Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 194
Filter
1.
Georgian Med News ; (348): 47-53, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38807390

ABSTRACT

Emotional intelligence (EI) is an important psychological aspect that has a significant impact on the diagnosis and psychotherapy of mental disorders. It includes the ability to effectively recognise, understand, and regulate one's own emotions, as well as the ability to perceive and interact with the emotions of others. The purpose of the study was to assess and compare the role of emotional intelligence (EI) in different methods of diagnosing and treating mental disorders, as well as its impact on therapy outcomes. The study found that the development of EI improves therapy outcomes by increasing patients' emotional awareness and self-regulation. In addition, it is worth noting that minimising the likelihood of relapse in mental illness is associated with the ability of patients to cope with stress and overcome difficult circumstances. To sum up: In addition, developing emotional intelligence can improve patients' well-being by enhancing their interpersonal relationships, expanding their social network, and mitigating feelings of social isolation. The results of the study indicate that EI should be taken into account in clinical practice and that new psychotherapeutic techniques can be developed to improve the outcomes of the treatment of mental disorders.


Subject(s)
Emotional Intelligence , Mental Disorders , Psychotherapy , Humans , Mental Disorders/therapy , Mental Disorders/diagnosis , Mental Disorders/psychology , Psychotherapy/methods , Male , Adaptation, Psychological , Female , Emotions/physiology
2.
Zh Nevrol Psikhiatr Im S S Korsakova ; 123(3. Vyp. 2): 20-25, 2023.
Article in Russian | MEDLINE | ID: mdl-36950817

ABSTRACT

OBJECTIVE: To evaluate the predictors of the lethal outcome of hemorrhagic stroke in young people. MATERIAL AND METHODS: A retrospective analysis of cases of non-traumatic hemorrhagic stroke was carried out in 60 young patients hospitalized in the neurosurgical department in the Ufa Clinical Emergency Hospital. By gender, the male predominated. The mean age of the patients was 37.4±5.1 years. Lethal outcome was in 18 (30%) patients. RESULTS: Data analysis revealed a relationship between the lethal outcome of hemorrhagic stroke and certain clinical, somatic, laboratory and instrumental factors. As a result, a significant model (p<0.001) of the probability of a lethal outcome in young patients was developed. CONCLUSION: The identified predictors of the probability of death from stroke can serve as the basis for adequate management of young patients with hemorrhagic stroke.


Subject(s)
Hemorrhagic Stroke , Stroke , Humans , Male , Adolescent , Adult , Retrospective Studies , Risk Factors
3.
Zh Nevrol Psikhiatr Im S S Korsakova ; 122(3. Vyp. 2): 5-10, 2022.
Article in Russian | MEDLINE | ID: mdl-35318836

ABSTRACT

OBJECTIVE: Analysis of etiopathogenetic factors and features of the course of hemorrhagic stroke in young people. MATERIAL AND METHODS: A retrospective analysis of 402 case histories with non-traumatic hemorrhagic stroke was carried out, of which 60 (14.93%) patients were young. The average age was 37.37±5.09 years. There were 19 (31.7%) females and 41 (68.3%) males. Statistical data processing was carried out using Microsoft Office 2010 and SPSS v.26. RESULTS: The main causes of hemorrhagic stroke in young people were changes in cerebral vessels by the type of aneurysmal deformation of the vascular wall, including Moya-Moya disease, and arteriovenous malformations (60%). The second etiological factor of hemorrhagic stroke was arterial hypertension (40%). Intracerebral hematoma was the most common type of hemorrhagic stroke. The functional outcome was influenced by: the severity of the patient's general condition, the severity of the neurological deficit, the degree of consciousness, the presence of concomitant pathology, convulsive, dislocation syndrome, leukocytosis, hyperglycemia, high creatinine levels. CONCLUSION: The problem of hemorrhagic stroke at a young age is complex and relevant, since the etiological factor of the disease at this age differs significantly from that in older age groups, requires adequate diagnostic searches to identify the etiopathogenetic factor and subsequent secondary prevention.


