ABSTRACT
Postpartum reproductive disorders cause heavy economic losses in dairy sector. Uterine infections include endometritis, metritis, mucometra, and pyometra. Postpartum endometritis in dairy cows has been defined as inflammation of endometrium occurring 21 days or more after parturition without systemic signs of illness. The treatment of endometritis with antimicrobials has met with varying degrees of success, inconsistent recovery rate, high cost of treatment, milk disposal, emergence of microbial resistance, and reduced phagocytic activity of polymorphonuclear leukocytes In our country, around 20,000 medicinal plant species have been recorded, but more than 500 traditional communities use about 800 plant species for curing different diseases. Many herbs such as garlic, neem, ashwagandha, and turmeric have been tried for the treatment of endometritis in cows with a good success.
ABSTRACT
AIM: The present study was undertaken to standardize a convenient method for isolation and purification of ß-lactoglobulin (ß-lg) from cow milk keeping its antigenicity intact, so that the purified ß-lg can be used for detection of cow milk protein intolerance (CMPI). MATERIALS AND METHODS: Raw milk was collected from Gir breed of cattle reared in Haringhata Farm, West Bengal. Milk was then converted to skimmed milk by removing fat globules and casein protein was removed by acidification to pH 4.6 by adding 3 M HCl. ß-lg was isolated by gel filtration chromatography using Sephacryl S-200 from the supernatant whey protein fraction. Further, ß-lg was purified by anion-exchange chromatography in diethylaminoethyl-sepharose. Molecular weight of the purified cattle ß-lg was determined by 15 percent one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was analyzed by gel documentation system using standard molecular weight marker. RESULTS: The molecular weight of the purified cattle ß-lg was detected as 17.44 kDa. The isolated ß-lg was almost in pure form as the molecular weight of purified ß-lg monomer is 18kDa. CONCLUSION: The study revealed a simple and suitable method for isolation of ß-lg from whey protein in pure form which may be used for detection of CMPI.
ABSTRACT
A total of 113 diarrheic samples comprising of 68 buffalo calves and 45 cow calves were screened by RNA-PAGE for the detection of presence of rotavirus. RNA-PAGE analysis of these samples revealed 11 (9.73%) was found positive for rotavirus. Out of 68 faecal samples of buffalo calves tested for viral gastroenteritis, 8 (11.76%) were found positive for rotavirus. Similarly, out of 45 faecal samples of cattle calves tested for viral gastroenteritis, 3 (6.66%) was found positive for rotavirus. Rotavirus-positive samples represented long electropherotype. All RNA-PAGE-positive faecal samples for rotavirus subjected to RT-PCR for VP7 gene, ten samples yielded a specific product of 1,013 bp of VP7 gene. All the PCR-positive samples of the present study were subjected to genotyping with primers for G6, G8 and G10 genotype. All positive samples showed G10 genotype. This indicates that G10 may be predominant genotype among bovine calves in Mumbai region in India.
