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1.
Oncogene ; 34(27): 3556-67, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25195861

ABSTRACT

The serine protease inhibitor, elafin, is a critical component of the epithelial barrier against neutrophil elastase (NE). Elafin is downregulated in the majority of breast cancer cell lines compared with normal human mammary epithelial cells (HMECs). Here, we evaluated the role of elafin and NE on proliferation and tumorigenesis. Elafin is induced in growth factor-deprived HMECs as they enter a quiescent (G0) state, suggesting that elafin is a counterbalance against the mitogenic effects of NE in G0 HMECs. Stable knockdown of elafin compromises the ability of HMECs to maintain G0 arrest during long-term growth factor deprivation; this effect can be reversed by re-expression of wild-type elafin but not elafin-M25G lacking protease inhibitory function. These results suggest that NE, which is largely contributed by activated neutrophils in the tumor microenvironment, may be negatively regulating the ability of elafin to arrest cells in G0. In fact when purified NE was added to elafin-knocked down HMECs, these cells demonstrated greater sensitivity to the growth-promoting effects of purified NE. Activation of ERK signaling, downstream of toll-like receptor 4, was essential to the mitogenic effect of NE on HMECs. These findings were next translated to patient samples. Immunohistochemical analysis of normal breast tissue revealed robust elafin expression in the mammary epithelium; however, elafin expression was dramatically downregulated in a significant proportion of human breast tumor specimens. The loss of elafin expression during breast cancer progression may promote tumor growth as a consequence of increased NE activity. To address the role of NE in mammary tumorigenesis, we next examined whether deregulated NE activity enhances mammary tumor growth. NE knockout in the C3(1)TAg mouse model of mammary tumorigenesis suppressed proliferation and reduced the kinetics of tumor growth. Overall, the imbalance between NE and its inhibitors, such as elafin, presents an important therapeutic target in breast cancer.


Subject(s)
Cell Proliferation/genetics , Elafin/physiology , Leukocyte Elastase/antagonists & inhibitors , Leukocyte Elastase/physiology , Animals , Breast Neoplasms/genetics , Breast Neoplasms/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Elafin/genetics , Female , Gene Knockdown Techniques , Humans , Leukocyte Elastase/pharmacology , MCF-7 Cells , Mice , Mice, Inbred C57BL , Mice, Transgenic , Mitosis/drug effects , Mitosis/genetics
2.
Oncogene ; 29(27): 3896-907, 2010 Jul 08.
Article in English | MEDLINE | ID: mdl-20453888

ABSTRACT

HER2/neu (HER2) and cyclin E are important prognostic indicators in breast cancer. As both are involved in cell cycle regulation we analyzed whether there was a direct interaction between the two. HER2 and cyclin E expression levels were determined in 395 breast cancer patients. Patients with HER2-overexpression and high levels of cyclin E had decreased 5-year disease-specific survival compared with low levels of cyclin E (14% versus 89%, P<0.0001). In vitro studies were performed in which HER2-mediated activity in HER2-overexpressing breast cancer cell lines was downregulated by transfection with HER2 small interfering RNA or treatment with trastuzumab. Cyclin E expression levels were determined by western blot analysis, and functional effects analyzed using kinase assays, MTT assays were used to assess cell viability as a marker of proliferation and fluorescence-activated cell sorting analysis was used to determine cell cycle profiles. Decreased HER2-mediated signaling resulted in decreased expression of cyclin E, particularly the low molecular weight (LMW) isoforms. Decreased HER2 and LMW cyclin E expression had functional consequences, including decreased cyclin E-associated kinase activity and decreased proliferation, because of increased apoptosis and an increased accumulation of cells in the G1 phase. In vivo studies performed in a HER2-overexpressing breast cancer xenograft model confirmed the effects of trastuzumab on cyclin E expression. Given the relationship between HER2 and cyclin E, in vitro clonogenic assays were performed to assess combination therapy targeting both proteins. Isobologram analysis showed a synergistic interaction between the two agents (trastuzumab targeting HER2 and roscovitine targeting cyclin E). Taken together, these studies show that HER2-mediated signaling effects LMW cyclin E expression, which in turn deregulates the cell cycle. LMW cyclin E has prognostic and predictive roles in HER2-overexpressing breast cancer, warranting further study of its potential as a therapeutic target.


