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Mol Biol (Mosk) ; 29(4): 813-6, 1995.
Article in Russian | MEDLINE | ID: mdl-7476947

ABSTRACT

Analysis of probe pH2-42 of locus D13S25 in genomic DNA in Moscow population has been made by PCR-RFLP using restriction endonuclease SspI. Localization of additional SspI-site at position 1240 (which has been revealed earlier by sequencing of pH2-42) was confirmed. This SspI restriction site was present in all representatives of the population, thus being not polymorphic. Consequently establishment of identity of additional site at position 1240 with polymorphic SspI site in D13S25 studied in previous investigations, requires additional data from another populations. Possible localization of polymorphic SspI-site in locus D13S25 is discussed.


Subject(s)
Chromosomes, Human, Pair 13 , DNA Probes , Genetic Markers , Base Sequence , Chromosome Mapping , DNA Primers , Deoxyribonucleases, Type II Site-Specific , Hepatolenticular Degeneration/diagnosis , Hepatolenticular Degeneration/genetics , Humans , Molecular Sequence Data , Moscow , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length
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