Condition of Adrenergic Innervation Apparatus of the Thyroid Gland, Blood and Lymph Vessels, and Lymph Nodes during Correction of Hypothyrosis.

We used specific histochemical fluorescence-microscopic method of visualization of catecholamines to study adrenergic innervation of the thyroid gland tissue, blood vessels of the thyroid gland, cervical lymphatic vessel and lymph nodes in rats during correction of hypothyroidism with a bioactive formulation (Vozrozhdenie Plus balm with Potentilla alba L.). In experimental hypothyroidism, adrenergic innervation of the thyroid gland and the wall of the cervical lymph node, concentrated mainly along the arterial vessels and the cervical lymphatic vessel, retained its structural formations (plexuses and varicosities), but diffusion of catecholamines outside these formations was observed. Correction with the bioactive formulation restored of the contours of the nerve plexuses and varicosities and their brighter fluorescence in the thyroid gland and cervical lymphatic vessel and node. During correction of hypothyroidism with the bioactive formulation, reorganization of regional lymphatic vessels and nodes was more pronounced than reorganization of the thyroid gland.

The Specifics of Adrenergic Innervation of Lymph Nodes from Different Body Regions in Young, Mature, and Old Animals.

Adrenergic innervation in the tissue of the cervical, mesenteric, and popliteal lymph nodes in rats of different age groups (from 2-45 days to 24 months) was studied using the specific fluorescence-microscopic imaging of catecholamines. Adrenergic innervation in the lymph nodes of rats is present from the first days of life, but it develops and complicates during postnatal ontogeny and is fully formed by thedays 20-30 of life. By the age of one month, adrenergic innervation represents a well-formed apparatus in all parts of the node, i.e. in the capsule, trabeculae, and cortical and medullary substance. These are brightly fluorescent nerve fibers that form plexuses with regularly located multiple varicosities. In senile animals, the density and number of plexuses in all parts of the lymph node are preserved, but the brightness of plexuses decreases, plexus fibers become discontinuous, varicosities become less frequent or disappear. These changes are seen in lymph nodes from different body regions.

Adrenergic Innervation of the thyroid Gland, Blood and Lymph Vessels, and Lymph Nodes in Hypothyroidism.

Adrenergic innervation in the tissue of the thyroid gland, blood vessels of the thyroid gland, cervical lymphatic vessel, and lymph nodes in rats with hypothyroidism was studied by using a specific histochemical fluorescent-microscopic method of visualization of catecholamines. The presence of adrenergic innervation in the blood and lymph vessels and nodes was demonstrated. In hypothyroidism, diffusion of norepinephrine from nerve fibers and varicose thickenings was observed in the wall of the upper and lower thyroid arteries and adjacent cervical lymphatic vessels and nodes.

[Catecholamine content in the wall of the thoracic duct of the dog during postnatal development]. / Soderzhanie katekholaminov v stenke grudnogo limfaticheskogo protoka sobaki v protsesse postnatal'nogo razvitiia.

Studies have been made on the content of catecholamines (norepinephrine, adrenaline, dihydroxyphenylalanine) in the wall of the thoracic lymphatic duct of puppies during their first 3 months of postnatal life. It was found that within this period, the content of norepinephrine increases, that of adrenaline remains unchanged, whereas dihydroxyphenylalanine is detected only in animals of the first month. The increase in norepinephrine content indicates gradual maturation of the adrenergic innervation in the wall of the lymphatic duct.

[Development of sympathetic influences on lymph flow in the postnatal ontogeny of dogs]. / Razvitiie simpatcheskikh vliianii na limfotok v postnatal'nom ontogeneze u sobak.

Studies have been made on functional development of influences of the splanchnic nerve upon the lymph flow in puppies. It was shown that during the first week of postnatal life of puppies, the thoracic duct functionally is not yet ready to participate in vasomotor reactions of the organism to stimulation of the splanchnic nerve. Active role of the thoracic duct in vasomotor reactions to stimulation of the splanchnic nerve was initially noted after the first week of postnatal life of animals.

[Structure of the wall of thoracic lymphatic duct of dogs in postnatal ontogeny]. / Izuchenie stroeniia stenki grudnogo limfaticheskogo protoka u sobak v postnatal'nom ontogeneze.

Light and electronmicroscopic studies have been made on the structure of the wall of the thoracic lymph duct in puppies within 3 months of their postnatal life. It was shown that differentiation and specialization of the smooth muscle cells is accomplished in the main features to the 3rd month of postnatal life. Within this period, the number of the nervous fibers increases, and their linking to the media becomes more close. Absence of dense neuro-muscular contacts indicates distant pattern of the effect of neuromediators on the effector cells. The data obtained demonstrate that during postnatal ontogenesis of dogs changes take place in the barrier properties of the wall of the lymph duct, as well as the formation of morphological substrate for active contractile activity.

[The adrenergic innervation of the thoracic lymphatic duct in dogs in postnatal ontogeny]. / Adrenergicheskaia innervatsiia grudnogo limfaticheskogo protoka u sobak v postnatal'nom ontogeneze.

Using histochemical fluorescence technique, studies have been made on the adrenergic innervation of the wall of the thoracal lymphatic duct in postnatal development of puppies. Adrenergic fibers were found in the adventitial layer of the duct. During postnatal development of the animals, adrenergic innervation of this duct becomes more perfect, reaching the level of adult animals to the age of 2-3 months.