Changes in the hematobiochemical, acid-base and blood gas elements as well as biomarkers of inflammation and bone metabolism in donkeys (Equus asinus) with acute bleeding.

Background: Acute hemorrhage is fatal in equines with a complication of severe hypovolemic shock that causes a sudden death in such cases. Aim: This study was designed to report the influences of acute bleeding in conscious non-sedated donkeys (Equus asinus) on the hematobiochemical variables, acid-base, blood gas elements, and markers of inflammation and bone metabolism. Methods: Eight healthy donkeys were used where a total of 900 ml of whole blood was collected. Five blood samples were collected from each animal: just before collection of blood (T0); (2) 30 (T1), 60 (T2), 120 (T3), and 240 minutes (T4) later. The blood panels including total white blood cells, lymphocytes, neutrophils, red blood cell counts (RBCs), HCT, hemoglobin (Hg), and RBCs indices were measured. Biochemical parameters and electrolytes were evaluated. The activity of aspartate aminotransferase (AST), creatine kinase (CK), and γ-glutamyl transferase (GGT) were also determined. Complete acid-base and blood gas panels were assessed. Serum amyloid A (SAA), haptoglobin (Hp), osteocalcin (OC), bone alkaline phosphatase (b-ALP), and pyridinoline cross-links (PYD) were measured. Results: The RBCs, Hg, and HCT increased significantly at points T1, T2, and T3 compared to T0. The concentrations of total proteins and albumin decreased significantly at points T3 and T4. The blood urea nitrogen concentrations increased significantly at T4. Creatinine concentrations increased significantly at T2 and T3. The AST, GGT, and CK decreased significantly. On the other hand, glucose increased significantly at T3 and T4. The pH decreased significantly at points T1, T2, T3, and T4. The PCO2 increased significantly at T3 and T4. The BE, HCO3, and TCO2 values decreased significantly at T2, T3, and T4. Contrary, the AG increased significantly at points T3 and T4. The potassium increased significantly at T1-T4 and chloride decreased significantly at T3 and T4. Lactate showed significant increases at T1-T4. The SAA, Hp, OC, b-ALP, and PYD did not differ significantly at T1-T4. Conclusion: In conscious non-sedated donkeys, induced bleeding resulted in significant changes in the hematobiochemical elements, the acid-base status, and blood gas and electrolyte parameters. However, it did not change the markers of inflammation and bone metabolism.

Intra-articular injection in the hind limb joints of dromedary camels (Camelus dromedarius) using anatomical and arthrographic-guided landmarks.

BACKGROUND AND AIM: A healthy joint is an important structure for the proper movement of the camel limb. Intra-articular (IA) injection is frequently used in veterinary practice for diagnostic and therapeutic purposes of joint injuries. Thus, the current study aimed to describe the injection of the hindlimb joints in dromedary camels based on the anatomical and arthrographic-guided landmarks. MATERIALS AND METHODS: Eighteen orthopedically sound adult camels (mean±standard deviation age: 78±12 months) of both sexes were included in this study. Three camels were euthanized to identify anatomical features in the hindlimb joints and related structures. IA injections were performed in the hindlimbs of 5 camel cadavers to evaluate the optimal IA injection site, which was confirmed by arthrography. The optimized IA injection technique was applied in 10 live camels and confirmed by arthrocentesis and arthrography. For each joint, injection criteria (number of attempts, difficulty of injection, and successful injection) were assessed, scored, and statistically compared to the other joints. RESULTS: The summation of IA injection criteria scores was significantly higher (p<0.05) in the femorotibial, femoropatellar, tibiotarsal, fetlock, pastern, and coffin joints in comparison to the hip joint. CONCLUSION: Anatomical and arthrographic-guided techniques offer considerable advantages for the characterization of anatomical landmarks and selection of the appropriate IA injection site in the hindlimb in dromedary camels. Furthermore, a reference approach for camels was established that is different from the approach for cattle and horses.

