Sexual Norms Across Pornography Use, Sexual Fantasy, and In-Person Sexuality.

Sexual norms define perceptions of who is acceptable to partner with, how many partners are appropriate, and what sexual behaviors are acceptable to engage in. This shapes, in part, who has access to sexual pleasure and who is minoritized based on their sexuality. Though well theorized and researched for "in-person" sexuality, much less is known about sexual norms in other contexts/modalities, such as porn use and sexual fantasy, or how norms connect across these contexts. In the present study, we investigated sexual norms in porn, fantasy, and in-person sexuality, and similarities or differences between these. In an online study, gender/sex and sexually diverse participants (N = 706) manipulated digital circles representing porn use, sexual fantasy, and in-person sexuality. They used circle overlap to represent branchedness (i.e., distinction) and coincidence (i.e., similarity) in norm content, and circle size to indicate perceived norm strength. We found evidence that norm content was perceived to be more branched (i.e., distinct) than coincident (similar) and that norm strength for each context was high. This provides evidence that when people engage in each of these sexual contexts, they tend to do so through distinct normative lenses, rather than a singular lens that represents a universal set of norms applying across all sexual situations. This has implications for how we understand the associations between porn use, fantasy, and in-person sexuality, and highlights the importance of attending to sexuality in context.

Sexual fantasy research: A contemporary review.

Understanding sexual fantasies is central to understanding human sexuality. The current review synthesizes recent trends and findings in sexual fantasy research and points to several important conclusions. First, few sexual fantasies appear to be statistically unusual or rare. Second, while the bulk of sexual fantasy research to date has focused on young, cisgender, heterosexual adults in North America, studies that have accounted for diversity (e.g., LGBTQ+ inclusion, cross-cultural work) reveal multiple similarities in sexual fantasy content, but also several notable differences. Third, what people fantasize about is not necessarily synonymous with what they are interested in or do in person. Limitations and directions for future research on sexual fantasy are discussed.

Gender Inequities in Household Labor Predict Lower Sexual Desire in Women Partnered with Men.

Low sexual desire in women is usually studied as a problem, one that is located within women. However, other possibilities exist, including known gender inequities related to heteronormative gender roles. In this study, we provide the first test of the theory that heteronormativity is related to low sexual desire in women partnered with men, focusing specifically on inequities in the division of household labor. In two studies with women who were partnered with men and had children (Study 1, N = 677; Study 2, N = 396), performing a large proportion of household labor was associated with significantly lower sexual desire for a partner. Together, the results suggest that this association was mediated by both perceiving the partner as a dependent and perceiving the division of labor as unfair. These results support the heteronormativity theory of low sexual desire in women partnered with men, and show that gender inequities are important, though understudied, contributors to low desire in women partnered with men.

Sexual Orientation Across Porn Use, Sexual Fantasy, and In-Person Sexuality: Visualizing Branchedness and Coincidence via Sexual Configurations Theory.

Sexual orientation describes sexual interests, approaches, arousals, and attractions. People experience these interests and attractions in a number of contexts, including in-person sexuality, fantasy, and porn use, among others. The extent to which sexual orientation is divergent (branched) and/or overlapping (coincident) across these, however, is unclear. In the present study, a gender/sex and sexually diverse sample (N = 30; 15 gender/sex/ual minorities and 15 majorities) manipulated digital circles representing porn use, in-person sexuality, and fantasy on a tablet during in-person interviews. Participants used circle overlap to represent the degree of shared sexual interests across contexts and circle size to indicate the strength and/or number of sexual interests within contexts. Across multiple dimensions of sexual orientation (gender/sex, partner number, and action/behavior), we found evidence that sexual interests were both branched and coincident. These findings contribute to new understandings about the multifaceted nature of sexual orientations across contexts and provide a novel way to measure, conceptualize, and understand sexual orientation in context.

Highly enantioselective conjugate addition of thiols using mild scandium triflate catalysis.

A Sc complex of (4S,5S)-diphenyl PYBOX 1 was found to serve as a catalyst for the asymmetric conjugate addition reactions between various thiols and 3-crotonoyl-2-oxazolidinone, affording the corresponding adducts in good yields and high enantioselectivies (up to 92% ee). A new improved method for making (4S,5S)-diphenyl PYBOX is presented.

