[Case performed: simultaneous surgery for left coronary ostial stenosis and gastric cancer].

This report describes a case in which a 68-year-old male underwent two operations simultaneously for left coronary ostial stenosis and gastric cancer. Successfully performed procedures were a single coronary artery grafting with the saphenous vein to the left anterior descending artery, and a subtotal gastrectomy using the Billroth II method. The postoperative course was uneventful and the patient was discharged from the hospital in good condition after 42 days. At present, one-year postoperative, the patient has been visiting the outpatient clinic in healthy condition.

[Remnant-like particles-cholesterol (RLP-C) assay and its clinical application to lipid tests in a postprandial as well as fasting state].

It was during the past few years that postprandial hyperlipidemia is believed to be more closely related to a risk for coronary artery disease. However, the high variability in the postprandial triglyceride (TG) levels could have made overlooked such diagnostic value in estimating an individual's risk for coronary artery disease. To minimize the impact of such variability, the remnant lipoproteins, TG-rich lipoproteins, might be measured in cholesterol using remnant-like particles (RLP-C) assay as a more reliable marker. In place of the previous cholesterol reagent (CHOD-Iodine) in RLP-C assay, the RLP-C assay was improved by employing a new cholesterol reagent (POD-EMSE). The correlation between these two assays was high (r = 0.978). The variations in RLP-C levels in healthy normolipidemic subjects was within 7.5mg/dl in a day. In oral-fat loading test, the variations of RLP-C levels in healthy subjects were also within 7.5mg/dl. However, patients with coronary artery disease showed much higher levels (over 7.5mg/dl) of RLP-C during the test. In conclusion the RLP-C assay can be used for the measurement of remnant lipoproteins in serum prepared from the fasting as well as postprandial states for predicting subjects who may be at risk of coronary artery disease.

[A case of early gastric carcinoma which disappeared with treatment of low dose 5-FU].

A 60-year-old male with early gastric carcinoma of Ha type, had received colectomy for his carcinoma in transverse colon. He was treated after operation with 5-Fluorouracil (5-FU) orally at 200 mg per day. One and a half month after operation at a total dose 8.4 g 5-FU, the patient was examined on his stomach by endoscope and barium enema. The polypoid tumor in the stomach was not recognized there. No lesion has been noticed in his stomach endoscopically in the following 1 year.

Flavone glycosides from Asplenium normale.

Eight flavone glycosides were isolated from fronds of Asplenium normale, its two varieties, var. boreale and var. shimurae, and related species, A. oligophlebium. Six of the glycosides were identified: apigenin 7-O-dirhamnoside and 7-O-glucosylrhamnoside, luteolin 7-O-dirhamnoside and 7-O-glucosylrhamnoside, genkwanin 4'-O-glucosylrhamnoside, and vicenin-2. The remaining two glycosides were tentatively characterized as genkwanin 4'-O-glycoside and 6,8-di-C-glycosylluteolin. The four taxa analysed in this survey all had distinctive flavonoid profiles.

A serum-free culture of the neurons in the septal, preoptic, and hypothalamic region. Effects of triiodothyronine and estradiol.

Serial modifications of Bottenstein and Sato' serum-free hormone-supplemented medium resulted in a new promising medium (1 : 1 mixture of L15 and MCDB 104 containing several supplements) for culturing neonatal rat brain cells. This medium favored the morphological and biochemical differentiation of the neurons, including particular types of cholinergic and cholinoceptive neurons, obtained enzymatically from the septum, preoptic area, and hypothalamus. On the other hand, the growth of non-neuronal cells was markedly suppressed in this medium. Therefore, their effects on the neurons are minimized in this culture. Effects of triiodothyronine (T3) and estradiol (E2) on the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE), synthetic and degradative enzymes for acetylcholine, respectively, were examined in this culture. The optimal concentrations of T3 and E2 for AChE activity were around 1 nM and 10 pM, respectively. However, E2 appeared to be somewhat inhibitory at higher concentrations. Although the activity of ChAT was maximum around 10 pM of E2, the ChAT activity increased as the concentration of T3 was increased to 100 nM.