Subject(s)
Breast Neoplasms/metabolism , Cyclin E/metabolism , Receptor, ErbB-2/metabolism , Animals , Antibodies, Monoclonal/pharmacology , Antibodies, Monoclonal/therapeutic use , Antibodies, Monoclonal, Humanized , Breast Neoplasms/drug therapy , Breast Neoplasms/genetics , Breast Neoplasms/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Cyclin E/chemistry , Cyclin E/genetics , Down-Regulation , G1 Phase/drug effects , Gene Expression Regulation, Neoplastic , Humans , Mice , Mice, Nude , Molecular Weight , Protein Binding , Signal Transduction/drug effects , Survival Rate , Transcription, Genetic , Trastuzumab
3.
Circ Res ; 85(4): 319-28, 1999 Aug 20.
Article in English | MEDLINE | ID: mdl-10455060

ABSTRACT

E1A can evoke G1 exit in cardiac myocytes and other cell types by displacing E2F transcription factors from tumor suppressor "pocket" proteins and by a less well-characterized p300-dependent pathway. Bypassing pocket proteins (through overexpression of E2F-1) reproduces the effect of inactivating pocket proteins (through E1A binding); however, pocket proteins associate with a number of molecular targets apart from E2F. Hence, pocket protein binding by E1A might engage mechanisms for cell cycle reentry beyond those induced by E2F-1. To test this hypothesis, we used adenoviral gene transfer to express various E2F-1 and E1A proteins in neonatal rat cardiac myocytes that are already refractory to mitogenic serum, in the absence or presence of several complementary cell cycle inhibitors-p16, p21, or dominant-negative cyclin-dependent kinase-2 (Cdk2). Rb binding by E2F-1 was neither necessary nor sufficient for G1 exit, whereas DNA binding was required; thus, exogenous E2F-1 did not merely function by competing for the Rb "pocket." E2F-1-induced G1 exit was blocked by the "universal" Cdk inhibitor p21 but not by p16, a specific inhibitor of Cdk4/6; p21 was permissive for E2F-1 induction of cyclins E and A, but prevented their stimulation of Cdk2 kinase activity. In addition, E2F-1-induced G1 exit was blocked by dominant-negative Cdk2. Forced expression of cyclin E induced endogenous Cdk2 activity but not G1 exit. Thus, E2F-1-induced Cdk2 function was necessary, although not sufficient, to trigger DNA synthesis in cardiac muscle cells. In contrast, pocket protein-binding forms of E1A induced G1 exit that was resistant to inhibition by p21, whereas G1 exit via the E1A p300 pathway was sensitive to inhibition by p21. Both E1A pathways-via pocket proteins and via p300-upregulated cyclins E and A and Cdk2 activity, consistent with a role for Cdk2 in G1 exit induced by E1A. However, p21 blocked Cdk2 kinase activity induced by both E1A pathways equally. Thus, E1A can cause G1 exit without an increase in Cdk2 activity, if the pocket protein-binding domain is intact. E1A also overrides p21 in U2OS cells, provided the pocket protein-binding domain is intact; thus, this novel function of E1A is not exclusive to cardiac muscle cells. In summary, E1A binding to pocket proteins has effects beyond those produced by E2F-1 alone and can drive S-phase entry that is resistant to p21 and independent of an increase in Cdk2 function. This suggests the potential involvement of other endogenous Rb-binding proteins or of alternative E1A targets.


Subject(s)
Adenovirus E1A Proteins/physiology , CDC2-CDC28 Kinases , Cell Cycle/physiology , Cyclin-Dependent Kinases/physiology , Cyclins/physiology , Myocardium/cytology , Protein Serine-Threonine Kinases/physiology , Animals , Cells, Cultured , Cyclin-Dependent Kinase 2 , Cyclin-Dependent Kinase Inhibitor p21 , Enzyme Activation , Gene Expression Regulation/physiology , Gene Transfer Techniques , Heart/physiology , Rats , Rats, Sprague-Dawley , Signal Transduction
4.
Hum Mol Genet ; 7(5): 831-8, 1998 May.
Article in English | MEDLINE | ID: mdl-9536087