Detecting local establishment strategies of wild cherry (Prunus avium L.).

BACKGROUND: P. avium, a pioneer tree species that colonizes early forest successional stages, is assumed to require an effective strategy allowing stably repeatable rounds of local establishment, dispersal and local extinction. Consequently, the early replacement of cherry by climax tree species makes the establishment of several local generations very unlikely, especially in central European continuous cover forests. This has to be seen in connection with the mixed reproduction system involving asexual reproduction as a complementary adaptational strategy. Tests of the local establishment of wild cherry must therefore consider the possibility of first generation establishment via seedling recruitment potentially followed by an asexual generation (root suckering). Successful establishment can therefore be determined only among adult individuals with the option of detecting vegetative reproduction at these stages. To test the implied suggestion about local establishment strategies of wild cherry, nuclear microsatellites were used to analyse patterns of asexual propagation among adult stages that have been subjected to one of two major types of forest management. These management types, the historical "coppice with standards system" (CWS) and the "high forest system" (HFS), can be reasonably assumed to have affected the reproduction system of P. avium. RESULTS: Clear differences were found in the reproduction pattern between two stands representing the two forest management types: 1) Clonal propagation is observed in both management systems, but with a distinctly higher frequency in the CWS. Hence, sexual recruitment as a first local generation is followed by a second asexual generation in both, whereas in the CWS there is evidence for an additional clonal generation. 2) The estimation of amounts of clonal reproduction critically depends on the assumptions about multilocus gene associations. This is revealed by the application of newly developed methods of quantifying gene associations. 3) Haplotype diversities are higher in the CWS and found to be associated with a large degree of heterozygosity for the second largest clonal group. 4) Seed set was sparse over the last eight years of observation in the CWS stand. CONCLUSION: This study provides useful guidelines for more comprehensive investigations, particularly on the interrelationships between degrees of cloning and capacity of sexual reproduction, amounts of multilocus gene associations, effects of heterozygosity on cloning success, and sustainability of different forest management types.

Novel crown ether and salen metal chelation driven molecular pincers.

The synthesis of novel metal chelation driven molecular pincers based on crown ether and salen ligand substructures are described. Their functionality was monitored by fluorescence spectroscopy using pyrene groups as fluorescence probes. The pincer was shown to function reversibly with respect to metal chelation with Zn2+ and also to chelate ditopically with 100 mol % of Zn2+ and 100 mol % Li+ or 200 mol % of Li+ ion.

Primary intrapelvic seminoma in Klinefelter's syndrome.

Seminoma arising in patients with Klinefelter's syndrome is extremely rare; to our knowledge, only three cases have been reported in the English language literature. We report a case of intrapelvic seminoma in a 39-year-old man with Klinefelter's syndrome. Gross examination revealed that the tumor was a solid and irregular mass measuring 90 mm in diameter. The cut surfaces of this ill-defined tumor were yellow-white with necrotic foci. Histologically, the tumor cells were separated into lobules by branching, fibrous septa containing lymphocytes. In some parts of the tumor, a cord-like arrangement of tumor cells was present. Immunohistochemically, the tumor cells were strongly and diffusely positive for antiplacental alkaline phosphatase antibody along their cytoplasmic membranes, but negative for both chorionic gonadotrophin and alpha-fetoprotein. Based on these findings, we diagnosed this tumor as a seminoma. The testes when examined were found to be atrophic bilaterally, but with no tumor lesions. Chromosomal analysis yielded a 47XXY karyotype, compatible with Klinefelter's syndrome. These findings indicate a case of primary intrapelvic seminoma in Klinefelter's syndrome. The patient underwent intensive radiation therapy postoperatively, and he demonstrated no evidence of recurrence or metastasis during the 13-month period following surgery.

Protein conformation change of myoglobin upon ligand binding probed by ultraviolet resonance Raman spectroscopy.