The Effects of 7000 Meter Race on Hematological and Biochemical Parameters in Racing Greyhounds.

This study was carried out to investigate the effects of 7000 meter race on hematological and biochemical profiles in racing greyhounds. Thirty-two male racing greyhounds were enrolled in this study. Blood samples were collected from the greyhounds at rest 24 hours prior to the race (T0), within 2 hours of the completion of the race (T1) and 24 hours after the race (T2). None of the hematological parameters differed significantly among T0, T1 and T2 time points. The serum activity of aspartate aminotransferase increased significantly after race compared to values before race. However, the serum activity of aspartate aminotransferase dropped 24 hours after race to a value comparatively similar to that before race. The serum activity of creatine kinase increased also sharply after race compared to values before race. The serum activity of creatine kinase dramatically dropped 24 hours after race to a value of comparable to values at T0. In a similar pattern, the serum activity of alkaline phosphatase increased significantly after race compared to values before race. The serum activity of alkaline phosphatase decreased 24 hours after race. The serum concentration of lactate increased sharply after race compared to values before race and dropped 24 hours after race to a mean value comparatively similar to that before race. Other biochemical variables that included total protein, albumin, globulin, blood urea nitrogen, calcium, phosphorus and magnesium did not differ significantly among T0, T1 and T2 values. It can be concluded that racing 7 km distance in greyhound dogs did not significantly change the hematological parameters but significantly increases the serum activity of musculoskeletal enzymes and concentration of lactate. The full recovery occurred 24 hours after the race, as the musculoskeletal enzymes and lactate level returned to the normal limits.

Can bone marrow aspirate improve mandibular fracture repair in camels (Camelus dromedarius)? A preliminary study.

BACKGROUND: Mandibular fractures are common in camels, leading to considerable economic losses. This study explored methods of improving mandibular fractures repair, adjuvant with interdental wire, or bone plate fixation. Autologous bone marrow (BM) injection enhances osteogenesis and rapid healing. OBJECTIVES: To investigate the effect of autologous BM aspirate as an adjuvant treatment for repairing mandibular fractures in camels with interdental wire, or bone plate fixation. METHODS: Thirty dromedary camels aged 5-8 years and of both sexes were randomly divided into 4 treatment groups: group 1 (n = 10) treated with stainless steel wire fixation and BM injection at the fracture line, group 2 (n = 10) treated with plate fixation and BM injection at the fracture line, group 3 (n = 5) treated with stainless steel bone wire fixation and placebo saline injection at the fracture line, and group 4 (n = 5) treated with plate fixation and placebo injection at the fracture line. The mandibular fractures were followed weekly for 12 weeks postoperatively to assess improvement and healing based on clinical evaluation, radiographic union scale, and bone turnover markers (i.e., bone alkaline phosphatase, osteocalcin, pyridinoline, and deoxypyridinoline). RESULTS: Compared to other groups, elevated bone turnover markers in group 1 were demonstrated (p < 0.05) on the seventh postoperative day. Likewise, compared to other groups, both clinical findings and radiographic union scale significantly improved (p < 0.05) in group 1 on the 56th postoperative day. CONCLUSIONS: BM aspirate has a promising beneficial osteogenic effect on mandibular fracture repair in camels, most notably when combined with interdental wire fixation.

Diagnosis and treatment of foreign bodies swallowing syndrome in camels (Camelus dromedarius) with special reference to the role of mineral deficiency.

