, and Pb2+ using immobilized cells of P. fluorescens AM-1 isolate, estimated to be 60.57%, 68.4%, and 53.93% respectively. These findings show that AM-1 strain has a potential role in bioremediation of water pollutants and may have future implications in wastewater treatment.