ABSTRACT

Tay-Sachs disease is a severe neurodegenerative disorder due to mutations in the HEXA gene coding for the alpha-chain of the alpha-beta heterodimeric lysosomal enzyme beta-hexosaminidase A (HexA). Because no treatment is available for this disease, we have investigated the possibility of enzymatic correction of HexA-deficient cells by HEXA gene transfer. Human HEXA cDNA was subcloned into a retroviral plasmid generating to G.HEXA vector. The best Psi-CRIP producer clone of G.HEXA retroviral particles was isolated, and murine HexA-deficient fibroblasts derived from hexa -/- mice were transduced with the G.HEXA vector. Transduced cells overexpressed the alpha-chain, resulting in the synthesis of interspecific HexA (human alpha-chain/murine beta-chain) and in a total correction of HexA deficiency. The alpha-chain was secreted in the culture medium and taken up by HexA-deficient cells via mannose-6-phosphate receptor binding, allowing for the restoration of intracellular HexA activity in non-transduced cells.


Subject(s)
Tay-Sachs Disease/enzymology , Tay-Sachs Disease/genetics , Transfection , beta-N-Acetylhexosaminidases/genetics , 3T3 Cells , Animals , Cloning, Molecular , Enzyme Activation/drug effects , Enzyme Activation/genetics , Fibroblasts/metabolism , Gene Expression Regulation, Enzymologic , Gene Transfer Techniques , Genetic Vectors/genetics , Hexosaminidase A , Humans , Isoenzymes/metabolism , Mice , Mice, Inbred C57BL , Retroviridae/genetics , beta-N-Acetylhexosaminidases/metabolism
5.
J Neurosci ; 17(19): 7228-36, 1997 Oct 01.
Article in English | MEDLINE | ID: mdl-9295369

ABSTRACT

Synthesis of the ciliary neurotrophic factor (CNTF) and its specific receptor (CNTFRalpha) is widespread in the intact CNS, but potential biological roles for this system remain elusive. Contradictory results have been obtained concerning a possible effect on the morphological and biochemical phenotype of astrocytes. To reassess this question, we have taken advantage of adenovirus-mediated gene transfer into the rat brain to obtain the local release of CNTF. Stereotaxic administration of CNTF recombinant adenovirus vectors into the striatum led to phenotypic changes in astrocytes located in regions that were related axonally to striatal neurons at the injection site. Astrocytes appeared hypertrophied and displayed an increase in both GFAP and CNTF immunoreactivity. This response was observed up to 5 weeks after injection, the longest time studied. It was not observed after the administration of a control vector. The methodology used in the present study, allowing us to analyze the effect of the factor in areas remote from the injection site, has provided conclusive evidence that CNTF affects the astroglial phenotype in the intact CNS. The characteristics of these effects may explain why contradictory results have been obtained previously, because this signaling system seems to have a low efficiency and therefore requires a high local concentration of the factor close to the target cells. One might speculate as to the involvement of a CNTF astroglio-astroglial signaling system in the organized response of a population of astrocytes to changes in CNS homeostasis detected locally, even by a single cell.


Subject(s)
Adenoviridae/genetics , Astrocytes/physiology , Brain/drug effects , Gene Transfer Techniques , Genetic Vectors , Nerve Tissue Proteins/pharmacology , Animals , Astrocytes/cytology , Astrocytes/drug effects , Brain/cytology , Brain/metabolism , Cell Differentiation , Ciliary Neurotrophic Factor , Injections , Nerve Tissue Proteins/genetics , Phenotype , Rats , Rats, Sprague-Dawley
6.
Nat Med ; 3(4): 429-36, 1997 Apr.
Article in English | MEDLINE | ID: mdl-9095177

ABSTRACT

Motor neuron diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy cause progressive paralysis, often leading to premature death. Neurotrophic factors have been suggested as therapeutic agents for motor neuron diseases, but their clinical use as injected recombinant protein was limited by toxicity and/or poor bioavailability. We demonstrate here that adenovirus-mediated gene transfer of neurotrophin-3 (NT-3) can produce substantial therapeutic effects in the mouse mutant pmn (progressive motor neuronopathy). After intramuscular injection of the NT-3 adenoviral vector, pmn mice showed a 50% increase in life span, reduced loss of motor axons and improved neuromuscular function as assessed by electromyography. These results were further improved by coinjecting an adenoviral vector coding for ciliary neurotrophic factor. Therefore, adenovirus-mediated gene transfer of neurotrophic factors offers new prospects for the treatment of motor neuron diseases.