This study describes the clinical presentation of ruminal and reticular foreign body syndrome (RRFBS), and evaluates the effect of mineral deficiency on its occurrence in dromedary camels. Thirty dromedary camels were divided into two groups. Group 1 (control) included 10 apparently healthy she-camels. Group 2 consisted of twenty dromedary camels diagnosed with RRFBS on the basis of clinical, ultrasonographic, hematological, and biochemical examinations. Clinical findings showed decreased appetite and milk yield, tympany, and gradual body weight loss. Ultrasonographic examinations revealed the presence of hyperechoic material with variable degrees of shadowing. Hematological evaluation showed a significant (P<0.05) decrease of the total erythrocyte and lymphocyte count and a significant increase of neutrophils in the camels with RRFBS compared to the controls. Biochemical tests showed a significant elevation in the activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatine kinase (CK), glucose, creatinine, and blood urea nitrogen and a significant decrease of sodium, chloride, potassium, cobalt, iron, and selenium in the camels with RRFBS compared to the controls. Rumenotomy was performed on the 20 camels as a surgical intervention for treating the RRFBS. By the 6th month postoperatively, all surgically treated camels had completely recovered except for one with tympany and slight swelling in situ. In conclusion, trace element deficiency might play an important role in the occurrence of foreign body ingestion syndrome in dromedary camels. Moreover, clinical, ultrasonographic, hematological, and biochemical examinations are considered as tools assisting in the accurate diagnosis, prognosis, and treatment stratagem for RRFBS in camels.

Diagnostic evaluation of subclinical endometritis in dromedary camels.

The objective of the present study was to evaluate the efficacy of various diagnostic methods and to estimate the prevalence of bacterial pathogens associated with subclinical endometritis (SCE) in dromedary camels. During two consecutive breeding seasons, a total of 2122 infertile female dromedaries were assigned to this study and suspected cases of SCE were identified using the established criteria which included failure to conceive after three or more consecutive matings with a fertile male, a clinically healthy genital system, no observable vaginal discharge, and normal sexual behavior. Manual vaginal examination, Metricheck, bacteriological examination using endometrial swabbing, and hemogram assessments were conducted and there were comparisons of results to when there was cytological examination using the Cytobrush technique as the gold standard. The threshold value for positive cases of SCE was set at ≥ 5% polymorphnuclear cells in the cytological samples. Subclinical endometritis was diagnosed in 211 9.94 %) of the total infertility cases. Endometrial swabbing was a more sensitive and specific technique for diagnoses compared with the other methods. Bacillus sp., Staphylococcus sp., and Candida albicans were the most commonly isolated microorganisms. Hemogram testing and rectal and ultrasonographic examinations were not effective for the diagnosis of SCE. It was concluded that, compared with other diagnostic tests, bacteriological examination is more sensitive and specific for the detection of SCE in dromedaries.

Correction to: Factors affecting reproductive performance in dromedary camel herds in Saudi Arabia.

This article was originally published with incorrect affiliation 1. The correction affiliation is presented below.

Scrotal enlargement in rams and bucks in Qassim region, central of Saudi Arabia: clinical and ultrasonographic findings and seroprevalence of brucellosis.

The aim of this study was to clarify the causes of scrotal enlargement in rams and bucks in Qassim region of Saudi Arabia. Enlarged scrotal contents of rams and bucks (n = 153) were examined by visual inspection, palpation, and ultrasonography. Blood samples were obtained and tested for Brucella sp. infection. Clinical and ultrasonographic findings showed that scrotal enlargement was mainly associated with orchitis, peri-orchitis, and epididymitis. Miscellaneous findings were scrotal hernia, scrotal hematoma, and hydrocele. The frequencies of orchitis, peri-orchitis, and epididymitis were 47.4, 21.1, and 14.1% in Awassi rams; 54.5, 21.7, and 8.7% in Najdi rams; 52.3, 20.5, and 9.1% in Ardi bucks; and 50, 16.7, and 16.7% in Damascus bucks, respectively. Orchitis was associated with no-abscess formation (23%), single-abscess formation (15.4%), and multiple-abscesses formation (61.6%). Peri-orchitis was characterized by hard consistency, atrophy of the testes, and extensive connective tissue formation. Epididymitis was observed mainly at the tail of the epididymis (82.4%) but rarely at the head (17.6%). Epididymitis was associated in many cases with abscessation (70.6%). Males with orchitis, peri-orchitis, and epididymitis were positive for Brucella melitensis and Brucella ovis in the frequency of 21.3% and 48.8%, respectively. In conclusion, scrotal enlargement in rams and bucks in Qassim region is caused mainly by inflammation of the testis and/or epididymis and associated tremendously with brucellosis seropositivity.

