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Objective: This study analyzed the current research hotspots and future development trends of the therapeutic effects of microRNA on PNI axonal regeneration through bibliometric methods. Moreover, the current advantages and disadvantages of this field as well as future development prospects are discussed in depth. Methods: CiteSpace V and VOSviewer were used as bibliometric tools to complete the analysis of the research focus and direction of the published articles. To supplement, sort out, and summarize, we analyzed the research status of the study on the application of microRNAs for axonal regeneration after peripheral nerve injury from 2013 to 2023. Results: A total of 207 publications were retrieved from the Web of Science database. After exclusion and screening, a final selection of 174 articles that met the research criteria. These 174 articles were authored by a total of 846 individuals, representing 24 countries and 199 institutions. Additionally, this study presents information on the annual publication output, country distribution, top 5 contributing authors, top 5 most cited articles, and top 10 contributing institutions. Conclusion: As one of the hottest topics today, microRNAs have become the current research hotspot in neural inflammation, neural cell repair and regeneration, neural protection, and functional recovery. With more investment in research in this field, more high-quality articles will be published in both domestic and international outstanding journals, which will bring a new era for the treatment of peripheral nerve injury.
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Objective: Pedicle screw loosening is one of the main complications after pedicle screw fixation. However, there are few reliable measures for prediction of screw loosening. The current study was carried out to find an effective method to use preoperative CT scanning as a predictor of screw loosening in the elderly patients and provide guidance for preoperative surgical planning. Methods: Patients who were treated with lumbar pedicle screw fixation procedure in our department for degenerative lumbar disorders between January 2015 and January 2021 were retrospectively included in the current study. CT scan attenuation of each vertebra was measured with Hounsfield units (HU). Screw loosening was determined in postoperatively X-ray tests. One-way analysis of variance (ANOVA) and receiver operating characteristic (ROC) curve analysis were carried out with IBMSPSS 24.00 software. Results: Screw loosening was observed in 44 of 215 patients (124 male, 91 female, average age 58.4 ± 7.6 years) during a mean follow-up time of 19.0 ± 11.2 months (range 12-32 months). No significant differences were found among the patients concerning patient gender, BMI, habit of smoking, and whether or not the patient had diabetes or suffered from spondylolisthesis (P > 0.05). The average HU value of lumbar vertebra was 122.4 ± 32.8 HU in the screw loosening group and 142.4 ± 38.2 HU in the control group, and the difference was significant (P < 0.01). ROC curve analysis revealed that the average HU value of L1-L5 has a relatively larger area under the curve (AUC) of 0.689 (95% CI: 0.605-0.773). With the sensitivity of 68% and specificity of 57%, a HU cut-off value of ≤124 HU is a plausible cut-off point to predict screw loosening. Conclusions: A prospective CT scan HU value-based prediction can be used to decide whether or not to use screw augmentation methods. A cut-off L1-L5 average HU value of 124 HU can be used as an independent risk factor for screw loosening in instrumented lumbar vertebra. More predictive indexes should be involved to achieve higher sensitivity and specificity in future clinical practice.
Subject(s)
Pedicle Screws , Spinal Fusion , Spondylolisthesis , Humans , Male , Female , Aged , Infant , Child, Preschool , Pedicle Screws/adverse effects , Retrospective Studies , Prospective Studies , Spondylolisthesis/diagnostic imaging , Spondylolisthesis/surgery , Lumbar Vertebrae/diagnostic imaging , Lumbar Vertebrae/surgery , Tomography, X-Ray Computed , Spinal Fusion/methodsABSTRACT
OBJECTIVE: To test if preoperative planning with 3 dimensional (3D)-printed spine models can increase the effectiveness and safety of spinal deformity surgery. METHODS: A total of 53 patients who were treated in our center for spinal deformities from January 2010 to January 2018 were included in the current study. They were divided into two groups based on whether 3D-printed models were used in the surgical planning. A total of 28 patients who were treated with 3D-printed models were assigned to the experimental group, and 25 patients who were treated with conventional methods were assigned to the control group. Duration of surgery, intraoperative hemorrhage, incidence of surgery related complications, Oswestry disability index (ODI), visual analogue scale (VAS), and Cobb's angle were compared between the two groups before and after surgery. RESULTS: There were significant differences in the duration of surgery, intraoperative hemorrhage and intraoperative x-ray exposure between the two groups (P<0.01). Cobb's angle was smaller in the experimental group than in the control group when measured three days and a year after surgery (P<0.01). Although there was no significant difference between the experimental and control groups (P>0.05), Oswestry disability index and VAS pain scores were lower a month and a year after the surgery than before the surgery (P<0.01). CONCLUSION: Surgical planning using 3D-printed spine models can decrease the operation time, intraoperative hemorrhage, and x-ray exposure, and help achieve satisfactory structural restoration in patients with severe spinal deformity.
