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1.
Life Sci Alliance ; 5(2)2022 02.
Article in English | MEDLINE | ID: mdl-34848435

ABSTRACT

The nuclear RNA exosome plays a key role in controlling the levels of multiple protein-coding and non-coding RNAs. Recruitment of the exosome to specific RNA substrates is mediated by RNA-binding co-factors. The transient interaction between co-factors and the exosome as well as the rapid decay of RNA substrates make identification of exosome co-factors challenging. Here, we use comparative poly(A)+ RNA interactome capture in fission yeast expressing three different mutants of the exosome to identify proteins that interact with poly(A)+ RNA in an exosome-dependent manner. Our analyses identify multiple RNA-binding proteins whose association with RNA is altered in exosome mutants, including the zinc-finger protein Mub1. Mub1 is required to maintain the levels of a subset of exosome RNA substrates including mRNAs encoding for stress-responsive proteins. Removal of the zinc-finger domain leads to loss of RNA suppression under non-stressed conditions, altered expression of heat shock genes in response to stress, and reduced growth at elevated temperature. These findings highlight the importance of exosome-dependent mRNA degradation in buffering gene expression networks to mediate cellular adaptation to stress.


Subject(s)
Exosome Multienzyme Ribonuclease Complex/metabolism , RNA, Messenger/genetics , RNA, Nuclear/genetics , RNA-Binding Proteins/metabolism , Schizosaccharomyces/genetics , Schizosaccharomyces/metabolism , Stress, Physiological , Gene Expression Regulation, Fungal , Gene-Environment Interaction , RNA, Messenger/metabolism , RNA, Nuclear/metabolism
2.
Mol Cell ; 78(2): 236-249.e7, 2020 04 16.
Article in English | MEDLINE | ID: mdl-32101700

ABSTRACT

The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanistically relevant features of pericentric heterochromatin compaction in mouse fibroblasts. We find that (1) HP1 has only a weak capacity to form liquid droplets in living cells; (2) the size, global accessibility, and compaction of heterochromatin foci are independent of HP1; (3) heterochromatin foci lack a separated liquid HP1 pool; and (4) heterochromatin compaction can toggle between two "digital" states depending on the presence of a strong transcriptional activator. These findings indicate that heterochromatin foci resemble collapsed polymer globules that are percolated with the same nucleoplasmic liquid as the surrounding euchromatin, which has implications for our understanding of chromatin compartmentalization and its functional consequences.


Subject(s)
Chromatin/genetics , Chromosomal Proteins, Non-Histone/genetics , Euchromatin/genetics , Heterochromatin/genetics , Animals , Chromobox Protein Homolog 5 , Fibroblasts , Mice
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