Azoospermia in male dromedary: Clinical findings, testicular biopsy, serum follicle stimulating hormone and seminal biomarkers.

Azoospermia is not an uncommon infertility problem in the male dromedary (Camelus dromedarius). Azoospermia was investigated via clinical findings, testicular biopsy as well as the evaluation of follicle stimulating hormone (FSH), concentration of camel testis protein (TEX101) and camel epididymis-specific extracellular matrix protein (ECM1) in seminal fluids. Azoospermic male camels (AZOO group, n = 28) that had been detected to be infertile as a result of lack of resulting pregnancies after repeated mating's for at least one season were included in this study. Clinical examination, semen analysis and testicular biopsy sampling were conducted for each individual animal. Blood samples were collected from the AZOO and from reference fertile males (FERT group, n = 8) for the assay of FSH hormone and semen biomarkers (TEX101 and ECM1). There were bilaterally normal-sized testes in 42.8%, bilaterally small-sized testes in 35.7%, bilaterally large-sized testes in 7.1%, no testicles in 7.1% and only one testicle in 7.1% of azoospermic animals. Sertoli cell-only syndrome (SCO) and maturation arrest were observed in 78.6% and 21.4% of the animals, respectively. There were greater concentrations of FSH in the AZOO group compared with the FERT group (P = 0.01). In conclusion, azoospermia in dromedary camels is mainly associated with spermatogenic defects and greater serum FSH concentrations. Seminal biomarkers, therefore, might be feasible indicators for identifying azoospermia in the male dromedary camels and the condition of non-obstructive azoospermia was seemingly prevalent in the male dromedary camels in the present study.

Impotentia generandi in male dromedary camels: FSH, LH and testosterone profiles and their association with clinical findings and semen analysis data.

Impotentia generandi (IG) is a major problem in male dromedary camels. The objective of this research was to characterize the FSH, LH and testosterone profiles and their association with clinical findings and semen characteristics in IG-male dromedaries. Semen was collected by electroejaculation from camels with IG (n = 17) and from a fertile group (FERT, n = 5) and was evaluated for motility, viability, abnormality and concentration. According to their sperm counts, the IG-camels were categorized into three groups: those with normal sperm concentration (IG-NC, n = 8), those with oligospermia (IG-OLIGO, n = 5) and those with azoospermia (IG-AZOO, n = 4). Jugular blood was collected from all camels for analysis of FSH, LH and testosterone (T) concentrations. Results showed that the FSH, LH and T levels were higher in the IG-OLIGO and IG-AZOO groups than in the FERT and IG-NC groups (P < 0.05). The IG-camels with small testes showed lower sperm counts (P < 0.01) and greater FSH and T levels (P < 0.05) than the IG-camels with normal-sized testes. Inverse relationships were observed between the sperm counts and both the FSH and the LH levels (P < 0.05). In conclusion, the IG dromedary camels with oligo- and azoospermia were characterized by high serum levels of FSH, LH and T, indicating a condition of spermatogenic failure. In addition, inverse correlations were observed between these hormones and testicular size and sperm count.

Factors affecting reproductive performance in dromedary camel herds in Saudi Arabia.