Subject(s)
Hemorrhagic Stroke , Hypertension , Moyamoya Disease , Adolescent , Adult , Aged , Cerebral Hemorrhage/etiology , Female , Humans , Hypertension/complications , Male , Moyamoya Disease/complications , Retrospective Studies
4.
Angiol Sosud Khir ; 27(4): 34-40, 2021.
Article in Russian | MEDLINE | ID: mdl-35050247

ABSTRACT

AIM: The purpose of the study was to demonstrate the possibilities and efficacy of endovascular interventions in patients with visceral artery aneurysms. PATIENTS AND METHODS: From 2013 to June 2020, we performed 15 embolizations of visceral artery aneurysms in 15 patients (8 men and 7 women). The technical success rate was 100%. The patients' age varied from 32 to 71 years. In 8 cases, the aneurysm was located in the basin of the splenic artery, in 3 cases in the gastroduodenal artery, in 2 cases in the superior mesenteric artery and in 2 cases in the renal artery. Three aneurysms were classified as false, and 12 as true. All aneurysms were saccular, with their diameter ranging from 6 to 32 mm. In 2 patients aneurysms were detected after endured infective endocarditis, in 8 during examination in abdominal pain syndrome, and in 5 cases as an incidental finding while performing computed tomography of abdominal organs. One patient was subjected to embolization of the afferent vessel, two underwent implantation of stent grafts into the aneurysmal neck area, and eleven underwent aneurysmal sac embolization with microcoils. In 9 cases, embolization was performed using microcoils only in 1 case using microcoils with balloon assistance, and in 2 cases using microcoils with stent assistance. We used from 1 to 12 microcoils, with a diameter of from 2 to 30 mm. RESULTS: During endovascular embolization of visceral artery aneurysms there were no target-organ infarctions, haemorrhagic complications, nor lethal outcomes. In the early postoperative period, 7 patients developed pain syndrome. In 1 case pain syndrome was relieved by narcotic analgesics, in 4 cases by spasmolytics, and in 2 cases by a combination of spasmolytics and non-narcotic analgesics. CONCLUSION: Endovascular treatment of visceral arterial aneurysms is an efficient and safe method.


Subject(s)
Aneurysm, False , Aneurysm , Adult , Aged , Aneurysm/diagnosis , Aneurysm/surgery , Aneurysm, False/diagnosis , Aneurysm, False/etiology , Aneurysm, False/surgery , Female , Humans , Male , Middle Aged , Splenic Artery , Treatment Outcome , Viscera
5.
Angiol Sosud Khir ; 26(1): 138-142, 2020.
Article in Russian | MEDLINE | ID: mdl-32240149

ABSTRACT

Aneurysms and pseudoaneurysms of visceral arteries are relatively rare clinical entities and their pathogenesis has not yet been fully understood. Aneurysmal rupture appears to be a complication leading to life-threatening internal haemorrhage and is a cause of high mortality. However, advances in endovascular surgery techniques have extended the capabilities of treating aneurysms, allowing microemboli to be delivered even to the hard-to-reach portions of the vascular bed with complicated anatomy. Presented in the article is a clinical case report regarding embolization of a false aneurysm of the inferior gastrointestinal artery, separated by a microcoil, and demonstrating possibilities of endovascular superselective embolization of the target vessel.


Subject(s)
Aneurysm, False/diagnosis , Embolization, Therapeutic , Endovascular Procedures , Hepatic Artery , Humans , Treatment Outcome
6.
Kardiologiia ; 59(4): 92-96, 2019 Apr 18.
Article in Russian | MEDLINE | ID: mdl-31002046

ABSTRACT

The article presents a clinical case of embolism with bone cement of the right ventricle of the heart and pulmonary artery after percutaneous vertebroplasty in a patient aged 63 years. According to the results of a comprehensive examination using ultrasound and x-ray methods, three foreign bodies were found: in the right ventricle cavity, in the trunk of the pulmonary artery, in the branches of the left pulmonary artery. Considering the stable condition, normal blood oxygen saturation, the lack of influence of formations on intracardiac hemodynamics, it was decided to refrain from surgery, since the risk of intervention exceeded the possible benefit. Conservative treatment tactics and dynamic observation were chosen. The literature data on the frequency of such events and tactics of management of these patients are presented.


