Hydrogeochemical assessment of aquifer salinization in north-eastern Morocco's Gueroaou coastal plain using statistical methods.

The Guerouaou aquifer investigation spanning 280 km2 in Ain Zohra yields promising outcomes, instilling optimism for regional water quality. These analyses were applied to 45 sampling instances from 43 wells, enabling a comprehensive water quality assessment. Groundwater conductivity ranged from medium to high, peaking at 18360 ms/cm2. The conductivity reveals insights about the groundwater's mineralization. Key physiochemical parameters fell within desirable thresholds, bolstering the positive perspective. HCO3- levels spanned 82-420 mg/L, while chloride content ranged from 38 to 5316 mg/L, benefiting water quality. NO3- ions, vital for gauging pollution, ranged from 0 to 260 mg/L, indicating favorable results. Cation concentrations exhibited encouraging variations: Ca2+- 24 to 647 mg/L, Mg2+- 12 to 440 mg/L, Na+- 18 to 2722 mg/L, K+- 1.75 to 28.65 mg/L. These collectively favor water quality. Halite breakdown dominated mineralization, as evidenced by the prevalence of Na-Cl-Na-SO4 facies. Water resource management and local communities need effective management and mitigation strategies to prevent saltwater intrusion.

Trace metals and macrominerals in common seaweeds in the Marchica (a restored lagoon, Mediterranean Sea): Nutritional value and health risk assessment.

This study investigated the contents of macrominerals (Na, K, Ca, Mg and P) and essential trace metals (Fe, Mn, Cu, Zn and Se) in four species of seaweeds (Gracilaria sp., Alsidium corallinum, Caulerpa prolifera, and Chaetomorpha sp.) from Marchica Lagoon. The contents of macrominerals with mean values (% dw) can be sequenced in this descending order, [Ca > Mg > Na > K > P] for Caulerpa prolifera, and the decreasing sequence [K > Ca > Mg > P > Na] for Chaetomorpha sp. In red seaweeds, Gracilaria sp. and Alsidiumcorallinum followed these orders: [K > Ca > Na > Mg > P] and [K > Na > Ca > Mg > P] respectively. The essential trace metals mean values (mg kg-1) followed the decreasing order [Fe > Mn > Zn > Cu > Se] for Alsidiumcorallinum, Chaetomorpha sp. and Gracilaria sp., and the following order [Fe > Mn > Zn > Se > Cu] for Caulerpa prolifera. Based on the calculated recommended dietary allowance (RDA), targeted hazard quotient (THQ) and the hazard index (HI), the studied seaweeds did not pose any health risk for human consumption.

The chemical characterization and its relationship with heavy metals contamination in surface sediment of Marchica Mediterranean Lagoon (North of Morocco).

The sediments were studied according to the granulometric characteristics, the geochemical analyses of the sediments (organic matter (OM), carbonate, and pH), the analyses of heavy metals (HM) characteristics, and their contamination status in the ecological system and the mineralogical analysis of the sediments at 8 sites spread over the Marchica lagoon (NE-Morocco). Our results showed that the opening of the new wide and deep pass affected the spatial distributions of the metals, which were closely related to fine fraction and sediment OM concentration. In the north and southeast lagoon zones presented low concentrations of the HM, fine particles (clay), OM, whereas the south and the center of the lagoon were heavily loaded with HM and OM, corresponding to the trapping zones by fine particles. The results of mineralogy analyses have revealed the predominance of non-clay minerals such as quartz and calcite, and for the clay fractions of sediments showed that they are formed of illite, kaolinite, smectite, and chlorite in highly variable proportions; illite was the most dominant clay in the north-western Marchica lagoon sediments. Therefore, the HMs are fixed by clay colloids having a high cation exchange capacity with smectite-chlorite-kaolinite assembly.

Valorization of Moroccan Bentonite Deposits: "Purification and Treatment of Margin by the Adsorption Process".