A survey of 7122 dromedary camels in 115 herds in Saudi Arabia was used to estimate the effects of herd size (HZ; < 25 vs. 25-49 vs. 50-100 vs. > 100 camels), herder/camels ratio (H/C; 1:< 25 vs. 1:25-50 vs. 1:> 50), manager experience (ME; < 5 vs. 5-10 vs. > 10 years), male/females ratio (M/F), housing system (HS; free vs. closed vs. mixed), length of the breeding season (winter vs. winter and spring vs. fall, winter and spring), age at first mating (3 vs. > 3 years), and time of mating after parturition (≤ 3 vs. > 3 months) and their interactions on the overall pregnancy rate. Barren females of these herds (n = 886) were examined for the causes of infertility. Results showed that herds with H/C of 1:< 25 had higher overall pregnancy rate (95.29%) than herds with H/C of 1:25-50 (79.84%) and those with H/C of 1:> 50 (72.79%) (p = 0.003). Herds having ME of > 10 years revealed greater overall pregnancy rate (94.89%) than herds with ME of 5-10 years (80.54%) and those with ME of < 5 years (72.5%) (p = 0.001). There were significant interactions between H/C × HZ (p = 0.003), H/C × HS (p = 0.006), and ME × HS (p = 0.02). The overall pregnancy rate did not significantly differ between herds bred females by age of 3 years and those bred females by age > 3 years and in females bred within 3 months after parturition and in those bred after 3 months. The mean calving interval was shorter (p = 0.008) in camels mated within 3 months of parturition (15.25 ± 2.8 months) than in those mated after that time (24.33 ± 6.5 months). Clinical endometritis, ovarian hydrobursitis, and vaginal adhesions were the common clinical findings in barren females. Thus, efforts to reduce the age at first mating and the interval after calving, increase the number of herders/camels, and control reproductive disorders could improve the reproductive performance and quality of camel herds in Saudi Arabia.

Ultrasonographic findings in goats with contagious caprine pleuropneumonia caused by Mycoplasma capricolum subsp. capripneumoniae.

BACKGROUND: In goats, contagious caprine pleuropneumonia (CCPP) is a cause of major economic losses in Africa, Asia and in the Middle East. There is no information emphasising the importance of diagnostic ultrasound in goats with CCPP caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp). This study was designed to describe the ultrasonographic findings in goats with CCPP caused by Mccp and to correlate ultrasonographic with post-mortem findings. To this end, 55 goats with CCPP were examined. Twenty-five healthy adult goats were used as a control group. RESULTS: Major clinical findings included harried, painful respiration, dyspnoea and mouth breathing. On ultrasonography, a liver-like echotexture was imaged in 13 goats. Upon post-mortem examination, all 13 goats exhibited unilateral pulmonary consolidation. Seven goats had a unilateral hypoechoic pleural effusion. At necropsy, the related lung was consolidated and the pleural fluid appeared turbid and greenish. Pleural abscessiation detected in five goats was confirmed post-mortem. Twenty-eight goats had a bright, fibrinous matrix extending over the chest wall containing numerous anechoic fluid pockets with medial displacement and compression of lung tissue. Echogenic tags imaged floating in the fluid were found upon post-mortem examination to be fibrin. In two goats, a consolidated right parenchyma was imaged together with hypoechoic pericardial effusions with echogenic tags covering the epicardium. At necropsy, the right lung was consolidated in three goats and fibrin threads were found covering the epicardium and pericardium. CONCLUSIONS: In goats with CCPP, the extension and the severity of the pulmonary changes could not be verified with clinical certainty in most cases, whereas this was possible most of the time with sonography, thus making the prognosis easier. Ultrasonographic examination of the pleurae and the lungs helped in the detection of various lesions.

Echography of clinically relevant disorders in the genital tract of female dromedary camels.