Subject(s)
Heart Diseases , Pulmonary Embolism , Vertebroplasty , Bone Cements , Humans , Middle Aged , Pulmonary Artery
7.
Khirurgiia (Mosk) ; (7): 4-7, 2014.
Article in Russian | MEDLINE | ID: mdl-25146534

ABSTRACT

Hybrid operations combining open and endovascular surgeries are used in cardio-vascular surgery for the last 10-15 years. It leads to decrease complications frequency and mortality in case of pronounced comorbidities and severe heart, aorta and its branches disease. Authors have experience in performing of 10 hybrid surgeries and 7 aneurysms endoprosthesis of abdominal aorta. All operated patients had severe comorbidities which significantly increase risk of open surgery. These comorbidities were contraindication for open surgery in patients with abdominal aorta aneurysm. Thanks to introduction into practice hybrid operations and aorta aneurysms endoprosthesis the authors decreased complications frequency and avoided deaths in operated patients.


Subject(s)
Aortic Aneurysm, Abdominal , Endovascular Procedures , Postoperative Complications/prevention & control , Aged , Aortic Aneurysm, Abdominal/epidemiology , Aortic Aneurysm, Abdominal/surgery , Comorbidity , Contraindications , Endovascular Procedures/adverse effects , Endovascular Procedures/methods , Female , Heart Diseases/epidemiology , Heart Diseases/physiopathology , Humans , Male , Middle Aged , Minimally Invasive Surgical Procedures/adverse effects , Minimally Invasive Surgical Procedures/methods , Outcome Assessment, Health Care , Risk Adjustment , Russia , Severity of Illness Index , Vascular Grafting
8.
Vestn Khir Im I I Grek ; 173(1): 58-61, 2014.
Article in Russian | MEDLINE | ID: mdl-25055513

ABSTRACT

The article presents an analysis of the features of primary peritonitis in children. Medical reports of 182 patients with primary and appendicular peritonitis were analyzed. It was significant, that the sick girls aged 4-7 years often had the primary peritonitis after acute inflammatory processes, which took place a month earlier. Chronic infection foci were noted in these patients. The age-specific features of maturation of the immune and reproductive systems predisposed the disease.


Subject(s)
Appendicitis/complications , Peritonitis , Surgical Procedures, Operative , Abdomen, Acute/diagnosis , Abdomen, Acute/epidemiology , Abdomen, Acute/surgery , Abdominal Cavity/physiopathology , Adolescent , Age Factors , Child , Child, Preschool , Female , Humans , Laparoscopy/methods , Laparoscopy/statistics & numerical data , Male , Peritonitis/diagnosis , Peritonitis/etiology , Peritonitis/physiopathology , Peritonitis/surgery , Preoperative Period , Retrospective Studies , Risk Assessment , Russia/epidemiology , Sex Factors , Surgical Procedures, Operative/methods , Surgical Procedures, Operative/statistics & numerical data
9.
Vestn Khir Im I I Grek ; 173(2): 57-60, 2014.
Article in Russian | MEDLINE | ID: mdl-25055536

ABSTRACT

An immunological research of the patients and control group with primary peritonitis was made. The main indices of immune response were in the range of age rates. However, the immune signs of foci of chronic inflammation were revealed in patients after operation. The consequences of disease (an abdominal cavity effusion) were eliminated during surgical interventions, but not the cause of the inflammation. Children with the primary peritonitis should be examined for detection of infection foci.