Subject(s)
Genetic Therapy/methods , Motor Neuron Disease/therapy , Nerve Growth Factors/therapeutic use , Nerve Tissue Proteins/therapeutic use , Adenoviridae/genetics , Animals , Animals, Newborn , Ciliary Neurotrophic Factor , Electromyography , Genetic Vectors , Injections, Intramuscular , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Mutant Strains , Motor Neuron Disease/mortality , Muscles/innervation , Nerve Degeneration/drug effects , Nerve Growth Factors/genetics , Nerve Tissue Proteins/genetics , Neurotrophin 3 , Phrenic Nerve/pathology , Survival Analysis
7.
Gene Ther ; 3(9): 769-74, 1996 Sep.
Article in English | MEDLINE | ID: mdl-8875224

ABSTRACT

Tay-Sachs disease (TSD) is a lysosomal storage disease due to hexosaminidase A deficiency caused by mutations in the gene for alpha-chain (Hex alpha). A human Hex alpha cDNA was subcloned into the adenoviral plasmid pAdRSV. Hex alpha. Replication-deficient adenovirus was generated by homologous recombination in 293 cells. Human fibroblasts from a patient suffering from TSD were infected with the recombinant adenovirus. TSD fibroblasts expressing the recombinant alpha-chain had an enzyme activity on the natural substrate ranging from 40 to 84% of the normal. The corrected cells secreted up to 25 times more Hex alpha than control fibroblasts. The Hex alpha encoded by the adenovirus was shown to be correctly transported into the lysosomes and to normalize the impaired degradation of GM2 ganglioside in TSD fibroblasts.


Subject(s)
Adenoviridae/genetics , Fibroblasts/enzymology , Gene Transfer Techniques , Genetic Vectors/genetics , Tay-Sachs Disease/genetics , beta-N-Acetylhexosaminidases/genetics , DNA, Complementary/genetics , G(M2) Ganglioside/metabolism , Gene Expression , Hexosaminidase A , Humans , Lysosomes/enzymology , RNA, Messenger/analysis , Tay-Sachs Disease/enzymology , beta-N-Acetylhexosaminidases/metabolism
8.
C R Seances Soc Biol Fil ; 190(1): 9-11, 1996.
Article in French | MEDLINE | ID: mdl-8881264

ABSTRACT

In hereditary neurological diseases, gene transfer into neurons is made difficult by: the nature of the cells (postmitotic cells, that cannot be cultured, genetically modified ex vivo, then retransplanted), sometimes, their widespread localization, the blood-brain barrier. However, three viral vectors derived from adenovirus, Herpes simplex virus and adeno-associated virus have been shown to be very efficient in transferring DNA into brain cells. All of these vectors can infect resting cells, especially neurons, and are efficient in vivo. Retroviral vectors which can infect dividing cells only are mainly used for ex vivo genetic modification of cells (neural progenitor cells, myoblasts, fibroblasts) followed by intracerebral transplantation. Alternatively, genetically modified cells can be transplanted in a peripheral site if the transgene product is able to cross the blood-brain barrier or to be transported retrogradely from the nerve terminals. We have especially investigated the potential interest of adenoviral vectors to transfer foreign genes into brain cells and to treat animal models of neurological diseases. These vectors allowed us to transfer the lacZ gene into any neural cell type, including neurons, glia, photoreceptors and olfactory receptors, ex vivo, in cell culture, and in vivo, by stereotactic administration. In addition, axonal transport of adenoviral vectors has been demonstrated, e.g. in the substantia nigra after injection into the striatum, in the olfactory bulb after intranasal instillation and in spinal motor neurons after intramuscular injection. After intracerebroventricular injection, ependymal cells are massively infected and express the transgene for several months, as this is also observed in neurons. Through the spinal canal and cerebrospinal fluid, the vector can diffuse to a considerable distance from the injection point, e.g. to the lumbar spinal cord after injection in the suboccipital region. To test the biological function of transgenes transferred through adenoviral vectors, we have constructed vectors with cDNAs or genes for various neutrophic factors: CNTF, NT3, BDNF and GDNF. These vectors were biologically active on target cells, ex vivo and in vivo. In the pmn mouse model of progressive motor neuronal degeneration, some of these vectors, alone or combined, allowed for prolongation of life of homozygous animals by more than two fold, and for decrease in the demyelination of phrenic nerve axons. Finally, we have also constructed an adenoviral vector carrying the alpha-hexosaminidase cDNA, encoding the enzyme subunit deficient in Tay Sachs patients. This vector permitted to normalize ganglioside metabolism in Tay Sachs fibroblasts and is currently tested in knock out mice deficient in hexosaminidase A. In spite of all these encouraging results, we are nevertheless aware that progress in vector design and delivery strategies will be needed before gene therapy can become a realistic therapeutical strategy in humans.


