ABSTRACT
The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. At therapeutic doses of metronidazole (4.98-24.86 microg/mL) significant inhibition of the production of reactive oxygen species was noted in both methods. The inhibitory effect was in a dose-dependent pattern. The data suggest a scavenging mechanism of metronidazole on reactive oxygen species generated by PMN.
Subject(s)
Anti-Infective Agents/pharmacology , Free Radical Scavengers/pharmacology , Metronidazole/pharmacology , Neutrophils , Radiation-Sensitizing Agents/pharmacology , Reactive Oxygen Species/antagonists & inhibitors , Adult , Aerobiosis/drug effects , Aerobiosis/physiology , Anti-Infective Agents/chemistry , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Humans , Indicators and Reagents , Luminescent Measurements , Luminol , Metronidazole/chemistry , Middle Aged , NADP/drug effects , NADP/physiology , Neutrophils/drug effects , Neutrophils/physiology , Nitroblue Tetrazolium , Oxidative Stress/drug effects , Oxidative Stress/physiology , Phagocytosis/drug effects , Phagocytosis/physiology , Radiation-Sensitizing Agents/chemistry , Reactive Oxygen Species/analysisABSTRACT
The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear [PMN] cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. At therapeutic doses of metronidazole [4.98-24.86 microg/mL] significant inhibition of the production of reactive oxygen species was noted in both methods. The inhibitory effect was in a dose-dependent pattern. The data suggest a scavenging mechanism of metronidazole on reactive oxygen species generated by PMN
Subject(s)
Granulocytes , Radiation-Sensitizing Agents , Reactive Oxygen Species , MetronidazoleABSTRACT
HgCl2, added in vitro to human granulocytes in whole blood, caused a marked inhibitory effect on the luminol-dependent chemiluminescence induced by BaS04 crystals in suspension of these cells. The effect was both dose- and time-dependent when BaS04 was used to stimulate the oxidative burst in granulocytes. Incubation with the highest concentration of HgCl2 used [10 mmol/L], however, did not cause disruption of the membranes of granulocytes. The effect of HgCl2 on the granulocytes was irreversible following washing of the HgCl2-treated cells with phosphate buffered saline. HgCl2 did not affect chemiluminescence produced when luminol was excited by oxidative hydrogen peroxide in a cell-free medium. These results suggest that some of the toxicity of HgCl2 may be greater than mediated by an action on the phagocytic immune system
