Preparation and characterization of titanium dioxide nanoparticles and in vitro investigation of their cytotoxicity and antibacterial activity against Staphylococcus aureus and Escherichia coli.

Titanium dioxide nanoparticles (TiO2 NPs) are photo-active metallic nanoparticles becoming promising agents in modern biomedical applications. Herein, a novel process for the synthesis of TiO2 NPs with high stability was developed by a sol-gel process and to investigate their cytotoxicity and antibacterial activity. Numerous experiments have been performed to confirm the morphologies, compositions, and physicochemical properties of prepared TiO2 NPs, such as field emission scanning electron microscopy, dynamic light scattering, Zeta potential, Fourier transform infrared spectroscopy and X-ray diffraction. MTT assay was applied to assess the cytotoxicity of the prepared nanoparticles. The results indicate that the synthesized nanoparticles' diameter is about 68 nm and contains the anatase phase, in the range of 2θ from 25 to 80 °C. The hydrodynamic radius of nanoparticles is about 140.4 nm, and the zeta potential of nanoparticles is about -44.6 mV. The MTT results have not shown any toxicity; the antibacterial inhibitory effect of TiO2 NPs at 200 mg/mL concentrations exhibited superior antibacterial activity at 15.9 ± 0.1, 14.0 ± 0.1 against Staphylococcus aureus and Escherichia coli, respectively. In conclusion, colloidal solutions with high stability were successfully synthesized, contributing to decreased dimensions and increased antibacterial properties.

Serological and Histopathological Investigation of Chlamydia abortus in Aborted Ewes in Wasit, Iraq.

Chlamydia abortus is one of the most important pathogens, which causes a marked economic loss in small ruminants, in particular sheep, worldwide. This study aimed to detect the prevalence of C. abortus in the sera of aborted ewes in Wasit province, Iraq, using the enzyme-linked immunosorbent assay (ELISA), followed by the investigation of the main histopathological alterations that occurred in some organs of the dead newborns. Out of 180 tested samples by ELISA, 32.22% of the evaluated animals showed positive reactions to IgG antibodies toward C. abortus. Concerning the titers of the infection of seropositive ewes, there were significant increases in values of moderate level of antibody titer (55.17%), compared to mild (32.76%) and severe (12.07%) levels of infection. History data showed a significant variation (P<0.05) in the existence of seropositive ewes with other field animals. However, significant increases (P<0.05) were reported in the seropositive ewes existed with goat (91.38%) and cattle (84.48%), while significant decreases (P<0.05) were observed in positive ewes found in donkeys (8.62%), horses (3.45%), and camels (0%). Regarding the histopathology results, the findings of the small intestine showed marked necrosis of intestinal villous, hyperplastic tissues, and necrosis in some mucosal glands; however, in the liver, there was small ductal proliferation with mild portal fibrosis, necrotic debris, and focal areas of hemorrhage in the parenchyma. In conclusion, the findings of this study represent the first Iraqi data concerning Chlamydial detection in aborted ewes; however, further studies in other regions and animal species are necessary to know the actual prevalence of organisms and initiate active measures for control and prevention.

Effects of nonsurgical periodontal therapy on C-reactive protein and serum lipids in Jordanian adults with advanced periodontitis.

BACKGROUND AND OBJECTIVE: Data on whether periodontal therapy affects serum CRP levels are inconclusive. The aim of this study was to determine if nonsurgical periodontal therapy has any effect on CRP and serum lipid levels in patients with advanced periodontitis. MATERIAL AND METHODS: Thirty-six systemically healthy patients, ≥ 40 years of age and with advanced periodontitis, were recruited for the study. Patients were randomized consecutively to one of two groups: the treatment group (n = 18) or the control group (n = 18). Treated subjects received nonsurgical periodontal therapy, which included oral hygiene instructions and subgingival scaling and root planing. Systemic levels of inflammatory markers [C-reactive protein (CRP) and the lipid profile] were measured at baseline and 3 mo after periodontal therapy. RESULTS: Nonsurgical periodontal therapy in the treatment group resulted in a significant reduction in the serum CRP level. The average CRP level decreased from 2.3 mg/dL at baseline to 1.8 mg/dL (p < 0.005) after 3 mo of periodontal therapy. The average reduction (95% confidence interval) in CRP was 0.498 (95% confidence interval = 0.265-0.731). In the treatment group, the reduction in CRP was significantly, linearly and directly correlated with the reduction in the plaque index, the gingival index and the percentage of sites with pocket depth ≥ 7 mm (Pearson correlation coefficient = 0.746, 0.425 and 0.621, respectively). Nonsurgical periodontal therapy had no effect on the lipid parameters. CONCLUSION: This study demonstrated that nonsurgical periodontal therapy results in a significant reduction in the serum CRP level. The effect of this outcome on systemic disease is still unknown.