The aim of this study was to characterize the clinically relevant genital tract disorders of dromedary camels. Reproductive tract examinations were performed via transrectal palpation, ultrasonography and vaginal exploration. The ultrasonic appearance of the reproductive pathology was described and compared with its morphology at laparotomy, after surgical removal, during postmortem examination or upon slaughter. Diagnosis was also confirmed by histopathology. The most frequently encountered follicular structures were larger than typical follicles (56/338, 16.6%) having three echo textures: 1) thin walls and clear hyperechogenic content (11.6%); 2) thick walls and few fibrous trabeculae (33.7%); and 3) thick walls and many echogenic transecting fibrinous strands (54.7%). Corpora lutea with non-echoic central cavity (5/31, 16.1%) were greater in diameter than those with no cavity (26/31, 83.9%) (P=0.03). A granulosa cell tumor (1/338, 0.3%) was multilocular and honeycombed in shape. Presence of a large, well-demarcated, hypoechogenic sac lateral to or beneath the uterine horn encasing the ovary was diagnostic for ovarian hydrobursitis (102/338, 30.2%). Hydrosalpinx and pyosalpinx (6/338, 1.8%) were beaded in appearance, with the ovary located outside these structures. Clinical endometritis/cervicitis (122, 36.1%) was characterized by changes in the homogeneity in about half of the cases. A greatly dilated uterus with clear, hypoechogenic or echogenic contents with signs of hydrometra and pyometra, respectively, was another categorization of a reproductive pathology (24/338, 7.1%). Highly reflective, linear structures were observed in cases with intrauterine fetal bone retention (1/338, 0.3%). In conclusion, reproductive pathologies in dromedary camels can be efficiently imaged by use of ultrasonic technologies, thus familiarizing the practitioner with these disorders and facilitating application of these technologies so that suitable treatment can occur is important in managing reproduction of dromedary camels.

Erectile dysfunction in male dromedary camels: Clinical findings and changes in the nitric oxide metabolite, cardiac troponin I and testosterone concentrations.

This study aimed to clarify the phenomenon of erectile dysfunction (ED) in male camels and monitor the associated changes in nitric oxide metabolite (NOM), cardiac troponin I (cTnI), and testosterone concentrations. A total 18 camels with ED and 10 controls were included in this study. The breeding history was recorded and a thorough breeding soundness examination was performed. Total nitrates and nitrites were determined in sera using the Griess assay. Serum cTnI and testosterone were assessed using ELISA. A complete blood count was also carried out. The results showed that 13/18 male camels with ED had no detectable pathologic lesions in the genital tract (ED-N), while 5/18 males showed pathology in the penis, prepuce, and testicles (ED-P). The ED-P group exhibited higher concentrations of NOMs (P = 0.003), white blood cells (P = 0.0001), and neutrophils (P = 0.001) than the ED-N and control groups. The ED-P and ED-N groups had higher concentrations of cTnI than the control group (P = 0.0001). Testosterone concentration did not differ among the groups. In conclusion, the ED in the camels was associated with a rise in cTnI, probably due to myositic damage. Most of the ED cases in the camels had apparently normal genital organs. In the cases of ED in male camels with detectable pathologic lesions on their genital organs, a rise in NOMs, white blood cells, and neutrophils was observed.

Influence of the cardiac glycoside digoxin on cardiac troponin I, acid-base and electrolyte balance, and haematobiochemical profiles in healthy donkeys (Equus asinus).

BACKGROUND: The effect of digoxin administration on the serum concentration of the cardiac troponin I (cTnI) has not been reported to date in equidae. This study was therefore designed to evaluate the effect of digoxin on cardiac cell damage in donkeys (Equus asinus) as assessed by cTnI, acid-base and electrolyte balance and haematobiochemical profiles. Ten clinically healthy donkeys were given an IV infusion of digoxin at a dose of 14 µg/kg. Blood samples were collected from the donkeys up through 72 h post-injection. RESULTS: Three of the donkeys exhibited increased heart and respiratory rates post-injection. In the other seven animals, the heart and respiratory rates were lower 4 h post-injection. The serum digoxin concentration increased significantly at many time points after injection. The serum concentration of cTnI did not differ significantly between pre- and post-injection. An increase in blood pH was noted at 3 h after digoxin injection. There were also increases in PO2 and in oxygen saturation. Decreases in PCO2 at 2 to 48 h post-injection as well as a decrease in blood lactate at 4 h post-injection were observed. The serum concentration of glucose remained significantly elevated at all-time points after digoxin injection. CONCLUSIONS: It is concluded that administration of digoxin to healthy donkeys (14 µg/kg) did not result in elevations of serum cTnI concentration, signs of digoxin intoxication, ECG abnormalities and did not increase serum concentrations of blood urea nitrogen and creatinine.

