ABSTRACT
Background: Hippocampus is a neural structure in the temporal lobe that plays a crucial role in learning and memory. Cognitive impairment with learning disabilities is a common feature in hydrocephalus and is more prominent in adult-onset hydrocephalus. The aim of this study is to describe the morphological alterations in the pyramidal cells of the hippocampus of adult hydrocephalic mice. Method: Hydrocephalus was induced in adult albino mice by intra-cisternal injection of kaolin suspension (250 mg/ml in sterile water). They were sacrificed 7, 14 and 21 days post-induction. Morphological analysis was carried out on hematoxylin and eosin stained coronal sections of the hippocampus: the pyramidal neurons (normal and pyknotic) in the CA1 and CA3 subregions were counted and the pyknotic index (PI) was calculated. The somatic and dendritic features of Golgi stained pyramidal neurons were examined by light microscopy in both hydrocephalic and control mice. Result: The PI was significantly greater in the CA1 region of the hippocampus in the hydrocephalic groups compared to the age matched controls. The dendritic processes of pyramidal neurons in the CA1 region were fewer with shorter terminal branches in the hydrocephalic mice than in controls; this was pronounced at 7 days post-induction. In the CA3 region, there was no difference in dendritic arborization between hydrocephalic and control mice. Conclusion: Acute adult-onset hydrocephalus was associated with increased pyknosis and reduced dendritic arborization in hippocampal pyramidal cells in the CA1 but not CA3 region
Subject(s)
Golgi Apparatus , Hydrocephalus , Pyramidal CellsABSTRACT
Cisplatin (CIS), a known anticancer drug, has side effects initiated by oxidative damage which hinders its use.Raffia hookeri pulp extract (RHPE), reported to possess antioxidant activity should mitigate cisplatin toxicity. The presentstudy examined the potential of RHPE to reduce brain damage in rats exposed to cisplatin. Forty eight female rats (150 g -220 g) were randomized into four groups (n = 12) viz: Group 1 served as control received distilled water daily, Group 2received 100 mg/kg body weight of RHPE, Group 3 received CIS (7.5 mg/kg body weight, intraperitoneally) as single dose,Group 4 received 100 mg/kg body weight of CIS+RHPE. The RHPE was given orally via gavage for 14 days while the singledose of cisplatin was administered on the eighth day of experiment. Behavioral tests namely: transitions, rearings, groomingsand forelimb grip strength were carried out on 15th day of the experiment after which rats were euthanized followed byhistology and histomorphometry. Cisplatin significantly (p<0.05) reduced the percentage body weight changes, transitions,rearings, groomings and forelimb grip strength compared with the control group, whereas treatment with CIS+RHPEsignificantly (p<0.05) increased these parameters compared with Cisplatin treatment. Cisplatin also caused histologicalalterations of Purkinje neurons, pyramidal neurons of Cornu ammonis3, granule cells and cerebral cortex neurons. Itsignificantly (p<0.05) reduced the diameter of Purkinje (9.1±0.59 µm) compared with control (14.41±0.31 µm) andpyramidal neurons (11.32±0.05 µm) compared with control (17.03±0.54 µm). Rats in the CIS+RHPE had their histologyconsiderably improved compared with those of cisplatin. In conclusion, RHPE reversed the behavioural changes anddemonstrated neuroprotection against CIS-induced behavioural changes and microanatomical alterations of cerebellar,hippocampal and cerebral neurons.