Subject(s)
Abdominal Cavity/surgery , Antibody Formation , Immunity, Cellular , Immunoglobulins/blood , Infections/complications , Peritonitis , Postoperative Complications , Abdominal Cavity/physiology , Abdominal Cavity/physiopathology , Adolescent , Child , Child, Preschool , Female , Humans , Infections/immunology , Monitoring, Immunologic/methods , Peritonitis/etiology , Peritonitis/immunology , Peritonitis/physiopathology , Peritonitis/surgery , Postoperative Complications/immunology , Postoperative Complications/prevention & control , Postoperative Period
10.
Eksp Klin Farmakol ; 76(6): 7-10, 2013.
Article in Russian | MEDLINE | ID: mdl-24003481

ABSTRACT

The effect of 5-hydroxyadamantane-2-one was studied on morphological state of brain tissue of rats after the occlusion of left middle cerebral artery. It was shown that intraperitoneal administration of 5-hydroxyadamantane-2-on at dose 100mg/kg, in 30 minutes after occlusion and once daily for the following 6 and 12 days leads to significant regenerative processes in brain tissue. These processes were more profound after the 12-day treatment with 5-hydroxyadamanta-ne-2-one.


Subject(s)
Adamantane/analogs & derivatives , Adamantane/pharmacology , Brain Ischemia/drug therapy , Brain/drug effects , GABA Agonists/pharmacology , Protective Agents/pharmacology , Animals , Animals, Outbred Strains , Brain/blood supply , Brain/physiopathology , Brain Ischemia/etiology , Brain Ischemia/physiopathology , Cerebrovascular Disorders/complications , Cerebrovascular Disorders/physiopathology , Drug Administration Schedule , Injections, Intraperitoneal , Male , Rats
11.
Neuroscience ; 253: 132-41, 2013 Dec 03.
Article in English | MEDLINE | ID: mdl-23994182

ABSTRACT

Although surgical trauma activates the anterior pituitary gland and elicits an increase in prolactin (PRL) serum levels that can modulate nociceptive responses, the role of PRL and the PRL-receptor (PRL-R) in thermal and mechanical hyperalgesia in postoperative pain is unknown. Acute postoperative pain condition was generated with the use of the hindpaw plantar incision model. Results showed endogenous PRL levels were significantly increased in serum, operated hindpaw and spinal cords of male and female rats 24h after incision. These alterations were especially pronounced in females. We then examined the role of the PRL system in thermal and mechanical hyperalgesia in male and female mice 3-168 h after plantar incision with the use of knock-out (KO) mice with PRL or PRL-R gene ablations and in wild-type (WT) mice. WT mice showed postoperative cold hyperalgesia in a sex-dependent manner (only in females), but with no effect on heat hyperalgesia or mechanical allodynia in either sex. Studies in KO mice showed no effect of PRL and PRL-R gene ablation on heat and cold hyperalgesia in male mice, while heat hyperlgesia were reduced 3-72 h post-surgery in female PRL and PRL-R KO mice. In contrast, PRL and PRL-R ablations significantly attenuated mechanical allodynia 3-72 h post-surgery in both male and female mice. Overall, we found elevated PRL levels in serum, hindpaws and spinal cords after incision, and identify a contributory role for the PRL system in postoperative pain responses to thermal stimuli in females and to mechanical stimuli in both males and females.


Subject(s)
Hyperalgesia/metabolism , Pain Threshold/physiology , Pain, Postoperative/complications , Prolactin/metabolism , Receptors, Prolactin/metabolism , Analysis of Variance , Animals , Disease Models, Animal , Female , Functional Laterality , Hyperalgesia/genetics , Hyperalgesia/pathology , Male , Mice , Mice, Knockout , Pain Measurement , Pain, Postoperative/genetics , Pain, Postoperative/pathology , Physical Stimulation/adverse effects , Prolactin/blood , Rats , Receptors, Prolactin/deficiency , Sex Factors , Spinal Cord/metabolism , Time Factors
12.
Biofizika ; 58(4): 690-6, 2013.
Article in Russian | MEDLINE | ID: mdl-24455889

ABSTRACT

Verhulst's logistic differential equation, popular in mathematical ecology, is used in modeling of population growth, neural networks, statistics, reaction models, Fermi distribution, modeling of tumor growth, etc. We used this function to characterize growth of commensal Escherichia coli isolates from gut microflora in Crohn's disease patients. The results of our investigations show differences in growth parameters of commensal E. coli, isolated from the gut microflora in Crohn's disease patients and healthy volunteers; it is most likely explained by the influence of chronic inflammatory processes on growth and reproduction of these bacteria. It has been established that the used mathematical model well characterizes growth of patients' gut E. coli isolates, and it can be important for the expedient probiotics' application during the disease.