Conformational change of myoglobin (Mb) accompanied by binding of a ligand was investigated with 244 nm excited ultraviolet resonance Raman Spectroscopy (UVRR). The UVRR spectra of native sperm whale (sw) and horse (h) Mbs and W7F and W14F swMb mutants for the deoxy and CO-bound states enabled us to reveal the UVRR spectra of Trp7, Trp14, and Tyr151 residues, separately. The difference spectra between the deoxy and CO-bound states reflected the environmental or structural changes of Trp and Tyr residues upon CO binding. The W3 band of Trp7 near the N-terminus exhibited a change upon CO binding, while Trp14 did not. Tyr151 in the C-terminus also exhibited a definite change upon CO binding, but Tyr103 and Tyr146 did not. The spectral change of Tyr residues was characterized through solvent effects of a model compound. The corresponding spectral differences between CO- and n-butyl isocyanide-bound forms were much smaller than those between the deoxy and CO-bound forms, suggesting that the conformation change in the C- and N-terminal regions is induced by the proximal side of the heme through the movement of iron. Although the swinging up of His64 upon binding of a bulky ligand is noted by X-ray crystallographic analysis, UVRR spectra of His for the n-butyl isocyanide-bound form did not detect the exposure of His64 to solvent.

[Branched alkanes and other apolar compounds produced by the cyanobacterium Microcoleus vaginatus from the Negev desert]. / Razvetvlennye alkany i drugie nepoliarnye soedineniia, produtsiruemye tsianobakteriei Microcoleus vaginatus iz pustyni Negev.

Gas chromatography-mass spectrometry on serially coupled capillary columns with different polarity of stationary phases showed that the soil cyanobacterium Microcoleus vaginatus from the Negev desert produces an unusual mixture of 4 normal and more than 60 branched alkanes, as well as a number of fatty acids, cyclic and unsaturated hydrocarbons, aldehydes, alcohols, and ketones. The dominant compounds were heptadecane (12%), 7-methylheptadecane (7.8%), hexadecanoic acid (6.5%), (Z)-9-hexadecenoic acid (5.6%), 4-ethyl-2,2,6,6-tetramethylheptane (2.8%), (Z)-9-octadecenoic acid (2.8%), and 4-methyl-5-propylnonane (2.7%).

Heme structure of hemoglobin M Iwate [alpha 87(F8)His-->Tyr]: a UV and visible resonance Raman study.

Heme structures of a natural mutant hemoglobin (Hb), Hb M Iwate [alpha87(F8)His-->Tyr], and protonation of its F8-Tyr were examined with the 244-nm excited UV resonance Raman (UVRR) and the 406.7- and 441.6-nm excited visible resonance Raman (RR) spectroscopy. It was clarified from the UVRR bands at 1605 and 1166 cm(-)(1) characteristic of tyrosinate that the tyrosine (F8) of the abnormal subunit in Hb M Iwate adopts a deprotonated form. UV Raman bands of other Tyr residues indicated that the protein takes the T-quaternary structure even in the met form. Although both hemes of alpha and beta subunits in metHb A take a six-coordinate (6c) high-spin structure, the 406.7-nm excited RR spectrum of metHb M Iwate indicated that the abnormal alpha subunit adopts a 5c high-spin structure. The present results and our previous observation of the nu(Fe)(-)(O(tyrosine)) Raman band [Nagai et al. (1989) Biochemistry 28, 2418-2422] have proved that F8-tyrosinate is covalently bound to Fe(III) heme in the alpha subunit of Hb M Iwate. As a result, peripheral groups of porphyrin ring, especially the vinyl and the propionate side chains, were so strongly influenced that the RR spectrum in the low-frequency region excited at 406.7 nm is distinctly changed from the normal pattern. When Hb M Iwate was fully reduced, the characteristic UVRR bands of tyrosinate disappeared and the Raman bands of tyrosine at 1620 (Y8a), 1207 (Y7a), and 1177 cm(-)(1) (Y9a) increased in intensity. Coordination of distal His(E7) to the Fe(II) heme in the reduced alpha subunit of Hb M Iwate was proved by the observation of the nu(Fe)(-)(His) RR band in the 441.6-nm excited RR spectrum at the same frequency as that of its isolated alpha chain. The effects of the distal-His coordination on the heme appeared as a distortion of the peripheral groups of heme. A possible mechanism for the formation of a Fe(III)-tyrosinate bond in Hb M Iwate is discussed.

