[Detection and differentiation of the bovine parainfluenza-3 virus strains studied by amplification and sequencing of the HN gene]. / Vyiavlenie i differentsiatsiia shtammov virusa paragrippa-3 krupnogo rogatogo skota s pomoshch'iu polimeraznoi tsepnoi reaktsii i sekvenirovaniia fragmenta gena HN.

A possibility of using the amplification of gene HN fragment in combination with nucleotide cDNA sequencing for the purpose of identification and strain differentiation of bovine parainfluenza-3 virus was demonstrated. A comparative analysis of the primary structure in the studied HN gene fragment revealed 2 genetic groups among the investigated virus' strains and isolates. Group 1 is made up of Northern American viral strains and of Russian isolates, whose primary structure has a high level of homology to the primary SF-4/32 strain structure; group 2 comprises the virus' Russian isolates with a high level of homology to the mentioned strains to Japanese strains' sequences. The biggest differences between the studied strains and the viral isolates amounted to around 8%, when the nucleotide sequences were compared, and to around 4%, when the corresponding amino-acid sequences were compared.

[Indication, genotyping and phylogenetic analysis of bovine diarrhea virus obtained in the territory of the Russian Federation ]. / Indikatsiia virusa diarei krupnogo rogatogo skota, genotipirovanie i filogeneticheskii analiz izoliatov, vyiavlennykh na territorii Rossiiskoi Federatsii.

Bovine diarrhea virus (BDV) is widespread throughout the European part of the Russian Federation. The BDV genome is detected in around 40% of animals (aged up to 18 months) with lesions in the gastrointestinal tract and respiratory system. It can be found in all intestinal sections, including its contents and feces, in lymphoid and respiratory organs as well as in nasal washings and discharges and in vaginal tunic discharges of cows. Primary structure analysis of 5'-untransalted region of the genome of the detected isolates revealed that all of them belonged to genotype 1. Phylogenetic analysis confirmed that a part of isolates belonged to subgenotype 1a, while most isolates make up a separate group. No geographic differences were reported in the circulation of the BDV genetic variants within Russia's territory.

[Use of PCR and nucleotide sequence analysis of the VP7 gene for detection of bovine rotaviruses and identification of their G serotypes]. / Ispol'zovatie PTSR i sravnitel'nogo analiza nukleotidnoi posledovatel'nosti gena VP7 dlia vyiavlenia rotavirusov krupnogo rogatogo skota i opredeleniia ikh G-serotipov.

A Vp7 gene fragment PCR protocol was developed to detect the bovine rotaviruses and to identify their G serotypes. The most widespread bovine rotaviruses of G serotypes (G6, G8 and G10) can be distinguished on the basis of the PCR fragment size, while other G serotypes can be differentiated through a comparative analysis of the VP7 gene fragment nuclcotide sequence. Twenty-four bovine rotavirus field isolates were detected, and their G stereotypes were determined by using the method in question. Fourteen isolates were shown to be of G6 serotype; four of them were of G8, five--of G10, and one isolate was of G11 serotype. A possibility of detecting more than one isolate by this method was shown. Finally, a feasibility of using the method for searching for rotaviruses of new bovine rotavirus G serotypes and for rotaviruses, which do not belong to a so far described G serotypes, is discussed.

[Phylogenetic analysis of swine fever virus strains]. / Filogeneticheskii analiz shtammov virusa chumy plotoiadnykh.

Amplification of H-gene fragment in combination with cDNA nucleotide sequencing can be used for indication and strain differentiation of classical swine fever virus.

[Primary structure of the F-gene from Rinderpest virus strain K]. / Pervichnaia struktura F-gena virusa chumy krupnogo rogatogo skota shtamma K.

Synthesis, cDNA cloning, and nucleotide sequencing of F gene of rinderpest virus strain K was carried out. Analysis of nucleotide sequence showed the only open reading frame coding for protein from 546 a.o. with mol. weight 58.6 kDa. The mean percentage of identical nucleotide residues between F genes of strains K, Kabete O, and L is 76.4% for 5'-untranslated region and 90.5% for translated region, the share of similar amino acid residues in the respective proteins is 92.9%. The structure of restriction site of F0 precursor protein in rinderpest strains with different virulence is similar. Protein F of rinderpest virus strain K has 3 potential glycosylation sites and 13 cystein residues in positions identical to those of F protein of rinderpest strains Kabete O and L.

[Primary structure of gene H of cattle plague virus strain K]. / Izuchenie pervichnoi struktury H-gena virusa chumy krupnogo rogatogo skota shtamma K.

Synthesis, cDNA cloning, and identification of H gene nucleotide sequence of rinderpest virus (RPV) K strain are carried out. Analysis of the identified nucleotide sequence has revealed the single open reading frame encoding a protein consisting of 609 amino acids with molecular weight of 68 kDa. The mean nucleotide homology between H genes of K, Kabete O and L strains in 88.0%, the mean amino acid homology of the corresponding proteins is 88.2%. RPV K strain hemagglutinin contains 5 potential glycosylation sites. The position of all 13 cystein bases is identical to positions in H proteins of RPV Kabete O and L strains. Studies of the hydrophobic profile of the compared proteins have shown 2 potential transmembrane fragments.