Subject(s)
Crohn Disease/microbiology , Escherichia coli Infections/microbiology , Escherichia coli/growth & development , Models, Statistical , Case-Control Studies , Crohn Disease/complications , Culture Media , Escherichia coli/isolation & purification , Escherichia coli Infections/complications , Feces/microbiology , Humans , Male
13.
Angiol Sosud Khir ; 18(2): 143-6, 2012.
Article in Russian | MEDLINE | ID: mdl-22929685

ABSTRACT

The authors describe herein a case report concerning stagewise endovascular treatment of a 69-year-old male patient presenting with occlusion of the right and left internal carotid arteries admitted to Clinical Hospital № 1 of the RF President's Affairs Administration with the following diagnosis: atherosclerotic occlusion of carotid arteries, arterial hypertension, chronic bronchitis, chronic prostatitis, and a history of multiple episodes of unconsciousness. Duplex scanning and angiography of the brachiocephalic arteries revealed occlusion of the both internal carotid arteries, as well as pronounced narrowing of the ostium of the right and left vertebral arteries supplying blood to all portions of the brain. Computed tomography revealed no signs of endured stroke. The first stage consisted in stenting of critical stenosis of the left vertebral artery ostium, followed by stenting of the right vertebral artery ostium performed 2 weeks after (with implanting drug-covered stents «Promus¼ in both cases). The patient was discharged home in a satisfactory condition.


Subject(s)
Angioplasty/methods , Brain Ischemia/prevention & control , Carotid Stenosis , Vertebral Artery , Aged , Angiography , Brain Ischemia/etiology , Brain Ischemia/physiopathology , Carotid Artery, Internal/pathology , Carotid Artery, Internal/physiopathology , Carotid Stenosis/diagnosis , Carotid Stenosis/physiopathology , Collateral Circulation , Drug-Eluting Stents , Humans , Male , Tomography, X-Ray Computed , Treatment Outcome , Ultrasonography, Doppler, Duplex , Vertebral Artery/pathology , Vertebral Artery/physiopathology , Vertebral Artery/surgery
14.
J Dent Res ; 90(6): 759-64, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21393555

ABSTRACT

Recent studies have demonstrated that the lipopolysaccharide (LPS) receptor (TLR4) is expressed in TRPV1 containing trigeminal sensory neurons. In this study, we evaluated whether LPS activates trigeminal neurons, and sensitizes TRPV1 responses via TLR4. To test this novel hypothesis, we first demonstrated that LPS binds to receptors in trigeminal neurons using competitive binding. Second, we demonstrated that LPS evoked a concentration-dependent increase in intracellular calcium accumulation (Ca(2+))(i) and inward currents. Third, LPS significantly sensitized TRPV1 to capsaicin measured by (Ca(2+))(i), release of calcitonin gene-related peptide, and inward currents. Importantly, a selective TLR4 antagonist blocked these effects. Analysis of these data, collectively, demonstrates that LPS is capable of directly activating trigeminal neurons, and sensitizing TRPV1 via a TLR4-mediated mechanism. These findings are consistent with the hypothesis that trigeminal neurons are capable of detecting pathogenic bacterial components leading to sensitization of TRPV1, possibly contributing to the inflammatory pain often observed in bacterial infections.


Subject(s)
Lipopolysaccharides/metabolism , Nociceptors/metabolism , TRPV Cation Channels/metabolism , Toll-Like Receptor 4/metabolism , Trigeminal Ganglion/metabolism , Animals , Binding, Competitive , Calcitonin Gene-Related Peptide/biosynthesis , Calcium Signaling , Capsaicin/pharmacology , Cells, Cultured , Male , Nociceptors/drug effects , Patch-Clamp Techniques , Rats , Rats, Sprague-Dawley , Sensory System Agents/pharmacology , Toll-Like Receptor 4/agonists , Trigeminal Ganglion/cytology
15.
Vestn Oftalmol ; 127(6): 6-10, 2011.
Article in Russian | MEDLINE | ID: mdl-22442985