The main objective of this work was to contribute to the reduction in the contamination of phenolic compounds contained in margin by an adsorption process on two types of raw bentonite. The margin used in the studies was collected from a semi-modern oil mill located in the Nador-Morocco region. The results of the physico-chemical analyses showed that the effluents of the oil mills showed that they are highly polluted, particularly in terms of the total suspended solids (TSS), chemical oxygen demand (COD), and iron content of around 154.82 (mg/L), and copper content of 31.72 (mg/L). The mineralogy of bentonites studied by X-ray diffraction (XRD) reveals the existence of two types of montmorillonite; theoretically, the diffraction peak (001) of the montmorillonite appears at 15 Å, with a basal spacing that corresponds to a calcium pole, and the diffraction peak (001) appears at 12Å, with a basal spacing that corresponds to a sodium pole. The specific surface area of the bentonite used is characterized by a large specific surface area, varying between 127.62 m2·g-1 and 693.04 m2·g-1, which is due to the presence of hydrated interleaved cations. This surface is likely to increase in aqueous solution depending on the solid/liquid ratio that modulates the degree of hydration. With a high cation exchange capacity (CEC) (146.54 meq/100 g), samples of margin mixed with raw bentonites at different percentages vary between 5% and 100%. The potential of Moroccan bentonite for the phenol adsorption of 9.17 (g/L) from aqueous solutions was investigated. Adsorption tests have confirmed the effectiveness of these natural minerals in reducing phenolic compounds ranging from 8.72% to 76.23% contained in the margin and the efficiency of heavy metal retention through microelements on raw bentonites. The very encouraging results obtained in this work could aid in the application of adsorption for the treatment of margin.

Improved yield, fruit quality, and salt resistance in tomato co-overexpressing LeNHX2 and SlSOS2 genes.

The K+, Na+/H+ antiporter LeNHX2 and the regulatory kinase SlSOS2 are important determinants of salt tolerance in tomato plants and their fruit production ability. In this work, we have analyzed the effects of LeNHX2 and SlSOS2 co-overexpression on fruit production, quality in tomato plants (Solanum lycopersicum L. cv. MicroTom), and analyzed physiological parameters related to salt tolerance. Plants overexpressing LeNHX2, SlSOS2 or both were grown in greenhouse. They were treated with 125 mM NaCl or left untreated and their salt tolerance was analyzed in terms of plant biomass and fruit yield. Under NaCl cultivation conditions, transgenic tomato plants overexpressing either SlSOS2 or LeNHX2 or both grew better and showed a higher biomass compared to their wild-type plants. Proline, glucose and protein content in leaves as well as pH and total soluble solid (TSS) in fruits were analyzed. Our results indicate that salinity tolerance of transgenic lines is associated with an increased proline, glucose and protein content in leaves of plants grown either with or without NaCl. Salt treatment significantly reduced yield, pH and TSS in fruits of WT plants but increased yield, pH and TSS in fruits of transgenic plants, especially those overexpressing both LeNHX2 and SlSOS2. All these results indicate that the co-overexpression of LeNHX2 and SlSOS2 improve yield and fruit quality of tomato grown under saline conditions.

Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubium vulgare L. from 2 different localities of Northeast of Morocco.

