Development and evaluation of a technique for spinal anaesthesia in broiler chickens.

AIMS: To develop a technique for the injection of local anaesthetic into the spinal canal of broiler chickens by first determining the ideal location for needle placement based on anatomy and histology, and then using the developed technique to assess the onset and duration of action of three doses of lidocaine. METHODS: Two-month-old Ross broiler chickens (n = 30) were used in this study. Computed tomography imaging followed by anatomical examination of fresh cadavers (n = 6) were used to identify a suitable intervertebral space for injection of local anaesthetic, and landmarks to locate this space. Histological evaluation of the microanatomy of the caudal vertebral column in another six birds was used to examine the position of the spinal cord within the canal. Spinal anaesthesia was attempted using injection of lidocaine at 0.5 mg/kg (n = 6), 1 mg/kg (n = 6), and 2 mg/kg (n = 6) via the selected intervertebral space. Analgesia was tested by pinching the skin of the pericloacal area with thumb forceps to determine the onset and duration of analgesia. Respiratory rate, and cloacal temperature were measured at 0 minutes and every 10 minutes after injection until sensation returned. RESULTS: The space between synsacrum and first free coccygeal vertebra (synsacrococcygeal space) was selected as the most suitable site for spinal injection. In this region, the dura mater adhered to the internal wall of the spinal canal, and the subarachnoid space was large indicating that injection would be into the subarachnoid rather than the epidural space. The interval to onset of analgesia was similar for all doses of lidocaine (1.5 (SD 0.7), 2 (SD 1) and 1.3 (SD 0.5) minutes for 0.5, 1 and 2 mg/kg, respectively; p = 0.604). Duration of analgesia was longer following injection with 2 than 0.5 or 1 mg/kg lidocaine (21.3 (SD 2.5) vs. 4.5 (SD 3.5) vs. 11.3 (SD 2) minutes, respectively; p = 0.002). Mean cloacal temperature decreased between 0 and 20 minutes after injection with all doses of lidocaine (p = 0.021). CONCLUSIONS AND CLINICAL RELEVANCE: Spinal anaesthesia in chickens is feasible and is a practical, inexpensive and simple technique for regional anaesthesia of the pericloacal area.

The morphological characters of the male external genitalia of the European hedgehog (Erinaceus Europaeus).

This study was conducted to depict anatomical characteristics of the penis of he-dgehog. Seven sexually mature male European hedgehogs were used. Following anaesthesia, the animals were scarified with chloroform inhalation. Gross penile characteristics such as length and diameter were thoroughly explored and measu-red using digital callipers. Tissue samples stained with haematoxylin and eosin and Masson's trichrome for microscopic analysis. The penis of the European hedgehog was composed of a pair of corpus cavernosum penis and the glans penis without corpus spongiosum penis. The urethra at the end of penis, protruded as urethral process, on both sides of which two black nail-like structures, could be observed. The lower part was rounded forming a blind sac (sacculus urethralis) with a me-dian split below the urethra. Microscopically, the penile bulb lacked the corpus spongiosum penis, but, corpus spongiosum glans was seen at the beginning of the free part. In the European hedgehog, entirely stratified squamous epithelium of penile urethra, absence of corpus spongiosum penis around the urethra and bilateral urethral glands are basically different compared with other mammals. This information is expected to contribute to comparative penile morphology as well as for testing phylogenic hypotheses and expanding knowledge about reproductive biology in this animal. (Folia Morphol 2018; 77, 2: 293-300).

The gross anatomy of the male reproductive system of the European hedgehog (Erinaceus Europaeus).