ABSTRACT

62 patients (109 eyes) with glaucoma-like optic nerve head cupping and normal IOP indices (Po not more than 21 mm Hg) were examined to find the etiology of optic nerve changes. Regarding corneal biomechanics new-onset primary open-angle glaucoma (POAG) and normal tension glaucoma (NTG) were diagnosed in 27% and 3% of examined patients respectively. In 59% of patients optic neuropathy (ON) of different etiology was found to be the cause of glaucoma-like optic nerve changes including compression ON due to lesion of intracranial portion of optic nerve and chiasma in 6% of cases. In 11% physiologic large optic nerve head cupping with normal visual functions and absence of nerve fiber structural changes was found.


Subject(s)
Low Tension Glaucoma/diagnosis , Optic Nerve Diseases/diagnosis , Adult , Aged , Diagnosis, Differential , Female , Humans , Low Tension Glaucoma/etiology , Low Tension Glaucoma/pathology , Optic Nerve Diseases/etiology , Optic Nerve Diseases/pathology
16.
Neuroscience ; 171(4): 1109-19, 2010 Dec 29.
Article in English | MEDLINE | ID: mdl-20884333

ABSTRACT

The transient receptor potential A1 (TRPA1) channel contributes to nociceptive signaling in certain pain models. It has been suggested that Ca(2+), which activates and modulates TRPA1, could play a critical regulatory role in this process. Since TRPA1 and transient receptor potential V1 (TRPV1) channels are co-expressed and interact in neurons, we investigated whether activation and modulation of TRPA1 by Ca(2+) is regulated by TRPV1. Cell-attached recordings showed that TRPA1 is activated by extracellular Ca(2+) ([Ca(2+)](e)) in concentration-response fashion. This activation, especially by 2 mM [Ca(2+)](e) was substantially suppressed by co-expression with TRPV1. Inside-out recordings demonstrated that intracellular Ca(2+) ([Ca(2+)](i))-triggered activation of TRPA1 was attenuated by the presence of TRPV1 only at 2 mM [Ca(2+)](e), but not in Ca(2+)-free conditions. Further, depletion of internal Ca(2+) stores by thapsigargin generated TRPA1-mediated currents, which is affected by TRPV1 in both Chinese hamster ovary cells and sensory neurons. Since mustard oil current (I(MO)) is modulated by [Ca(2+)](e), we next examined whether alterations in the Ca(2+)-permeability of TRPV1 by mutating Y671 effect I(MO) properties. First it was demonstrated that the mutations in TRPV1 did not affect association of the TRPA1 and TRPV1 channels. However, these TRPV1 mutations, particularly Y671K, altered the following characteristics of TRPA1: magnitude of I(MO) in presence and absence of [Ca(2+)](e); the influence of [Ca(2+)](e) on the voltage-dependency of I(MO), and open probability of single-channel I(MO). In summary, activation of TRPA1 by [Ca(2+)](e) and [Ca(2+)](i) is controlled by the TRPV1 channel, and characteristics of I(MO) depend on Ca(2+) permeability of the TRPV1 channel.


Subject(s)
Calcium/metabolism , Sensory Receptor Cells/physiology , TRPV Cation Channels/metabolism , Transient Receptor Potential Channels/metabolism , Analysis of Variance , Animals , Calcium/pharmacology , Capsaicin/pharmacology , Cells, Cultured , Cricetinae , Cricetulus , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Gene Expression Regulation/drug effects , Green Fluorescent Proteins/genetics , Immunoprecipitation/methods , Membrane Potentials/drug effects , Membrane Potentials/physiology , Mice , Mice, Transgenic , Mustard Plant , Mutation/genetics , Patch-Clamp Techniques/methods , Plant Oils/pharmacology , Rats , Rats, Sprague-Dawley , Sensory System Agents/pharmacology , TRPA1 Cation Channel , TRPV Cation Channels/genetics , Thapsigargin/pharmacology , Transfection/methods , Trigeminal Ganglion/cytology
17.
Angiol Sosud Khir ; 16(1): 133-6, 2010.
Article in Russian | MEDLINE | ID: mdl-20635729