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OBJECTIVE: To evaluate the susceptibility of pulmonary tuberculosis based on the single nucleotide polymorphism (SNP) of Toll like receptor 4 (TLR4) gene. METHODS: We searched PubMed, Web of science, EMBASE, and Chinese National Knowledge Infrastructure (CNKI) databases using mesh words: "tuberculosis", "pulmonary", "TLR4", "SNP", "Toll like receptor 4", "nucleotide polymorphism" for studies on the relations between TLR4 SNP polymorphism and the risk of pulmonary tuberculosis that were published before September 1st, 2021. Papers were selected according to the inclusion and exclusion criteria established in advance. The allele and genotype data of the four most widely studied SNP loci (rs4986791, rs4986790, rs11536889, rs10759932) in TLR4 gene were extracted and analyzed by Review Manager 5.3 software. RESULTS: 20 studies including a total of 24727 patients were included in the final meta-analysis. Results of the meta-analysis showed that the C allele of rs10759932 increased the risk of pulmonary tuberculosis (odds ratio - OR: 1.144; 95% confidence interval (CI) 1.043-1.254, P = 0.004). Compared with TT genotype, CC+CT genotype of rs10759932 and CT alone genotype significantly increased the risk of pulmonary tuberculosis (OR = 1.218, 95% CI 1.084-1.369, P = 0.001; OR = 1.227, 95% CI 1.085-1.387, P = 0.001). However, rs4986790, rs4986791 and rs11536889 had no significant correlation with the susceptibility of pulmonary tuberculosis (P > 0.05). CONCLUSION: G allele GG+GA genotype, and the GA genotype of rs4986790; C allele, CC+CT genotype, and the CC genotype of rs10759932 increased the risk of pulmonary tuberculosis, and may potentially be used as a marker for pulmonary tuberculosis diagnosis and monitoring.
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Objective: To find a preoperative computed tomography-based method to predict the incidence of sacral screw loosening and assist surgical planning. Methods: Surgically treated patients for degenerative lumbosacral disorders with rigid pedicle screw fixation of patients with L5-S1 vertebra in our center from January 2016 to January 2021 were retrospectively included in the current study. CT scan attenuation of the horizontal plane of the sacrum was measured with Hounsfield units (HU). Postoperative X-ray tests were used to diagnose screw loosening. The data was analyzed by independent sample t-tests, X 2 analysis, Pearson correlation analysis, and ROC curve analysis. Results: A total of 162 (114 male, 48 female, average age 63.7 ± 7.3 years) patients were included in the final analysis. Significant differences were found between the screw loosening group and nonloosening group concerning the HU value of the sacrum at the horizontal plane (P < 0.01). In ROC curve analysis, AUC was 0.674 (95% CI: 0.592-0.756). A cutoff of 200 HU provided 64.8% sensitivity and 62.4% specificity, and a cutoff of 150 HU provided 90.2% sensitivity. Conclusions: Analyzing 162 patients with at least 12 months of follow-up, we propose cutoff CT attenuation values of 200 HU and 150 HU to take moderate and radical measures of screw augmentation to prevent screw loosening in the sacral bone.
