Biofilm growth and microbial contamination of dental unit waterlines at Kuwait University dental center.

Biofilm formation in dental unit waterlines and the resulting microbial contamination of the water in the system has become a significant problem. Contaminated water in the dental units is a major concern in dental clinics due to potential risk of causing infections particularly in elderly and immunocompromised patients. The aim of this study was at first to determine microbial contamination of the dental unit waterlines and then to study the efficacy of a comprehensive disinfection protocol on decreasing the microbial load. Water samples were collected before and after disinfection procedure from handpieces and water storage bottles from the dental units, a small 1-cm tubing was cut from each unit and subjected to microbiological culture on different growth media. Identification of the predominant species was achieved by 16S rRNA gene sequencing. Microbial growth was observed in samples collected from all dental units. Upon disinfection procedure, microbial contamination in the water samples and in the tubing surfaces was significantly reduced (P > 0.05). 16S rRNA gene sequencing revealed the presence of several species belonging to the genera Staphylococcus, Corynebacterium and Roseomonas, some of which are implicated in human infections. Aggravation of the biofilm growth on the tubing surfaces and the microbial contamination in the water can be effectively controlled by implementing appropriate and routine disinfection protocols. This may help protect the dental unit staff and the patients being exposed to the risk of infections.

Marginal Integrity of CAD/CAM Ceramic Crowns Using Two Different Finish Line Designs.

OBJECTIVE: Research on evaluation of crowns made by the latest contemporary dental computer-aided design/computer-aided manufacturing (CAD/CAM) systems for their marginal adaptation is scarce. The purpose of this in vitro study was to evaluate the marginal integrity of crowns fabricated by the latest Chairside Economical Restorations of Esthetic Ceramic (CEREC) system using 2 different finish line preparation designs: chamfer and shoulder. MATERIALS AND METHODS: Typodont teeth were divided equally into 2 groups, A and B. The teeth were prepared for full coverage crowns with a shoulder (group A) and chamfer (group B) finish line design. An experienced prosthodontist prepared all crown preparations. Evaluation of 6 sites per sample was completed by 2 calibrated, experienced prosthodontists using the modified US Public Health Services (USPHS) criteria. The descriptive statistics and Z-test were used to evaluate the results. RESULTS: A total of 180 teeth were included in the study (90 teeth in each group). Only 2 crowns in group A and 1 crown in group B were clinically unacceptable. There was no statistical significance (p = 0.282) between the 2 groups regarding finish line design. CONCLUSIONS: The CEREC system provides clinically acceptable crowns and can safely be utilized in dental treatment. Therefore, CAD/CAM restorations could be considered as a safe treatment modality by dental professionals.

Evaluation of a Technique for Reducing Chairside Occlusal Adjustment of Crowns.

PURPOSE: To assess whether the occlusion of metal-ceramic crowns, as received from the laboratory, and the time taken to adjust the occlusion of crowns not deemed acceptable, can be reduced by fabricating the crowns with controlled amounts of infra-occlusion during the laboratory phase. MATERIAL AND METHODS: An opposing set of typodonts, articulated in maximum intercuspal position served as the patient in an in vitro simulation. Seventy-five metal-ceramic crowns were fabricated for the mandibular right second molar with three different occlusal contact specifications: A, control group had occlusal contacts shared evenly by the crown and the neighboring teeth (n = 25); B, first experimental group had the occlusion relieved until 2 shimstock foils were able to be pulled from between the crown and the opposing tooth without tugging (n = 25); C, second experimental group had the occlusion relieved until 4 shimstock foils were able to be pulled from between the crown and the opposing tooth without tugging (n = 25). The occlusion of each crown, as received from the laboratory, was assessed using one of three categories (Excellent, Acceptable, and Poor). Chi-square analysis was used to test the differences in occlusal outcomes between the three study groups. For all of those rated "Acceptable," the time taken to adjust each crown to proper occlusion was recorded. One-way analysis of variance (ANOVA) and Bonferroni tests were carried out to compare the adjustment times across the three study groups. RESULTS: The 2-shim group had the best outcome, with 56% of the crowns rated as "Excellent" (p = 0.001). In addition, there were statistically significant differences in adjustment times between the control group (A) and the 2-shim (B) and the 4-shim (C) groups (p = 0.0001), but not between the 2-shim (B) and 4-shim (C) groups (p = 0.08). CONCLUSIONS: Metal-ceramic crowns fabricated with controlled interocclusal relief of 2- and 4-shims each required less time for chairside occlusal adjustment than crowns fabricated in the laboratory to conventional occlusal contact. However, the overall superior outcome, in terms of the possibility for immediate insertion as received from the laboratory as well as favorable chairside adjustment time, for the 2-shim prespacing suggests that this dimension is the preferred option over 4-shim prespacing to reduce occlusal inaccuracies of indirect restorations.

