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Viruses ; 8(5)2016 05 21.
Article in English | MEDLINE | ID: mdl-27213433

ABSTRACT

Vaccinia virus (VACV) is one of the most extensively-studied viruses of the Poxviridae family. It is easy to genetically modify, so it has become a key tool for many applications. In this context, reporter genes facilitate the study of the role of foreign genes introduced into the genome of VACV. In this review, we describe the type of reporter genes that have been used to generate reporter-expressing VACV and the applications of the recombinant viruses obtained. Reporter-expressing VACV are currently employed in basic and immunology research, in the development of vaccines and cancer treatment.


Subject(s)
Genes, Reporter , Genetic Vectors , Molecular Biology/methods , Vaccinia virus/genetics , Virology/methods
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