Subject(s)
Genetic Therapy , Hereditary Sensory and Autonomic Neuropathies/therapy , Adenoviridae/genetics , Animals , Genetic Vectors
9.
Mamm Genome ; 6(12): 844-9, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8747922

ABSTRACT

Tay-Sachs disease is an autosomal recessive lysosomal storage disease caused by beta-hexosaminidase A deficiency and leads to death in early childhood. The disease results from mutations in the HEXA gene, which codes for the alpha chain of beta-hexosaminidase. The castastrophic neurodegenerative progression of the disease is thought to be a consequence of massive neuronal accumulation of GM2 ganglioside and related glycolipids in the brain and nervous system of the patients. Fuller understanding of the pathogenesis and the development of therapeutic procedures have both suffered from the lack of an animal model. We have used gene targeting in embryonic stem (ES) cells to disrupt the mouse Hexa gene. Mice homozygous for the disrupted allele mimic several biochemical and histological features of human Tay-Sachs disease. Hexa-/- mice displayed a total deficiency of beta-hexosaminidase A activity, and membranous cytoplasmic inclusions typical of GM2 gangliosidoses were found in the cytoplasm of their neurons. However, while the number of storage neurons increased with age, it remained low compared with that found in human, and no apparent motor or behavioral disorders could be observed. This suggests that the presence of beta-hexosaminidase A is not an absolute requirement of ganglioside degradation in mice. These mice should help us to understand several aspects of the disease as well as the physiological functions of hexosaminidase in mice. They should also provide a valuable animal model in which to test new forms of therapy, and in particular gene delivery into the central nervous system.


Subject(s)
Lysosomal Storage Diseases/genetics , beta-N-Acetylhexosaminidases/genetics , Animals , Base Sequence , Brain/metabolism , Brain/pathology , Cell Line , DNA Primers , Female , Hexosaminidase A , Male , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Neurons/metabolism , Phenotype , RNA/genetics , Reproduction , Tay-Sachs Disease/genetics , beta-N-Acetylhexosaminidases/deficiency , beta-N-Acetylhexosaminidases/metabolism
11.
Neuroreport ; 5(9): 1069-72, 1994 May 09.
Article in English | MEDLINE | ID: mdl-8080960

ABSTRACT

Adenovirus vectors containing a marker gene (lacZ from Escherichia coli) are potent for transferring the gene to neurones after intraparenchymal injections. Expression of the marker gene may lead to the synthesis of an enormous amount of beta-galactosidase which diffuses throughout the entire neurone, providing a 'Golgi-like' staining. This suggested that the technique may be used to study the morphology of specific neuronal populations. We have validated this hypothesis by analysing the postnatal development of motoneurones in the rat cervical cord. Injections of the viral suspension into one ventral horn were performed at different ages after birth. Histochemical staining using X-Gal revealed morphological changes occurring within the first 3 weeks with enlargement of the perikaryon and increased dendritic complexity. Immunoreactivity for CGRP was visualized in double-staining experiments. In vivo transfer of a marker gene therefore provides a new way to analyse neuronal morphology which allows selection of the cells to be studied and double-labelling with immunohistochemical markers.