Effects of Impression Material, Impression Tray Type, and Type of Partial Edentulism on the Fit of Cobalt-Chromium Partial Denture Frameworks on Initial Clinical Insertion: A Retrospective Clinical Evaluation.

PURPOSE: To evaluate the effects of impression material, impression tray type, and type of partial edentulism (ie, Kennedy class) on the accuracy of fit of cobalt-chromium (Co-Cr) partial removable dental prostheses (PRDP) in terms of the number of fabricated frameworks required until the attainment of adequate fit. MATERIALS AND METHODS: Electronic case documentations of 120 partially edentulous patients provided with Co-Cr PRDP treatment for one or both arches were examined. Statistical analyses of data were performed using analysis of variance and Tukey honest significant difference test to compare the relationships between the different factors and the number of frameworks that needed to be fabricated for each patient (α = .05). RESULTS: Statistical analysis of data derived from 143 records (69 maxillary and 74 mandibular) revealed no significant correlation between impression material, tray type, or Kennedy class and the number of construction attempts for the pooled or individual arch data (P ≥ .05). CONCLUSION: In PRDP treatment, alginate can be chosen as a first-choice material, and metal stock trays can be a preferred option for making final impressions to fabricate Co-Cr frameworks.

Fluoride intake from fluids and urinary fluoride excretion by young children in Kuwait: a non-fluoridated community.

OBJECTIVES: To determine the pattern of fluid consumption, fluoride intake from the fluids and urinary fluoride excretion by children aged 1-9 years in Kuwait, a nonfluoridated community. METHODS: Using the cluster sampling technique, children aged 1-9 years were chosen from 2000 randomly selected households in Kuwait. Questionnaires were then administered to their mothers to determine the children's daily fluid intake. Fluoride concentrations in tap water as well as all brands of bottled water and beverages consumed by the children were measured, using the fluoride ion-specific electrode. Fluoride excretion was determined in 400 randomly selected children, based on fluoride/creatinine ratio. RESULTS: The mean daily fluid consumption by the children was high, being 1115-1545 ml. About 40% of the fluid intake was plain (tap and bottled) water and approximately 10% of the children drank bottled water exclusively. Fluoride concentration in tap water was low (0.04±SD 0.02 ppm), but was higher in bottled water (0.28±SD 0.40 ppm). Mean daily fluoride ingestion from fluids was 0.013-0.018 mg/kg body weight (bw). Even after allowing for fluoride ingestion from other sources, mean daily fluoride ingestion was still below 0.1 mg/kg bw set by the United States of America Institute of Medicine as the lowest-observed-adverse-effect level for moderate enamel fluorosis in children aged up to 8 years. Furthermore, the mean daily urinary fluoride excretion of 128-220 µg was below the provisional standard of 360-480 µg for optimal fluoride usage by children aged 3-5 years. CONCLUSION: Fluoride ingestion from fluids and urinary fluoride excretion by the children were below the recommendations for optimal fluoride usage. Thus, there is room for an upward adjustment of fluoride level in public drinking water supplies in Kuwait, as a caries preventive measure.

Influence of procedural variations during the laboratory phase of complete denture fabrication on patient satisfaction and denture quality.