Hedgehogs are small spiny-coated insectivores. Due to their low body weight, calm character, and easy maintenance, they are kept as pets. It is therefore worthwhile to care about hedgehogs' health problems and to provide pet owners with information about their reproduction. Moreover, it is necessary to be familiar with their anatomy so as to satisfy the need to improve nutrition and medical care, even surgery. This study was carried out on five adult male European hedgehogs euthanased in a chloroform chamber. The European hedgehog's oval testes are invisible in inguinal region because they have no true scrotal sac. The testes are located in the craniocaudal direction with dorsolateral epididymal attachments. The vesicular glands, the European hedgehog's largest accessory sex glands, are lobulated structures containing dorsomedial and ventrolateral parts on each side. The prostate is an oval gland with right and left lobes. The paired bulbourethral glands are laid on the ischiocavernosus muscle. Histologically the vesicular, prostate gland ducts and ductus deferens as well as urethra separately were discharged in a diverticlum at the level of the pelvic urethra end. A sigmoid flexure exists in the proximal part of shaft body of the penis. There are two retractor penile muscles. In dorsal end of the penile glans, there is a small urethral process with two nail- -like, needle-shaped structures. They are on both sides of the urethral process. Furthermore, there are two intromittent sacs (Sacculus urethralis) in the ventral part of the end of the penis. (Folia Morphol 2018; 77, 1: 36-43).

The Branching Pattern of the Brachiocephalic Trunk in the Donkey (Equus asinus).

Donkeys are a member of the horse family (Equidae) and share a common ancestor. However, in morphological terms, donkeys and horses are regarded as two separate subgenus. Given variations in the branching pattern of the brachiocephalic trunk (BCT) in different species of animals and the use of donkeys in anatomy courses at colleges of veterinary medicine, we conducted this study in order to investigate the branching patterns of BCT and to describe detailed morphological information regarding donkeys. Seventeen mature donkeys were examined following euthanasia by exsanguination from the common carotid artery under general anaesthesia. Thirteen donkeys were embalmed and injected with coloured latex from BCT origin for better visualization of vessels. Four other donkeys were freshly studied without the embalming procedure. In all cases, the BCT was the only branch of the aortic arch and branched into the left subclavian (LSb) artery, the right costocervical trunk, the right subclavian (RSb) artery and the bicarotid trunk. The main branching pattern of the RSb was the costocervical trunk, the vertebral artery, the internal thoracic artery and the superficial cervical artery. The deep cervical artery branched from the costocervical trunk. The major branching pattern of the LSb was the vertebral artery, the internal thoracic artery and the superficial cervical artery. In donkeys, the external thoracic artery branches from the internal thoracic artery. Results obtained from this study indicate that BCT branching in donkeys has some similarities and differences as compared to that in horses and in the Caspian miniature horse.

The topography and gross anatomy of the abdominal gastrointestinal tract of the Persian squirrel (Sciurus anomalus) / La topografía y anatomía macroscópica del tracto gastrointestinal abdominal de la ardilla persa (Sciurus anomalus)

The persian squirrel (Sciurus anomalus) is the only member of the Sciuridae family found in the Middle East. It is herbivorous, feeding mostly on pine acorns and other seeds and fruits. It is a wild animal nesting in forest trees, although it is frequently found close to city gardens and parks. As Persian squirrels are also found in homes as "companion animals", veterinarian assistance may be sometimes required; this is a good reason to gain more specific knowledge of the anatomical features of this animal. Due to the scantiness of relevant literature, we carried out this study with the aim to provide further information on the topography and gross anatomy of its abdominal gastrointestinal tract (GIT). Seven animals of this species were utilized to measure the length, content weight and area surface of the relative segments of the abdominal GIT. The stomach is unilocular, lined with glandular epithelium; the small intestine is divided into duodenum, jejunum and ileum. The cecum is situated on the right side of the abdominal cavity and lacks the vermiform appendix observed in some rodents. The ascending colon is extensive, consisting of two loops and two straight parts forming a unique topographic arrangement, closely resembling the ascending colon of the horse. The transverse colon connects the ascending and the descending colon located on the left side of the abdominal cavity, and shows a distinct sigmoid flexure before entering the pelvic cavity. Overall, the in situ examination and the relative measurements of the various parts of the abdominal GIT suggest that the ascending colon is the main fermentation chamber in the Persian Squirrel.