Subject(s)
Gene Transfer Techniques , Motor Neurons/physiology , Spinal Cord/growth & development , Adenoviridae/genetics , Animals , Calcitonin Gene-Related Peptide/immunology , Calcitonin Gene-Related Peptide/metabolism , Dendrites/ultrastructure , Genetic Markers/immunology , Genetic Vectors , Immunohistochemistry , Rats , Rats, Sprague-Dawley , Spinal Cord/cytology , Spinal Cord/enzymology , beta-Galactosidase/genetics , beta-Galactosidase/metabolism
12.
Biomed Pharmacother ; 48(8-9): 341-6, 1994.
Article in English | MEDLINE | ID: mdl-7858168

ABSTRACT

The abnormalities in the gene coding for the beta-hexosaminidase alpha subunit were analysed from fibroblast's RNAs of 42 Tay-Sachs patients (seven with adult or late onset of Tay-Sachs disease and 35 with infantile Tay-Sachs disease). After first strand synthesis by random priming, PCR was used to amplify in two overlapping fragments (868 and 949 bp) the entire coding region. These amplified products were first studied for changes in size by agarose gel electrophoresis to screen for splicing mutations leading to exon skipping or cryptic splice site activation. For each patient, the two overlapping cDNA fragments were subjected to chemical mismatch cleavage analysis using hydroxylamine to modify C-containing mismatches and osmium tetroxide to modify T-containing mismatches. DGGE was used to screen for mutations in the coding region spanning exon 2 to exon 6, a region putatively encompassing the active site and therefore a potential hot spot of mutations associated with Tay-Sachs disease. To increase the sensitivity of the technique, a 30 bp GC-clamp has been added at the 5' end of the sense oligonucleotide to amplify a fragment of 629 bp. The computerized analysis found that single base changes in domain spanning from nt 313 to nt 693 can be distinguished. Fragments displaying an altered melting behavior or a cleaved product were further analysed by direct sequencing of the amplified material. These methods as a whole allowed us to identify 30/38 alleles studied (79%) with 15 point mutations and one 4 bp insertion detected.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Tay-Sachs Disease/epidemiology , Tay-Sachs Disease/genetics , Adult , Base Sequence , Electrophoresis, Polyacrylamide Gel , Europe/epidemiology , Genotype , Humans , Infant , Mutation , Polymerase Chain Reaction , Tay-Sachs Disease/ethnology
13.
Eur J Neurosci ; 5(10): 1287-91, 1993 Oct 01.
Article in English | MEDLINE | ID: mdl-8275231

ABSTRACT

Previous studies have demonstrated that a defective recombinant adenovirus can infect a wide range of postmitotic and slowly proliferating cell types such as hepatocytes, myotubes, pneumocytes and intestinal cells (Stratford-Perricaudet et al., Hum. Gene Ther., 1, 241-256, 1990; Quantin et al., Proc. Natl. Acad. Sci. USA, 89, 2581-2584, 1992; Jaffe et al., Nature Genetics, 1, 372-378, 1992). We have used a defective recombinant adenovirus, Ad.RSV beta gal, containing the Escherichia coli beta-galactosidase gene targeted to the nucleus under the transcriptional control of the Rous sarcoma virus long terminal repeat promoter (Stratford-Perricaudet et al., J. Clin. Invest., 90, 626-630, 1992) to infect non-dividing neural cells in primary culture. We show that 80-100% of neuronal and astroglial cells infected with a viral titre lower than 10(9) p.f.u./ml express beta-galactosidase for at least 1 month without cell damage. These results demonstrate the potential usefulness of recombinant adenovirus infection for the analysis of brain-specific gene regulation and for the transfer of genes into neural cells before their transplantation into the brain.