Subject(s)
Pedicle Screws , Sacrum , Aged , Female , Humans , Male , Middle Aged , Retrospective Studies , Sacrococcygeal Region , Sacrum/diagnostic imaging , Sacrum/surgery , Tomography, X-Ray ComputedABSTRACT
Acute spinal cord injury (SCI) is a devastating condition that results in tremendous physical and psychological harm and a series of socioeconomic problems. Although neurons in the spinal cord need neurotrophic factors for their survival and development to reestablish their connections with their original targets, endogenous neurotrophic factors are scarce and the sustainable delivery of exogeneous neurotrophic factors is challenging. The widely studied neurotrophic factors such as brain-derived neurotrophic factor, neurotrophin-3, nerve growth factor, ciliary neurotrophic factor, basic fibroblast growth factor, and glial cell-derived neurotrophic factor have a relatively short cycle that is not sufficient enough for functionally significant neural regeneration after SCI. In the past decades, scholars have tried a variety of cellular and viral vehicles as well as tissue engineering scaffolds to safely and sustainably deliver those necessary neurotrophic factors to the injury site, and achieved satisfactory neural repair and functional recovery on many occasions. Here, we review the neurotrophic factors that have been used in trials to treat SCI, and vehicles that were commonly used for their sustained delivery.
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Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the cell Transwell assay data in the article (featured in Figs. 4 and 7) were strikingly similar to data appearing in different form in another article by different authors. Owing to the fact that the contentious data in the above article had already appeared in different form in another article prior to its submission to Molecular Medicine Reports, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office never received any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in Molecular Medicine Reports 13: 441446, 2016; DOI: 10.3892/mmr.2015.4506].
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Borealin is a key component of chromosomal passenger complex, which is vital in cytokinesis. IQ domain-containing GTPase-activating protein 1 (IQGAP1) also participates in cytokinesis. The correlation between Borealin and IQGAP1 during cytokinesis is not yet clear. Here, we used mass spectrometry and endogenous coimmunoprecipitation experiments to investigate the interaction between IQGAP1 and Borealin. Results of the current study showed that Borealin interacted directly with IQGAP1 both in vitro and in vivo. Knockdown of IQGAP1 resulted in an abnormal location of Borealin in the midbody. Knocking down Borealin alone, IQGAP1 alone, or Borealin and IQGAP1 at the same time inhibited the completion of cytokinesis and formed multinucleated cells. Our results indicated that IQGAP1 interacts with Borealin during cytokinesis, and the correct localization of Borealin in the midbody during cytokinesis is determined by IQGAP1, and IQGAP1 may play an important role in regulating Borealin function in cytokinesis.
Subject(s)
Cell Cycle Proteins/metabolism , Cytokinesis , ras GTPase-Activating Proteins/metabolism , Cell Cycle Proteins/genetics , HeLa Cells , Humans , ras GTPase-Activating Proteins/geneticsABSTRACT
MicroRNAs (miRs) can lead to mRNA degradation or inhibit protein translation through directly binding to the 3'untranslational region (UTR) of their target mRNAs. Deregulation of miR10b has been reported to be associated with chondrosarcoma. However, the role of miR10b in chondrosarcoma cell migration and invasion, as well as the underlying mechanisms, has not been investigated. In the present study, it was demonstrated that miR10b was notably downregulated in the JJ012 and SW1353 chondrosarcoma cell lines compared with the TC28a2 normal chondrocyte line. Treatment with DNA demethylating agent 5aza2'deoxycytidine and histone deacetylase inhibitor 4phenylbutyric acid, or transfection with miR10b mimics promoted the expression of miR10b, which further suppressed the migratory and invasive capacities of JJ012 chondrosarcoma cells. Moreover, brainderived neurotrophic factor (BDNF) was identified as a novel target of miR10b, and its protein expression level was negatively regulated by miR10b in JJ012 cells. Furthermore, overexpression of BDNF reversed the inhibitory effect of miR10b upregulation on the migration and invasion of JJ012 cells. In addition, the data suggest that matrix metalloproteinase 1 (MMP1) may be involved in the miR10b/BDNFmediated chondrosarcoma cell migration and invasion in JJ012 cells. In conclusion, these findings suggest that miR10b/BDNF may serve as a potential therapeutic target for chondrosarcoma.