Chemical compositions, biological and antioxidant activities of plants are widely affected by several parameters and conditions, such as geographical and climatic conditions, type of extract (aqueous or organic), as well as the polarity of the extracting solvent. Therefore the present study was the first one designed to study the phytochemical composition, the content of polyphenols, tannins and flavonoids, the antioxidant activities and the chemical composition analysis by FTIR spectroscopic of organic (ethanol, methanol, ethyl Acetate, petroleum ether) and aqueous extracts of Marrubium vulgare L. leaves, collected from two different sampling localities in the North-East of Morocco: Oulad Daoud Zkhanine and the Cape Three Forks. A phytochemical screening was carried out by specific coloring and precipitation reactions. The colorimetric method Folin- Ciocalteu was used for the quantification of total phenolic content. The method of aluminum chloride was employed for the quantification of total flavonoid content and the method of vanillin for the determination of tannins. The antioxidant power was evaluated by the DPPH and ABTS methods. The chemical composition of the organic extracts was analyzed by the FTIR spectroscopy method. Depending on the sampling location of M.vulgare L., the type of extract (aqueous or organic), the polarity of the extracting solvent, and the phytochemical screening revealed the presence of the following secondary metabolites: catechic tannins, terpenoids, polyphenols and flavonoids. The total concentrations of total polyphenols, flavonoids and tannins varied respectively between 0.27 ± 0.1 and 86.91 ± 1.22 µg gallic acid equivalents/mg,6.08 ± 0.17 and 33.82 ± 0.90 µg quercetin equivalents/mg and 2.73 ± 1.15 and 252.68 ± 4.50 µg catechin equivalents/mg. The antioxidant activity that was evaluated by DPPH and ABTS method showed that ethanol extract, methanol and ethyl acetate extract had the highest percentages of inhibition, unlike petroleum ether extract. The inhibitory concentrations (IC50) ranged from 324.55 ± 0.66 to 980 ± 0.62 µg/ml for DPPH and from 107.85 ± 0.19 to 890.74 ± 0.17 µg/ml for ABTS. FTIR spectroscopic analysis has revealed different characteristic peak values with various functional groups in the extracts such as amide, alcohol, phenol compounds. In general, the organic and aqueous extracts of M.vulgare L. that were harvested from Oulad Daoud Zkhanin were richer in secondary metabolites, and showed higher concentrations of polyphenol, flavonoids and tannins. In addition, they revealed a higher antioxidant capacity than the extracts of M. vulgare L.from the Cape Three Forks. Overall this study highlighted the potential benefits and richness of M.vulgare L. harvested from the two study areas and suggested it as a potential source of natural antioxidants that could be used in the food and pharmaceutical fields.

Overexpression of LeNHX4 improved yield, fruit quality and salt tolerance in tomato plants (Solanum lycopersicum L.).

The function of the tomato K+, Na+/H+ antiporter LeNHX4 has been analyzed using 35S-driven gene construct for overexpressing a histagged LeNHX4 protein in Solanum lycopersicum L. Compared to wild-type plants, the expression of LeNHX4 was enhanced in most of plants transformed with a gene construct for LeNHX4 overexpression although some plants showed a decreased LeNHX4 expression. Overexpression of LeNHX4 was associated to an increased fruit size while silencing of this gene was related to a decreased fruit size. We have investigated the effect of LeNHX4 overexpression on fruit production and quality and we have also evaluated salt tolerance in two different overexpression lines by measuring proline, protein and glucose concentrations in tomato leaves grown either under control (0 mM NaCl) or saline (125 mM NaCl) conditions. Plants overexpressing LeNHX4 showed a higher amount of fruits than WT plants and accumulated higher contents of sugars and cations (Na+ and K+). The application of 125 mM NaCl, affected negatively fruit production and quality of WT plants. However the transgenic lines overexpressing LeNXH4 increased fruit quality and yield. In relation to salt tolerance, overexpression lines showed higher levels of leaf proline, glucose and proteins under NaCl treatment. The overexpression of LeNHX4 in tomato plants, improved salinity tolerance and increased fruit yield and quality under both normal and salinity stress conditions.

Seasonal variations of heavy metals content in mussels (Mytilus galloprovincialis) from Cala Iris offshore (Northern Morocco).

Heavy metal concentrations of Cd, Cr, Cu, Fe, Ni, Zn, Co, and Pb were investigated in soft tissues of Mytilus galloprovincialis coming from an aquaculture farm in Cala Iris sea of AlHoceima. Mytilus galloprovincialis were collected monthly during the period January to December 2016. The seasonal variations were affected significantly the concentrations of metals (Cd, Fe and Cr) in M. galloprovincialis. The highest heavy metal concentrations were recorded in winter (0.89 mg/kg, 673.2 and 3.330 mg/kg; for Cd, Fe and Cr, respectively) and the lowest values were founded in summer for Cd (0.646 mg/kg), and in autumn for Fe (340.1 mg/kg) and Cr (1.959 mg/kg). A significant effect of seasons on metal concentrations can be attributed to a number of biological and environmental inter-related factors. Data from this study may provide information on the use of M. galloprovincialis as a bioindicator for heavy metals pollution in the Cala Iris Sea.