Subject(s)
Avian Sarcoma Viruses/genetics , Brain/physiology , Genetic Vectors , Neuroglia/physiology , Neurons/physiology , Transfection , beta-Galactosidase/biosynthesis , Animals , Cell Nucleus/enzymology , Cells, Cultured , Embryo, Mammalian , Escherichia coli/genetics , Mice , Promoter Regions, Genetic , Rats , Recombinant Proteins/analysis , Recombinant Proteins/biosynthesis , Repetitive Sequences, Nucleic Acid , beta-Galactosidase/analysis
15.
Nat Genet ; 3(3): 224-8, 1993 Mar.
Article in English | MEDLINE | ID: mdl-8485577

ABSTRACT

The ability of a replication-deficient adenovirus vector to transfer a foreign gene into neural cells of adult rats in vivo has been analysed. A large number of neural cells (including neurons, astrocytes and ependymal cells) expressed an E. coli lacZ transgene for at least 45 days after inoculation of various brain areas. Injecting up to 3 x 10(5) pfu in 10 microliters did not result in any detectable cytopathic effects--these were only observed for very high titres of infection (> 10(7) pfu 10 microliters-1). Adenovirus vectors therefore appear to be a promising means for in vivo transfer of therapeutic genes into the central nervous system.


Subject(s)
Adenoviridae/genetics , Brain/cytology , Genes, Bacterial , Neurons/cytology , Transfection/methods , beta-Galactosidase/metabolism , Animals , Brain/enzymology , Escherichia coli/enzymology , Escherichia coli/genetics , Genetic Therapy/methods , Genetic Vectors , Golgi Apparatus/enzymology , Golgi Apparatus/ultrastructure , Neurons/enzymology , Rats , Rats, Sprague-Dawley , Recombination, Genetic , beta-Galactosidase/analysis , beta-Galactosidase/genetics
16.
Hum Genet ; 90(6): 614-20, 1993 Feb.
Article in English | MEDLINE | ID: mdl-8444467

ABSTRACT

The molecular basis of null alleles was investigated by cDNA polymerase chain reaction (PCR) in seven Tay-Sachs patients. Although mRNAs were undetectable by Northern blot, cDNA-PCR amplification allowed us to get a sufficient amount of cDNA to characterize abnormal transcripts. In two French patients (one homozygote and one compound heterozygote with a 4-bp insertion in exon 11 of the second allele) suffering an infantile form of the disease, we detected abnormal RNAs with a 17-bp insertion due to a GT to AT transition at the donor site of intron 9, resulting in the activation of a cryptic donor site in the intron. This mutation has been found in 9 out of 82 Tay-Sachs chromosomes (11%) in association with alleles responsible from different clinical courses. In the other five patients we found the 4-bp insertion in exon 11 and two nonsense mutations.


Subject(s)
Alleles , Tay-Sachs Disease/genetics , Base Sequence , Blotting, Northern , DNA Mutational Analysis , Exons , Female , Frameshift Mutation , Humans , Infant , Introns , Male , Molecular Sequence Data , Pedigree , Point Mutation , Polymerase Chain Reaction , RNA Splicing , RNA, Messenger/analysis
17.
Hum Mol Genet ; 2(1): 61-7, 1993 Jan.
Article in English | MEDLINE | ID: mdl-8490625

ABSTRACT

The heterogeneity of mutations causing Tay-Sachs disease in non-Jewish populations requires efficient techniques allowing the simultaneous screening for both known and novel mutations. beta-hexosaminidase mRNA isolated from cultured fibroblasts of 19 Tay-Sachs patients (7 with adult or late onset form of the disease and 12 with infantile Tay-Sachs disease) was amplified by cDNA-PCR in two overlapping segments spanning the entire coding sequence. We used chemical mismatch cleavage (CMC), denaturing gradient gel electrophoresis (DGGE) and direct sequencing of amplified fragments displaying a cleaved product or an altered melting behavior to screen the HEX A gene for mutations and to determine their distribution and frequency in the non-Jewish Tay-Sachs patients. These methods allowed us to identify 31 out of 38 alleles studied (82%). In addition to 9 previously described mutations (the 4 bp insertion in exon 11, G to A transitions at codons 170, 269, 482, 499 and 504, C to T transition at codon 499 and 504 and a GT to AT transition at the donor site of intron 9), we have identified 10 novel mutations. These include 1 donor splice site defect in intron 6, 8 missense mutations at non-randomly distributed conserved residues and a 2 bp deletion in exon 4. These results confirm the extreme molecular heterogeneity of mutations causing Tay-Sachs disease in non-Jewish population. The strategy used should be profitable for identifying mutations in large genes and for diagnostic purposes.