ABSTRACT

Presented herein is a clinical case report concerning a 59-year-old male patient suffering from multifocal atherosclerosis after endured repeat acute ischaemic attacks in various arterial basins of the brain. Analysing his case history revealed functional class II-IV angina of effort. Ultrasonographic and angiographic findings showed a haemodynamically significant plaque in the ostium of the left internal carotid artery, hypoplasia of the right vertebral artery, a haemodynamically meaningful narrowing of the left vertebral artery, subtotal stenosis of the anterior interventricular branch and pronounced alterations in the right vertebral artery. The first stage of surgery consisted in stenting of the left internal carotid artery and anterior interventricular branch, followed by the second stage consisting in the stenting of the left vertebral artery with an angiographically good outcome. The check-up CT of the brain and 24-hour ECG monitoring showed no negative dynamics. In July 2005, the patient was subjected to craniocerebral microarterial shunting on the right. No relapses of AIAs have so far been observed.


Subject(s)
Angioplasty, Balloon , Atherosclerosis/therapy , Brain Ischemia/prevention & control , Carotid Artery, Internal , Carotid Stenosis/therapy , Coronary Vessels , Stents , Vertebral Artery , Angiography , Atherosclerosis/diagnosis , Atherosclerosis/diagnostic imaging , Carotid Artery, Internal/diagnostic imaging , Carotid Stenosis/diagnosis , Carotid Stenosis/diagnostic imaging , Electrocardiography , Electrocardiography, Ambulatory , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Monitoring, Ambulatory , Tomography, X-Ray Computed , Treatment Outcome , Vertebral Artery/diagnostic imaging
18.
Neuroscience ; 155(2): 503-9, 2008 Aug 13.
Article in English | MEDLINE | ID: mdl-18582539

ABSTRACT

Tumor necrosis factor alpha (TNFalpha), a pro-inflammatory cytokine, enhances the development of pain and hyperalgesia, although the molecular mechanisms are not well understood. This study evaluated the hypothesis that TNFalpha increases the sensitivity of rat trigeminal neurons to capsaicin via two different mechanisms triggered by either brief or sustained exposure to the cytokine. A brief (5 min) application of TNFalpha significantly sensitized capsaicin-evoked accumulation of intracellular calcium ([Ca2+]i) (226.4+/-37.7 nM vs. 167.5+/-31.3 nM) and increased capsaicin-evoked nocifensive behavior (78.3+/-9.7 vs. 30.9+/-3.6 s) as compared with vehicle pretreatment (P<0.01 for both). Sustained (30 min to 4 h) exposure of cultured neurons to TNFalpha evoked a twofold increase in mRNA transcript (P<0.05) and protein levels (P<0.01) of transient potential receptor vanilloid type 1 (TRPV1). This long-term up-regulation of TRPV1 expression by TNFalpha correlated with enhancement in capsaicin-induced calcitonin gene-related peptide release (P<0.05). Demonstration of colocalization of TNFalpha receptor subtypes I and II with TRPV1 in almost all (>90%) TRPV1 expressing neurons provides evidence consistent with a direct interaction on the same subpopulation of sensory neurons. In summary, our data demonstrate that TNFalpha directly enhances the sensitivity of rat trigeminal neurons to capsaicin via both rapid, non-genomic mechanisms as well as sustained genomic regulation in TRPV1 expression. Thus, increased sensitization and up-regulation of TRPV1 constitutes a potential mechanism by which TNFalpha mediates inflammatory hyperalgesia and pain.