Subject(s)
Brain-Derived Neurotrophic Factor/metabolism , Cell Movement , Chondrosarcoma/pathology , MicroRNAs/metabolism , 3' Untranslated Regions/genetics , Brain-Derived Neurotrophic Factor/genetics , Cell Line, Tumor , Chondrosarcoma/genetics , Down-Regulation , Humans , Matrix Metalloproteinase 1/metabolism , Mutant Proteins/metabolism , Neoplasm Invasiveness , Up-Regulation , Wound HealingABSTRACT
OBJECTIVE: To determine the clinical efficacy and feasibility of multiple special formed titanium mesh cages (TMCs) to treat lumbo-sacral spinal tuberculosis via posterior approach. METHODS: From July, 2007 to June, 2013, 25 patients with lumbo-sacral spinal tuberculosis underwent one-stage posterior debridement, internal fi xation, and interbody fusion using multiple special formed titanium meshes. We compared the parameters as follow: the pre- and post-operative American Spinal Injury Association (ASIA) score, lumbo-sacral angle, the height of intervertebral space, visual analogue scale (VAS), and erythrocyte sedimentation rate (ESR), and observed operation time, intraoperative blood loss, and time of bone graft fusion. RESULTS: Operation time ranged from 90 to 180 min, (128±24) min in average. Blood loss in the operation ranged from 100 to 800 mL, (310±125) mL in average. The patients were followed up for 24 to 59 months, (43±7) months in average. One patient delayed healing of wound. ASIA score was improved in a certain degree in patients with neurological dysfunctions. The lumbo-sacral angle and the height of intervertebral space in the post-operation were significantly higher than those in the preoperation (P< 0.001). VAS was reduced obviously after 2 weeks of operation. The ESR recovered to the normal level 6 months after operation in all the patients. Solid fusion was achieved within 4 to 8 months, 6 months in average. No sinus tract, cerebrospinal meningitis, tuberculosis recurrence and titanium mesh subsidence were found. CONCLUSION: For lumbo-sacral tuberculosis, multiple special formed titanium mesh cages via posterior approach is safe and effective, which is good to the stability in spine reconstruction.
Subject(s)
Lumbosacral Region/surgery , Surgical Mesh , Titanium , Tuberculosis, Spinal/surgery , Blood Loss, Surgical , Bone Transplantation , Debridement , Humans , Orthopedic Procedures , Postoperative Period , Prostheses and Implants , Plastic Surgery Procedures , Spinal Fusion , SpineABSTRACT
Objective: To determine the clinical efficacy and feasibility of multiple special formed titanium mesh cages (TMCs) to treat lumbo-sacral spinal tuberculosis via posterior approach. Methods: From July, 2007 to June, 2013, 25 patients with lumbo-sacral spinal tuberculosis underwent one-stage posterior debridement, internal if xation, and interbody fusion using multiple special formed titanium meshes. We compared the parameters as follow: the pre- and post-operative American Spinal Injury Association (ASIA) score, lumbo-sacral angle, the height of intervertebral space, visual analogue scale (VAS), and erythrocyte sedimentation rate (ESR), and observedoperation time, intraoperative blood loss, and time of bone gratf fusion. Results: Operation time ranged from 90 to 180 min, (128±24) min in average. Blood loss in the operation ranged from 100 to 800 mL, (310±125) mL in average. hTe patients were followed up for 24 to 59 months, (43±7) months in average. One patient delayed healing of wound. ASIA score was improved in a certain degree in patients with neurological dysfunctions. hTe lumbo-sacral angle and the height of intervertebral space in the post-operation were signiifcantly higher than those in the pre-operation (P<0.001). VAS was reduced obviously atfer 2 weeks of operation. hTe ESR recovered to the normal level 6 months atfer operation in all the patients. Solid fusion was achieved within 4 to 8 months, 6 months in average. No sinus tract, cerebrospinal meningitis, tuberculosis recurrence and titanium mesh subsidence were found. Conclusion: For lumbo-sacral tuberculosis, multiple special formed titanium mesh cages via posterior approach is safe and effective, which is good to the stability in spine reconstruction.