Subject(s)
Point Mutation , Tay-Sachs Disease/enzymology , Tay-Sachs Disease/genetics , beta-N-Acetylhexosaminidases/genetics , Adult , Amino Acid Sequence , Base Sequence , Cells, Cultured , Ethnicity , Exons , Fibroblasts/enzymology , Gene Frequency , Hexosaminidase A , Humans , Infant , Introns , Jews , Molecular Sequence Data , Oligodeoxyribonucleotides , Polymerase Chain Reaction/methods , RNA, Messenger/genetics , RNA, Messenger/isolation & purification
18.
Eur J Hum Genet ; 1(3): 229-38, 1993.
Article in English | MEDLINE | ID: mdl-8044648

ABSTRACT

Tay-Sachs disease is a lipidosis due to the deficiency of the lysosomal hexosaminidase A. In order to understand the molecular mechanisms of this enzyme deficiency we studied 42 patients of different ethnic origins diagnosed in Europe. The strategy used consists in HEXA cDNA amplification followed by allele-specific oligonucleotide analysis for the frequent mutations, and by chemical cleavage mismatch and denaturing gradient gel electrophoresis for the detection of new mutations. 90% of alleles were clarified in this way, showing a high heterogeneity of HEXA lesions in Tay-Sachs disease. 28 different mutations were found, 20 being identified for the first time in this group of patients.


Subject(s)
Mutation , Tay-Sachs Disease/epidemiology , Tay-Sachs Disease/genetics , beta-N-Acetylhexosaminidases/genetics , Adult , Alleles , Base Composition , Base Sequence , Child, Preschool , DNA Mutational Analysis/methods , Dinucleoside Phosphates/genetics , Electrophoresis, Polyacrylamide Gel/methods , Frameshift Mutation , Genotype , Hexosaminidase A , Humans , Infant , Molecular Epidemiology , Molecular Sequence Data , Mutagenesis, Insertional , Nucleic Acid Heteroduplexes/genetics , Oligonucleotide Probes , Phenotype , Point Mutation , RNA Splicing , Sequence Deletion
19.
Clin Genet ; 41(6): 331-4, 1992 Jun.
Article in English | MEDLINE | ID: mdl-1623631

ABSTRACT

Beta-mannosidosis is a recently described inherited disorder with predominantly neurological signs and symptoms as the major manifestations of the disorder. The heterogeneous manifestations of the disease have been presented in seven previous patients. We describe a further case of European descent with an infantile onset of the disease, with the features of speech impairment as the first symptom. Beta-mannosidase activity was completely deficient in the patient and a heterozygote level was found in the parents. In addition, mannosyl-N-acetylglucosamine was identified in the patient's urine in keeping with the diagnosis of beta-mannosidosis.


Subject(s)
Affective Symptoms/etiology , Mannosidases/deficiency , Speech Disorders/etiology , alpha-Mannosidosis/psychology , Child, Preschool , Humans , Male , Oligosaccharides/urine , alpha-Mannosidosis/complications , alpha-Mannosidosis/enzymology , beta-Mannosidase
20.
J Clin Invest ; 88(4): 1161-6, 1991 Oct.
Article in English | MEDLINE | ID: mdl-1918370

ABSTRACT

We have previously demonstrated that there is a low level of transcription of tissue-specific genes in every cell type. In this study, we have taken advantage of this phenomenon, called illegitimate transcription, to analyze the muscle-type dystrophin mRNA in easily accessible cells such as lymphoid cells, fibroblasts, and peripheral blood cells from Duchenne and Becker muscular dystrophies with known internal gene deletion. The results showed that, in the studied regions surrounding the deletions, processing of truncated transcripts is identical in specific (muscle tissue) and in nonspecific cells (lymphoid cells). In Becker cases with out-of-frame deletions, the already described alternatively spliced species found in muscle samples were also found in nonspecific cells. These results demonstrate that illegitimate transcripts are a bona fide version of tissue-specific mRNA, and that they represent a useful material to investigate the qualitative consequences of gene defects at the mRNA level.


Subject(s)
Dystrophin/genetics , Muscular Dystrophies/genetics , Transcription, Genetic , Base Sequence , Cells, Cultured , Humans , Molecular Sequence Data , Mutation , Phenotype , Polymerase Chain Reaction , RNA Splicing , RNA, Messenger/analysis
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