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Biull Eksp Biol Med ; 107(3): 347-50, 1989 Mar.
Article in Russian | MEDLINE | ID: mdl-2653453

ABSTRACT

Fluorescence of intact lenses of F1 (CBA x C57BL6) mice at different stages of X-ray cataract induced by gamma irradiation (5.00 Gr) has been studied by synchronous scanning of fluorescence, the shift between emission and excitation wave lengths being 20 nm. The ratio between peek intensities of the nontryptophan and tryptophan fluorescence within the synchronous scanning spectra (K) has increased 3.5 times as much at the stage of singular dot-like opacities. K-parameter correlated with GSH level in the lenses (r = -0.9). According to the results achieved, K could be regarded as an informative indicator of the development of X-ray cataract at the stage previous to turbidity.


Subject(s)
Cataract/metabolism , Lens, Crystalline/radiation effects , Radiation Injuries, Experimental/metabolism , Animals , Cataract/etiology , Glutathione/analysis , Glutathione/metabolism , Glutathione/radiation effects , Lens, Crystalline/analysis , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Radiation Injuries, Experimental/complications , Spectrometry, Fluorescence , Whole-Body Irradiation
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