ABSTRACT
No disponible
Subject(s)
Humans , Arthritis, Rheumatoid/drug therapy , Methotrexate/pharmacology , Antirheumatic Agents/pharmacology , Methotrexate/economics , Antirheumatic Agents/economics , Drug Therapy, Combination , Treatment OutcomeABSTRACT
The cytostatic activities of Agave intermixta L. (Agavaceae) and Cissus sicyoides L. (Vitaceae) have been determined. In the antimitotic assay, Agave intermixta L. showed complete inhibition of cell division at 24 h of treatment. Both species showed a moderate cytostatic activity against HEp-2 cells, Cissus sicyoides L. being the most active species.
Subject(s)
Antineoplastic Agents/pharmacology , Mitosis/drug effects , Plants, Medicinal , Animals , Artemia/drug effects , Caribbean Region , Cell Death/drug effects , Hepatocytes/drug effects , Humans , Medicine, Traditional , Mitotic Index , Plant Extracts/pharmacology , Plant Leaves/chemistry , Plant Roots/cytologyABSTRACT
OBJECTIVE: Lactobacilli are considered cariogenic micro-organisms. As oral species of lactobacilli have not been thoroughly described, the aim of this work was to isolated and identify these organisms from teeth, tongue, saliva and gum of healthy patients and to describe some of their surface properties. SUBJECTS: Forty-four subjects from Tucumán, Argentina, with D, d and M, m indices equal to 0. MATERIALS AND METHODS: Samples were obtained from different areas of the oral cavity. Microorganisms were cultured in lactobacilli selected media (LBS) and identified morphologically and biochemically. Hydrophobicity was analysed by partition in organic solvents, acidity by affinity with chloroform and basicity with ethyl acetate (MATH method), aggregation and coaggregation in presence of (NH4)2SO4, and haemagglutination with ABO erythrocytes in microplates. RESULTS: Eighty-five lactobacilli were isolated; 29.4% were homofermenter, 44.7% facultative heterofermenter and 25.9% obligate heterofermenter. Predominant species were L. fermentum, L. plantarum, L. salivarius, and L. rhamnosus. Most of the strains showed moderate to high hydrophobicity and demonstrated high acid and basic surface charges with almost 40% showing salt aggregation. Few strains haemagglutinated. CONCLUSIONS: A variety of Lactobacillus species were isolated from healthy mouths, some of whom showed adhesion-related properties such as high hydrophobicity and charged surfaces. Probable mechanisms related to the ecological behaviour of lactobacilli in the oral cavity are discussed.
Subject(s)
Lactobacillus/classification , Mouth/microbiology , Acetates/chemistry , Adolescent , Adult , Ammonium Sulfate/chemistry , Analysis of Variance , Bacterial Adhesion , Child , Chloroform/chemistry , Culture Media , DMF Index , Dental Caries/microbiology , Ecology , Electrophysiology , Erythrocytes/microbiology , Female , Fermentation , Gingiva/microbiology , Hemagglutination , Humans , Hydrogen-Ion Concentration , Lactobacillus/cytology , Male , Oral Health , Saliva/microbiology , Solvents/chemistry , Tongue/microbiology , Tooth/microbiology , Water/chemistryABSTRACT
Oleuropein, tyrosol, squalene and the fraction of sterols and triterpenoid dialcohols from the unsaponifiable fraction obtained from virgin olive oil have been tested for possible cytostatic activity against McCoy cells, using 6-mercaptopurine as a positive control. The samples of sterols and triterpenic dialcohols showed a strong activity.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Plant Oils/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Division/drug effects , Cell Line , Humans , Iridoid Glucosides , Iridoids , Olive Oil , Phenylethyl Alcohol/analogs & derivatives , Phenylethyl Alcohol/pharmacology , Pyrans/pharmacology , Squalene/pharmacology , Sterols/pharmacology , Triterpenes/pharmacologyABSTRACT
The effects of hexanoic extracts of Viscum cruciatum Sieber parasitic on Crataegus monogyna Jacq. (I), Crataegus monogyna Jacq. parasitized with Viscum cruciatum Sieber (II), and Crataegus monogyna Jacq. non-parasitized (III), and of a triterpenes enriched fractions isolated from I, II and III (CFI, CFII, CFIII respectively), on the growth of HEp-2 cells have been evaluated. All the samples demonstrated significant cytotoxic activity against cultured HEp-2 cells, and all of them showed a stronger in vitro activity than 6-mercaptopurine solution used as a positive control. With the hexanoic extracts I, II and III almost similar activity was obtained, but the hexanoic extract I showed comparably better results. Almost complete inhibition was observed with triterpenes-enriched fractions CFI, CFII and CFIII, at the dose 6 micrograms/ml, after 72 h of treatment. The most intense response was obtained with the triterpenes-enriched fraction CFIII (from Crataegus monogyna non-parasitized), where the inhibition was 93%, but the fraction CFI and CFII showed similar inhibition (92% and 83%).
Subject(s)
Antineoplastic Agents, Phytogenic/toxicity , Cell Survival/drug effects , Mistletoe , Plants, Medicinal , Antineoplastic Agents, Phytogenic/isolation & purification , Humans , Laryngeal Neoplasms , Plant Extracts , Tumor Cells, CulturedABSTRACT
In-vivo anti-inflammatory activity of silymarin was tested in different acute inflammation experimental models. In carrageenan-induced paw oedema in rats, silymarin given orally reduced in a dose-dependent manner the food-pad abscesses (ED50 = 62.42 mg kg-1). In xylene-induced ear mouse inflammation, silymarin applied topically was more effective than administered intraperitoneally, with effects comparable with those of indomethacin. Silymarin also produced a dose-dependent inhibition of leukocyte accumulation in inflammatory exudates following intraperitoneal injection of carrageenan in mice; silymarin significantly reduced the number of neutrophils. Silymarin was unable to inhibit phospholipase A2 in an in-vitro assay. Besides its known anti-oxidative properties and its ability to act as a radical scavenger, these results suggest that silymarin exerts an important anti-inflammatory action in-vivo by reducing oedema with the effect markedly influenced by the inhibition of neutrophil migration into the inflamed site.