[Primary treatment for stage D2 prostate cancer: a randomized study of combined androgen blockade alone versus combined with UFT].

To evaluate the efficacy of chemoendocrine therapy for the initial treatment of stage D2 prostate cancer, we conducted a prospective randomized study which compared combined androgen blockade alone to that combined with UFT. Twenty-one patients received LH-RH agonist and flutamide (Group-A), and 23 patients received LH-RH agonist, flutamide and UFT (Group-B). The overall response rate and the PSA response rate of Group-A was 71.4% and 100% respectively, against 65.2% and 90%, respectively in Group-B. The median follow-up period was 24 months. The 2-year progression-free survival rate of Group-A was 7.4% and that of Group-B was 15.9%. The response rate and progression-free survival rate did not differ significantly between the 2 groups. Liver dysfunction due to flutamide was common in both groups, and a total of 4 patients did not continue the treatment because of this adverse effect. We conclude that in patients with stage D2 prostate cancer, treatment with combined androgen blockade and UFT is not superior to treatment with combined androgen blockade alone.

Expression of CD44 splice variants in bladder cancer.

BACKGROUND: Recently, it was noted that CD44 participates in a wide variety of cell-cell or cell-matrix interactions including tumor metastasis and the altered expression of CD44 splice variants is helpful in the diagnosis of colon and breast cancer. The purpose of the study was to investigate altered expression of CD44 in bladder cancer. METHODS: Thirty-three surgical specimens of 22 patients and seven bladder cancer cell-lines were analyzed by various methods including RT-PCR-Southern blotting. Northern blotting, and flow-cytometric analysis. RESULTS: In RT-PCR analysis, the hematopoietic variant and numerous additional variants were detected in all cell-lines and almost all surgical specimens. In three out of seven cell-lines of bladder cancer (42.9%), especially numerous larger bands (ladder pattern) were detected using RT-PCR. These three cell-lines expressed a smaller quantity of CD44 mRNAs and CD44 molecules than the other four cell-lines. In eighteen out of 22 (81.8%) bladder cancer specimens, we detected larger variants that exceed 1500 base pairs, compared with the detection in four out of eleven (36.4%) visually normal mucosa specimens (P < 0.05). However, there was no correlation between the expression of the larger variants and clinicopathological features. CONCLUSION: The quantitative and qualitative change of CD44 variants were demonstrated in bladder cancer.

High-level expression of the CD44 variant sharing exon v10 in renal cancer.

To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription-polymerase chain reaction (RT-PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, we utilized the RT-PCR Southern blot method. One out of 19 (5.3%) renal cancer specimens expressed a larger molecular weight band than 1 kb by RT-PCR analysis, in contrast to previous findings in colon and breast cancer. The band patterns in RT-PCR were different in 14/17 (82.4%) cases between normal kidney and tumors, and a band of about 700 bp was especially marked in 12/17 (70.6%) tumor specimens and 4/6 (66.7%) cell lines. By cloning and sequencing of the 700 bp band, we found that this variant is identical to the CD44 variant sharing only exon v10. Examination by Northern blot analysis has revealed that all tumors express a higher level of CD44 mRNA than paired normal kidneys. These findings suggested that the CD44 variants sharing exon v10 play some role in renal cancer.

[Expression of CD44 variant form in human renal cell carcinoma].

To examine whether renal cell carcinoma display altered CD44 expression we performed reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of CD44 in the tissues resected from 19 patients with renal cell carcinoma and 6 renal cancer cell lines. To detect the CD44 variants, we utilized the RT-PCR Southern blot method reported by Matsumura et al. In 12 of 17 (70.6%) cases, about a 700 base pairs band was emphasized in cancerous tissues compared with normal kidney. Moreover, we found that this isoform is the CD44 variant sharing only exon v10. Examination by Northern blot analysis has revealed that all tumors express a higher level of CD44 variants sharing exon v10. Our findings suggest that this variant form plays some roles in renal cell carcinoma.

Interferon-gamma induces different subunit organizations and functional diversity of proteasomes.

