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1.
Patol Fiziol Eksp Ter ; (1): 11-3, 2006.
Article in Russian | MEDLINE | ID: mdl-16607886

ABSTRACT

The effect of an endotoxin--E. coli liposaccharide (LPS) of serotype 026:B6--on the respiratory splash (RS) of neutrophils and monocytes in peripheral blood of patients with Familial Mediterranean Fever (FMF) was studied. It is shown that FMF patients have a periodic increase (during an attack) and a decrease (in the period of remission) in endotoxin-induced RS of neutrophils and monocytes. LPS stimulates chemotoxis-induced RS of neutrophils and monocytes in patients both in the period of remission and during the attack equally effectively. Iodine-lithium-alpha-dextrin and sodium thiosulfate have a marked anti-endotoxic effect which manifests with quick neutralization of endotoxin activity on RS of monocytes and neutrophils in FMF patients both during the attack and remission.


Subject(s)
Dextrins/pharmacology , Familial Mediterranean Fever/immunology , Granulocytes/drug effects , Lipopolysaccharides/antagonists & inhibitors , Respiratory Burst/drug effects , Thiosulfates/pharmacology , Adolescent , Adult , Escherichia coli/immunology , Female , Humans , Iodine , Lipopolysaccharides/immunology , Lithium , Male , Monocytes/drug effects
2.
Klin Lab Diagn ; (1): 11-5, 2006 Jan.
Article in Russian | MEDLINE | ID: mdl-16512475

ABSTRACT

Microecological "failures" are an important pathogenetic factor of different diseases and, in the authors' opinion, periodic disease (PD) is one of them. PD is a recessive disease characterized by fever attacks and neutrophil-mediated serous inflammation. A genetic factor has been established to be responsible for half the cases of PD, the influence of non-hereditary factors, particularly a role of the host automicroflora in the genesis of an inflammatory process, has been little studied. The authors' early studies indicate that there are changes in the qualitative and quantitative composition of microbial molecules in the blood of patients with PD. The anaerobic bacterial metabolites that are volatile fatty acids (VFAs) represent biologically active substances that affect the growth of the microflora, on the one hand, and the host's immunological responsiveness, on the other. Out of VFAs, only is acetic acid detectable in small quantities in the blood of healthy individuals. The other VFAs, namely propionic, valeric, butyric, and caproic acids and their isomers, are absent. Gas chromatography was used for qualiitative and quantitative determination of the metabolites of anaerobic microorganisms in the blood of patients with PD (n = 13) during an attack and remission and in that of healthy volunteers (Armenians) (n = 5) of a control group from one Yerevan region. The blood samples from all the patients with PD displayed a significantly higher concentration of caproic acid while the latter was absent in the blood of the controls. This finding suggests that there is a specific shift in the structure of the microbiocenosis in patients with PD. It is conceivable that caproic acid plays a certain role in the pathogenesis of the disease under study. Further studies will deal with the association of some microbial molecules with the manifestation of an attack of PD, which may provide the key to the goal-oriented regulation of detected homeostatic disorders and to the management of the frequency of its attacks.


Subject(s)
Familial Mediterranean Fever/blood , Fatty Acids, Volatile/blood , Armenia , Bacteria, Anaerobic/metabolism , Familial Mediterranean Fever/microbiology , Familial Mediterranean Fever/pathology , Female , Fever/blood , Fever/microbiology , Humans , Inflammation/blood , Inflammation/microbiology , Male , Recurrence
3.
Patol Fiziol Eksp Ter ; (4): 11-2, 2006.
Article in Russian | MEDLINE | ID: mdl-17300081

ABSTRACT

We investigated spontaneous, chemotaxis-, phagocytosis- and proteinkinase C-dependent respiratory burst of neutrophils and monocytes in the whole blood of patients with familial Mediterranean fever (FMF). We also analysed transient activation of neutrophils and monocytes on the level of a single cell using flow cytofluorimetry. It is shown that compared to healthy donors, the respiratory burst of monocytes and neutrophils in the patients is characterized by an increase in both spontaneous and inducible production of free radicals. In FMF patients probability of transient activation of chemotaxis- and phagocytosis-dependent respiratory burst is higher. This has an important influence rather on production of free radical by activated cells than on their number.


Subject(s)
Familial Mediterranean Fever/immunology , Monocytes/immunology , Neutrophils/immunology , Respiratory Burst , Adolescent , Adult , Free Radicals , Humans , Neutrophil Activation , Phagocytosis
4.
Ter Arkh ; 77(12): 39-43, 2005.
Article in Russian | MEDLINE | ID: mdl-16514818

ABSTRACT

AIM: To study spontaneous chemotaxis-, phagocytosis-, and proteinkinase C-mediated respiratory splash (RS) of neutrophils and monocytes in colchicin-untreated patients with familial Mediterranean fever (FMF). MATERIAL AND METHODS: Of 17 FMF patients, 8 ones were examined during the attack, 9 patients--in fever-free period. Spontaneous and induced RS of peripheral blood neutrophils and monocytes was investigated with quantitative flow-cytofluorimetric method. RESULTS: Compared to healthy donors, RS is characterized with activation of both spontaneous and induced production of free radicals. The activity and intensity of the RS in FMF was low in the attack vs in the attack-free period but monocytes population has a stable high activity of the RS. CONCLUSION: Activation of neutrophilic RS in FMF patients is characterized by periodicity the direction of which is opposite to induced monocyte activation in the attack and in attack-free interval.


Subject(s)
Familial Mediterranean Fever/blood , Monocytes/metabolism , Neutrophils/metabolism , Reactive Oxygen Species/blood , Respiratory Burst/physiology , Adolescent , Adult , Biomarkers/blood , Flow Cytometry , Humans , Severity of Illness Index
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