The effect of tick infestation on the serum concentrations of the cardiac biomarker troponin I, acid-base balance and haematobiochemical profiles in camels (Camelus dromedarius).

The aim of the present study was to evaluate the serum concentration of cardiac troponin I (cTnI) in camels with tick infestation as a marker of potential myocardial injury and its prognostication. The effects of acid-base and electrolyte balance and haematobiochemical profiles were also investigated. Twenty-three camels (Camelus dromedarius) with tick infestation and suffering from anorexia, incoordination of movement, unsteady gait, recumbency, opisthotonus, anaemia and reduced production were examined. Ticks were visible, deeply embedded in the skin over the whole body, especially under the neck and around the udder or testis. Of the camels, 15 recovered after treatment and 8 did not. Blood samples were collected from the diseased camels on the day of admission to our clinic. Blood samples were also collected from 12 healthy camels and were used as controls. The mean serum concentration of cTnI in the camels with tick infestation was 1.7 ± 1.6 ng/ml compared to 0.03 ± 0.02 ng/ml in the controls. The mean serum concentration of cTnI in those camels that recovered was 0.36 ± 0.53 ng/ml compared to 3.0 ± 1.1 ng/ml in the camels that did not. Venous blood gas alterations included an increase in partial pressure of carbon dioxide and decreases in partial pressure of oxygen and oxygen saturation. Haematological parameters involved decreases of red blood cells, haemoglobin and haematocrit and increases in the mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Biochemical parameters included significant elevations in the serum activity of aspartate aminotransferase, creatine kinase and alkaline phosphates. Other biochemical alterations observed were decreases in total protein and albumin and increases in globulin and glucose concentrations. In conclusion, the data of this study suggest the possibility of using cTnI as a biomarker for cardiac injury in camels with tick infestation and for the prognosis of the outcome in the treated animals. Generally, values above 1.0 ng/ml were considered bad prognostic indicators.

Cardiac troponin I in healthy newborn goat kids and in goat kids with cardiac nutritional muscular dystrophy.

This study was designed to establish serum cardiac troponin I (cTnI) concentrations in healthy newborn goat kids and in those with cardiac nutritional muscular dystrophy (NMD). Thirty-five single full-term newborn goat kids (20 males and 15 females; age: 6.1 ± 3.5 h; weight 3.4 ± 0.68 kg), together with their respective mothers (Group 1; G1) were enrolled consecutively. Thirty-one goat kids (age: 9.5 ± 4.3 days) with NMD, together with 20 control goat kids (age: 7.8 ± 4.3 days) were also included in this study (Group 2; G2). Blood samples were collected from G1 within 12 h of birth and from G2 on admission. Serum samples were collected and analysed for cTnI. In G1, the mean serum concentration of cTnI in goat kids was 0.290 ± 0.37 ng/mL, with no statistically significant difference between male and female kids (P = 0.61). The mean cTnI concentration in the does was 0.017 ± 0.04, ng/mL. Serum values of cTnI in the goat kids and in their respective mothers differed significantly (P = 0.0001). In G2, the mean cTnI concentration was 0.02 ± 0.05 ng/mL in the control and 11.18 ± 20.07 ng/mL in the diseased goat kids, with a statistically significant difference between diseased and control goat kids (P = 0.017). Serum concentrations of cTnI are higher in goat kids than in their respective mothers. In conclusion, the cTnI assay appears to be a sensitive and specific marker for myocardial injury in goat kids.

Effect of racing on the serum concentrations of cardiac troponin I and creatine kinase myocardial band in racing camels (Camelus dromedarius).