To obtain information on the role of proteasomes in the immune system, we examined the effect of a major immunomodulatory cytokine, gamma interferon (IFN-gamma), on the expressions, structures, and functions of proteasomes. IFN-gamma greatly increased the levels of the mRNAs encoding LMP2 and LMP7, putative immuno-proteasome subunits encoded by genes within the class II MHC region, and these two subunits synthesized were assembled completely into the proteasomal multi-subunit complex in various types of human cells. The subunit organization of proteasome changed in response to IFN-gamma stimulation, due to assembly of newly synthesized subunits through up- and down-expressions of at least 6 proteasome genes including LMP2/LMP7 without change in the structure of pre-existing proteasomes. Interestingly, IFN-gamma dramatically stimulated the trypsin-like and chymotrypsin-like activities of the multifunctional proteasome and depressed the peptidylglutamyl-peptide-hydrolyzing activity, without affecting the activity for ATP-, ubiquitin-dependent proteolysis. These results indicate that IFN-gamma modifies not only the structural organization of the proteasome, but also its functions. Based on these findings, we discuss the role in the antigen processing/presentation pathway of proteasomes with functional diversity acquired through alteration of their subunit assembly in response to IFN-gamma stimulation.

[Jean-Martin Charcot, his time and Kinnosuke Miura].

Jean-Martin Charcot died unexpectedly on August 16, 1893, just 100 years ago. As a young physician, he worked in pathology and medicine under Rayer for nine years, and wrote on diseases of the heart, lungs and kidneys, on rheumatism and gout. In 1862, he was appointed as medical superintendent to the Hospice de la Salpêtrière. This institution contained a population of over 5000 persons, affected with every kind of chronic maladies, particularly with diseases of the nervous system. As a clinician, he could begin to observe, examine and describe with his excellent master Duchenne de Boulogne. And as an anatomo-pathologist in his association with his colleague Vulpian, he devoted himself to compare the pathological findings with clinical records, to bring order into this chaos. During the 8-year period from 1862 to 1870, Charcot made many discoveries that made firm basis for neurology as one new specialty in medicine. The first beautiful fruit of their effort was the clinico-pathological identification of "Disseminated Sclerosis", clearly differentiated from "Paralysis agitans" of which first reported by Parkinson 1817. He succeeded Vulpian to the chair of pathological anatomy in 1872. The sequential decrement of the shift may be a reason why the disease is self-limited.(ABSTRACT TRUNCATED AT 250 WORDS)

[Gene expressions of type IV collagenase and tissue inhibitor of metalloproteinases (TIMP) in human bladder cancers].

Matrixmetalloproteinases (MMP), such as type IV collagenases and interstitial collagenases, play an important role in tumor invasion and metastasis. And tissue inhibitor of metalloproteinases (TIMP) inhibit collagenolytic activity of these enzymes. We investigated the gene expressions of MMP-9 (92 kDa type IV collagenase), MMP-2 (72 kDa type IV collagenase), TIMP-1 and TIMP-2 in bladder cancers by Northern blot and slot blot hybridization. The mRNA levels of MMP-2, TIMP-1 and TIMP-2 increased in the cases with invasion and metastasis of bladder cancers. These findings suggest that MMP-2 acts as a regulator of the invasion and metastasis of bladder cancers. The MMP-2/TIMP-2 ratio increased as tumor invasion and metastasis progressed, suggesting that an imbalance in the MMP and TIMP ratio promote the invasion and metastasis of bladder cancers. And we also investigated the gene expressions of c-fos that activate the collagenase genes, and there was a correlation between c-fos and MMP-2 in gene expressions. It is suggested that fos gene may play an important role for the invasion and metastasis in bladder cancers.

[Thromboangiitis obliterans of the spermatic cord: a case report].

A 61-year-old man was admitted to our hospital with a 8-day history of dull pain and swelling in the bilateral scrotum and bilateral inguinal region. Neoplasma of the spermatic cord was suspected, and bilateral orchiectomy was immediately carried out. Histopathological examination revealed thromboangiitis obliterans of the spermatic cord. The differential diagnosis of a tumorous lesion in the inguinal region is frequently difficult. In such a case, surgical exploration is recommended, and a biopsy must be performed for a definitive diagnosis, but orchiectomy should not be readily selected. Our patient is the first reported case in Japan to our knowledge.