OBJECTIVES: To compare subjective and objective outcomes of complete dentures fabricated with standard clinical protocols, but omitted selected steps during the laboratory phase. MATERIALS AND METHODS: Forty-three edentulous patients (mean age 58.1 years, SD 9.9, range 35-78), were consecutively recruited and randomly assigned to one of four groups according to selected variations of laboratory steps: Group 1 (n=10), omission of secondary casts obtained from impressions in border moulded custom trays; Group 2 (n=10), omission of secondary casts and face-bow articulator mounting; Group 3 (n=10), omission of face-bow mounting; Group 4 (n=13), no steps omitted (control). Clinical procedures for all groups were identical, and performed by senior dental students under supervision of prosthodontists, all of whom were blinded to the Group. At 1-, 4- and 12-weeks after delivery, patients rated their overall satisfaction, as well as a range of functional factors using visual analogue scales. An independent blinded prosthodontist similarly rated four domains of denture quality at the 1-week follow-up. RESULTS: No significant differences were noted among the groups in all aspects of patients' assessments at all the time points (P>0.1). There were no significant differences in prosthodontists' ratings of denture quality in any of the domains examined (P>0.1). CONCLUSION: Selected omissions of steps (face-bow mounting and/or secondary casts) during the laboratory phase of complete denture fabrication has only a minor role, if any, in subjective and objective outcomes, contrasting with the common belief that such omissions will adversely affect outcomes. CLINICAL SIGNIFICANCE: General practitioners provide most complete dentures. Many do not follow all the procedures they were taught at dental school. Our finding that omitting frequently advocated steps made no difference to patient satisfaction or to denture quality suggests that cost-effectiveness through simplifications be considered in practice and in education.

Marginal adaptation of Cerec 3 CAD/CAM composite crowns using two different finish line preparation designs.

PURPOSE: The purpose of this study was to compare marginal discrepancies of Cerec 3 CAD/CAM composite crowns, fabricated on human prepared teeth with two different finish line designs, chamfer and shoulder. MATERIALS AND METHODS: Sixteen human molar teeth were used to prepare full crowns. Eight teeth were prepared with a 1-mm-wide chamfer finish line and the other eight with a 1.2- to 1.5-mm circumferential shoulder. Cerec 3 crowns were fabricated from optical impressions using Paradigm MZ100 composite polymer. Marginal adaptation was evaluated in two ways: (1) using modified United States Public Health Service (USPHS) criteria to evaluate eight preselected sites on each crown margin, and (2) using scanning electron microscopy (SEM) to measure marginal gaps on all four axial walls with 15 measurements on each wall (60 measurements per crown). An evaluation of the number of acceptable crowns, determined by having all measured sites per tooth with margin gap size less than 100 microm, as a function of finish line design was also conducted. RESULTS: In both chamfer and shoulder groups, there were only two crowns (out of eight) with clinically acceptable ratings for all eight measurement sites according to USPHS criteria. Fisher's chi-square analysis showed that there was no statistically significant difference in marginal adaptability as a function of finish line design ( p>0.05). With SEM imaging, overall mean marginal gaps for the chamfer group were 65.9+/-38.7 microm (range 35.0 to 130.0 microm), and for the shoulder group were 46.0+/-9.2 microm (range 26.3 to 55.6 microm); this difference was not found to be statistically significant ( p>0.05). While crown assessment based on mean marginal discrepancy measurements indicated that both the chamfer and shoulder groups were considered clinically acceptable (<100 microm); crown acceptability based on all measurement sites being less than 100 mum indicated that in the chamfer and shoulder groups there were four and three acceptable crowns out of eight, respectively. The Fisher's chi-square test indicated no statistically significant difference between the groups ( p>0.05). An agreement rate of 81.2% was calculated between the two evaluation methods, modified USPHS criteria and SEM measurements. CONCLUSIONS: Based on mean marginal discrepancy measurements, the typical marginal assessment technique, Cerec 3 Paradigm MZ100 crown restorations appear to have acceptable marginal adaptability (mean discrepancies <100 microm). Thus, the evidence from this investigation would suggest that the finish line preparation design had no effect on marginal adaptation for Cerec 3 composite crowns.