ABSTRACT
Effect of atherogenous diet (cholesterol, cholic acid and vitamin D2) on the state of collagen and elastin fibrilles as well as on content of proteins in rat blood serum, myocardium and aorta was studied by means of biochemical and histochemical methods. In sclerotic impairments the following phenomena occurred: a decrease in content of blood serum albumin, soluble tissue collagen and elastin and of insoluble elastin; an increase in content of globulins and haptoglobins in blood serum as well as of insoluble collagen in aorta and myocardium. The increase in content of insoluble collagen was accompanied by stimulation of growth of rough reticular and collagen fibrilles, deposition of calcium, fragmentation of the inner elastic membrane, by medionecrosis with deterioration of elastic tissue in aorta wall, in coronary arteria and in their intramural branches. These alterations in characteristics, properties and content of blood serum and tissue fibrillar proteins reflect only a part of complex processes involved in destruction of connective tissue under conditions of sclerosis.
Subject(s)
Aorta/metabolism , Arteriosclerosis/metabolism , Diet, Atherogenic , Myocardium/metabolism , Animals , Aorta/pathology , Arteriosclerosis/pathology , Collagen/metabolism , Elastin/metabolism , Endothelium/metabolism , Rats , Rats, Inbred Strains , Serum Albumin/metabolism , Serum Globulins/metabolismABSTRACT
The effect of fundazol pesticide (methyl-l-butylcarbamoilbenzimidazol carbamate) on the development of sclerotic processes in aorta of experimental animals was followed up, induced by atherogenic diet (cholesterol, cholic acid and vitamin D2). The experiment was carried out on 144 albino rats, both sexes, administered 250 mG/kg and 1000 mg/kg fundazol orally in the course of 30 days, singly or in combination with atherogenic diet, The course of the metabolic processes was studied as well as the structural characteristic of the fibrous structures and the basic intercellular substance in the vascular wall of aorta by biochemical (enzymatic activity of some dehydrogenases and phosphatases, the quantity of soluble and insoluble collagen and elastin proteins, the content of glycosamioglycans - sulphated and unsulphated) and routine morphological and histochemical methods. The combined administration of both factors leads to potentiation of the effect of atherogenic diet by fundazol, even with a lower dose, the developing pathologic process being more severe and progressing more rapidly as compared with the single effect of the atherogenic factor.
Subject(s)
Arteriosclerosis/physiopathology , Benomyl/toxicity , Carbamates/toxicity , Herbicides/toxicity , Animals , Aorta/drug effects , Aorta/enzymology , Aorta/pathology , Diet, Atherogenic , Dose-Response Relationship, Drug , Drug Synergism , Enzyme Activation/drug effects , Female , Male , RatsABSTRACT
The effect of the pesticide Fundazol (methyl-l-butyl-carbamoil-benzimidazol carbamate) on the development of sclerotic lesions in the heart muscle of experimental animals, caused by atherogenic diet, was studied. Investigations were carried out in a series of 144 albino rats of either sex, treated accordingly with 250 mg/kg and 1000 mg/kg Fundazol or atherogenic diet alone and with a combination of atherogenic factor and either dose of the pesticide. Using biochemical test (dehydrogenase and phosphatase activities, quantity of soluble and insoluble collagen and elastin proteins, content of glucosamineglycans and glucoproteins) and routine morphologic and histochemical methods, the authors studied the metabolic processes and the characteristics of the fibrous structures and basic intercellular substance in the heart muscle. Combined with atherogenic diet, Fundazol potentiated its effect: the developing pathologic process was more severe and progressed more rapidly than in animals exposed to the atherogenic factor alone.
Subject(s)
Arteriosclerosis/chemically induced , Heart/drug effects , Herbicides/toxicity , Animals , Arteriosclerosis/pathology , Benomyl , Diet, Atherogenic , Dose-Response Relationship, Drug , Enzyme Activation/drug effects , Female , Male , Myocardium/enzymology , Myocardium/pathology , RatsABSTRACT
The morphological picture of the myocardium of white rats after administration of cholesterol, cholic acid, and vitamin D2 was studied. After four weeks of atherogenic diet the hearts of rats showed severe lesions of intramural vessels in addition to disseminated necroses, granulomatous cell reaction of the stroma which was sometimes of the giant cell nature, and connective tissue proliferation. Non-uniform hypertrophy of the endothelium, edema and defibrilation of the media, proliferation of young connective tissue cells, outgrowth of reticular and collagen fibers, and deposition of calcium salts in the entire thickness of the vessel wall were observed, as well as destruction of the inner elastic membrane and its substitution with newly formed fibers and membranes. These changes may be associated with the toxic effect and sensitizing properties of vitamin D2 which was a component of the atherogenic diet used.
Subject(s)
Coronary Disease/pathology , Myocardium/pathology , Animals , Coronary Vessels/pathology , Diet, Atherogenic , Female , Male , Necrosis , RatsABSTRACT
Qualitative and quantitative changes in hepatic tissue glycoproteins and glucosamine glycans are studied after tetrachlormethane intoxication. The experiment is conducted on 40 control and 40 laboratory male white rats, poisoned twice weekly per os with 0.1 ml CP CCI4 per 100 g of body weight, in the form of 50 per cent oil solution. Followed in dynamics (at 14, 30, 45 and 90 days), the newly formed connective tissue appears to be characterized by a reduction of glicoproteins and increase in glucosamine glycans. Qualitative changes are established in the character of polycarbohydrate components - upset stability of their complex with proteins - and in the normal correlation of the various glucosin glycans.
Subject(s)
Carbon Tetrachloride Poisoning/metabolism , Glycoproteins/metabolism , Liver/drug effects , Animals , Carbon Tetrachloride Poisoning/pathology , Connective Tissue/metabolism , Glycoproteins/analysis , Liver/metabolism , Liver/pathology , Necrosis , Rats , Time FactorsABSTRACT
The author examined the influence of abnormal amounts of iron on the development of early fibrosis (14th day), early and advanced cirrhosis (45th and 90th day) in liver esperimentaly damaged by carbon tetrachloride by means of morphological and histochemical methods. On the basis of the obtained data she could suggest that while at early stages iron did not have significant influence on the rate of development and gravity of the pathologic process, later (advanced cirrhosis) the changes were less manifested. She asumed that three valence iron, administered in the organism of the animals intoxicated by carbon tetrachloride, improved the course of the metabolic processes in the damaged liver cells, a manifestation of which was later and weaker development of the cirrhotic process.