La ardilla es el único miembro de la familia de los Sciuridae que se encuentra en el Medio Oriente. Es un herbívoro, come semillas, principalmente de pino, y fruta. Vive mayormente en el bosque en estado salvaje, aunque a veces se puede encontrar en las ciudades, en parques y jardines. Las ardillas persianas tambien se tienen en casas como mascotas y algunas veces es necesaria la atención del veterinario. Esta es una buena razón para conocer más profundamente sus características anatómicas. Teniendo en cuenta la escasa bibliografía existente a cerca de este animal, el objetivo de este estudio fue aportar nueva información sobre la anatomía topográfica y mascroscópica del tracto abdominal gastrointestinal (GIT) de la ardilla. Siete animales de esta especie han sido utilizados para medir el largo, el peso y el área de la superficie de los segmentos del GIT. El estómago es unilocular, alineado con epitelio glandular; el pequeño intestino está dividido en duodeno, jejuno e ileo. El ciego está localizado en la parte derecha de la cavidad abdominal y le falta el apéndice vermiforme que poseen los roedores. El colon ascendente es extenso, constituido de dos partes curvas y dos rectilíneas que forman una única dispoción topográfica cerrada como el colon ascendente en el caballo. El colon trasverso, que conecta el ascendente y el descendente, está localizado en el lado izquierdo de la cavidad abdominal y muestra una clara flexión sigmoide antes de entrar en la cavidad pélvica. En resumen, el examen in situ y las relativas medidas de las varias partes del GIT sugieren que el colon ascendente es la principal "cámara" de fermentación en la ardilla persiana.

Comparison of the RTS and ISS scores on prediction of survival chances in multiple trauma patients.

PURPOSE OF THE STUDY: Trauma represents the third cause of death after cardio vascular disease and tumors. Also in Iran, road accidents are one of the leading causes of death. Rapid evaluation of trauma severity and prediction of prognosis and mortality rate and probability of survival and rapid treatment of patients is necessary. One of the useful instruments for this is ISS and RTS scoring systems. This study evaluated 70 multi trauma patients in Fatemi trauma center affiliated to Ardabil University of medical science. MATERIALS AND METHODS: This study was prospective study populations were 70 trauma patients admitted in Fatemi trauma center. During the II month, and patients data was collected by clinical evaluating of patients and follow up them and arranged as a questionnaire then related findings were evaluated by SPSS software. RESULTS: The average age of patients was 37.6±23.5 years and minimum and maximum age was 1 and 85 years. The most common involved group was 10-19 years (13 men and 1 woman). 81.4% of patients (57 cases were male) and 18.6% were female (13 cases). The most common causes of trauma was car accident with 64.2% frequency (43 cases) and then motorcycle accident with 16.4% frequency (11 cases) and all injured patient due to motorcycle accident compose the age group less than 40 years old. Also car accident had the highest frequency in both gender. Other causes of trauma were fall down with 13.5% frequency (9 cases) and under debris 5.9% (4 cases). Also from 70 studied patients, 67 cases (95.7%) had blunt trauma and 3 cases (4.3%) had penetrating trauma. The most penetrating trauma occurs in ages less than 50 years and was in the range of 30-50 years. The average RTS and ISS was 10.67±1.45 and 18.11±8.64, high and low scores of ISS existed in all age groups but a low score of RTS was highest in the children age group. The average length of ICU stay was 12.14±11.11 days. Overall mortality was 15.7 (11 cases). In this study, by the ISS increasing, the mortality rate also increased. But there is no relation between the mortality rate and RTS ratio. CONCLUSION: The ISS scoring system performed better than the RTS in predicting of mortality and probability of survival and the length of ICU stay and had high accuracy and can predict patients' outcome better by ISS measuring.

Effect of Azotobacter chroococcum application on quantity and quality forage of rapeseed cultivars.

The study was conducted to determine the effect of Azotobacter chroococcum (Azotobacter) application on quality and quantity forage of the rapeseed cultivars (RGS 003, Hyolla 401 and Hyolla 330) and the possibility of cultivation the rapeseed forage in summer. The experimental design was spilt plot laid out in randomized complete block with three replications. Different levels of Azotobacter (zero and one kg hal) and cultivars were randomized to main plot and sub plot units, respectively. Results showed that Azotobacter significantly affected on all quantity traits except plant height. There were significant differences among cultivars for dry matter, biomass, sub branch number, plant height and forage glucosinolate concentration. Therefore Azotobacter can be considered as growth promoting for rapeseed in the future studies. Also, RGS003 cultivar was better than other cultivars when Azotobacter was applied.