Subject(s)
Capsaicin/pharmacology , Neurons/drug effects , Trigeminal Nerve/cytology , Trigeminal Neuralgia/physiopathology , Tumor Necrosis Factor-alpha/pharmacology , Animals , Calcitonin Gene-Related Peptide/metabolism , Calcium/metabolism , Cells, Cultured , Drug Synergism , Gene Expression/drug effects , Male , Neurons/cytology , Neurons/physiology , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Receptors, Tumor Necrosis Factor, Type I/genetics , Receptors, Tumor Necrosis Factor, Type II/genetics , Sensory System Agents/pharmacology , TRPV Cation Channels/genetics , TRPV Cation Channels/metabolism , Time , Up-Regulation/drug effects
19.
J Dent Res ; 86(6): 550-5, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17525356

ABSTRACT

The transient receptor potential ankyrin repeat 1 (TRPA1) channel is believed to be involved in many forms of acute and chronic hyperalgesia. Nerve Growth Factor (NGF) regulates chronic inflammatory hyperalgesia by controlling gene expression in sensory neurons, including genes involved in inflammatory hyperalgesia in the dental pulp. We hypothesized that NGF increases functional activities of the TRPA1 channel in trigeminal ganglion neurons. Here, we show that NGF induced a concentration- and time-dependent up-regulation of TRPA1 mRNA in trigeminal ganglia neurons, as detected by real-time RT-PCR and in situ hybridization. In addition, NGF evoked a time-dependent increase of mustard oil (MO)-evoked TRPA1 activation in trigeminal ganglia neurons. Collectively, these findings demonstrate that NGF participates in the functional up-regulation of TRPA1 in trigeminal ganglia neurons. These enhanced activities of TRPA1 could play an important role in the development of hyperalgesia following nerve injury and inflammation in the orofacial region.


Subject(s)
Ankyrin Repeat/drug effects , Calcium Channels/drug effects , Facial Pain/physiopathology , Nerve Growth Factor/pharmacology , Transient Receptor Potential Channels/drug effects , Up-Regulation/drug effects , Animals , Ankyrins , Cells, Cultured , Dose-Response Relationship, Drug , Gene Expression Regulation/drug effects , Hyperalgesia/physiopathology , Immunohistochemistry , In Situ Hybridization , Male , Neurons/drug effects , Patch-Clamp Techniques , RNA, Messenger/drug effects , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction , TRPA1 Cation Channel , TRPC Cation Channels , Time Factors , Trigeminal Ganglion/drug effects
20.
Neuroscience ; 138(1): 17-24, 2006.
Article in English | MEDLINE | ID: mdl-16359816

ABSTRACT

Ligand-gated ion channels (ionotropic receptors) link to the cortical cytoskeleton via specialized scaffold proteins and thereby to appropriate signal transduction pathways in the cell. We studied the role of filamentous actin in the regulation of Ca influx through glutamate receptor-activated channels in third-order neurons of salamander retina. Staining by Alexa-Fluor 488-phalloidin, to visualize polymerized actin, we show localization of filamentous actin in neurites, and the membrane surrounding the cell soma. With Ca(2+) imaging we found that in dissociated neurons, depolymerization of filamentous actin by latrunculin A, or cytochalasin D significantly reduced glutamate-induced intracellular Ca(2+) accumulation to 53+/-7% of control value. Jasplakinolide, a stabilizer of filamentous actin, by itself slightly increased the glutamate-induced Ca(2+) signal and completely attenuated the inhibitory effect when applied in combination with actin depolymerizing agents. These results indicate that in salamander retinal neurons the actin cytoskeleton regulates Ca(2+) influx through ionotropic glutamate receptor-activated channels, suggesting regulatory roles for filamentous actin in a number of Ca(2+)-dependent physiological and pathological processes.


Subject(s)
Actins/physiology , Ambystoma/metabolism , Calcium/metabolism , Cytoskeleton/physiology , Glutamic Acid/toxicity , Retina/metabolism , Retinal Ganglion Cells/metabolism , Actins/metabolism , Animals , Bridged Bicyclo Compounds, Heterocyclic/pharmacology , Cells, Cultured , Cytochalasin D/pharmacology , Data Interpretation, Statistical , Depsipeptides/pharmacology , In Vitro Techniques , Microscopy, Confocal , Receptors, Glutamate/drug effects , Receptors, Glutamate/metabolism , Retina/cytology , Retinal Ganglion Cells/drug effects , Thiazoles/pharmacology , Thiazolidines
SELECTION OF CITATIONS
SEARCH DETAIL
...