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1.
Curr Opin Genet Dev ; 86: 102201, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38701672

ABSTRACT

Polycomb-associated chromatin and pericentromeric heterochromatin form genomic domains important for the epigenetic regulation of gene expression. Both Polycomb complexes and heterochromatin factors rely on 'read and write' mechanisms, which, on their own, are not sufficient to explain the formation and the maintenance of these epigenetic domains. Microscopy has revealed that they form specific nuclear compartments separated from the rest of the genome. Recently, some subunits of these molecular machineries have been shown to undergo phase separation, both in vitro and in vivo, suggesting that phase separation might play important roles in the formation and the function of these two kinds of repressive chromatin. In this review, we will present the recent advances in the field of facultative and constitutive heterochromatin formation and maintenance through phase separation.


Subject(s)
Chromatin , Epigenesis, Genetic , Heterochromatin , Polycomb-Group Proteins , Heterochromatin/genetics , Heterochromatin/metabolism , Polycomb-Group Proteins/genetics , Polycomb-Group Proteins/metabolism , Chromatin/genetics , Chromatin/metabolism , Animals , Humans , Histones/genetics , Histones/metabolism , Chromatin Assembly and Disassembly/genetics , Phase Separation
2.
Proc Natl Acad Sci U S A ; 119(29): e2122026119, 2022 07 19.
Article in English | MEDLINE | ID: mdl-35858337

ABSTRACT

Hosts are continually selected to evolve new defenses against an ever-changing array of pathogens. To understand this process, we examined the genetic basis of resistance to the Drosophila A virus in Drosophila melanogaster. In a natural population, we identified a polymorphic transposable element (TE) insertion that was associated with an ∼19,000-fold reduction in viral titers, allowing flies to largely escape the harmful effects of infection by this virulent pathogen. The insertion occurs in the protein-coding sequence of the gene Veneno, which encodes a Tudor domain protein. By mutating Veneno with CRISPR-Cas9 in flies and expressing it in cultured cells, we show that the ancestral allele of the gene has no effect on viral replication. Instead, the TE insertion is a gain-of-function mutation that creates a gene encoding a novel resistance factor. Viral titers remained reduced when we deleted the TE sequence from the transcript, indicating that resistance results from the TE truncating the Veneno protein. This is a novel mechanism of virus resistance and a new way by which TEs can contribute to adaptation.


Subject(s)
DNA Transposable Elements , Dicistroviridae , Drosophila melanogaster , Host-Pathogen Interactions , Tudor Domain , Animals , DNA Transposable Elements/genetics , Drosophila melanogaster/genetics , Drosophila melanogaster/virology , Gain of Function Mutation , Host-Pathogen Interactions/genetics , Sequence Deletion
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