This study was designed to investigate the effect of racing on the serum concentrations of cardiac troponin I (cTnI) and creatine kinase myocardial (CK-MB) in healthy racing camels (Camelus dromedarius). Twenty-three racing camels scheduled for a 5 km race were investigated in this study. From each camel, 3 blood samples were collected: 24 h before racing (T0), within 2 h after the race (T1) and 24 h post-race (T2). Following the 5 km race, 91.3 % of the racing camels had increases in serum cTnI concentrations, while concentrations remained unchanged in 8.7 %. The cTnI concentration (median 0.06 ng/mL; range, 0.03-0.15 ng/mL) was significantly higher (P < 0.001) than the pre-race values (median 0.04 ng/mL; range, 0.01-0.07 ng/mL). Twenty-four hours post-race, the cTnI concentrations had returned very nearly to their pre-race values (median 0.04 ng/mL; range, 0.00-0.09 ng/mL) and were not significantly different (P = 0.35) from the pre-race values. Following the 5 km race, increases in CK-MB mass were seen in 17.4 % of the camels, with no changes in 4.3 % and decreases in 78.3 %. The CK-MB mass (median 0.41 ng/mL; range, 0.19-0.60 ng/mL) did not differ significantly (P = 0.84) when compared to the pre-race values (median 0.42 ng/mL; range, 0.32-0.55 ng/mL). Twenty-four hours post-race, the CK-MB mass concentrations (median 0.41 ng/mL; range, 0.15-0.55 ng/mL) did not differ significantly (P > 0.05) compared to pre-race or immediate post-race values. Resting cTnI concentrations in the racing camels were initially low, but increased above the baseline level in most of the camels immediately after racing, and returned to pre-race values within the 24-h post-race period. CK-MB is a less sensitive biomarker for myocardial activity as compared with cTnI. These findings could be of importance when evaluating racing camels with suspected cardiac disease after recent hard exercise.

Cardiac biomarker changes in camels (Camelus dromedarius) secondary to road transportation.

OBJECTIVES: Little is known about cardiac biomarkers in camels despite their extensive use as draft animals. This study was designed to establish reference ranges for the cardiac biomarkers cardiac troponin I (cTnI) and creatine kinase myocardial b fraction (CK-MB) in healthy camels and to investigate their changes in response to road transportation. ANIMALS: Twenty-five healthy camels transported for a 5 h round-trip journey. METHODS: None of the camels had evidence of cardiac abnormalities on cardiac auscultation, echocardiography or electrocardiography. Three blood samples were obtained from each camel: 24 h before transportation (T0), within 2 h after unloading (T1) and 24 h after transportation (T2). RESULTS: The mean cTnI concentration in the camels was 0.032 ± 0.023 ng/mL. All the camels had resting cTnI concentrations of <0.08 ng/mL. At T1, the cTnI concentration was significantly higher (P < 0.001) in all 25 camels compared to values at T0. The CK-MB concentration in the camels was 0.19 ± 0.05 ng/mL. All the camels had resting CK-MB concentrations of <0.33 ng/mL. At T1, the CK-MB concentration was higher in 3/25 camels compared to values at both T0 and T2. Concerning the hematobiochemical variables, significant increases were detected at T1 in total white blood cells, total protein, globulin, magnesium and phosphorus. Cardiac troponin I, CK-MB and all the hematobiochemical parameters had returned to their pre-transport values at T2. CONCLUSIONS: 5 h road transportation might have transient adverse effects on the cardiac muscle of healthy camels.

Influence of racing on the serum concentrations of the cardiac biomarkers troponin I and creatine kinase myocardial band (CK-MB) in racing greyhounds.

The objective of this study was to investigate the effect of racing on the serum concentrations of cardiac troponin I (cTnI) and creatine kinase myocardial band (CK-MB) in 32 racing greyhounds. Blood samples were collected 24h prior to a 7 km race (T0), within 2h of completion of the race (T1), and 24h post-race (T2). Blood samples were also collected from 20 non-racing greyhounds. The median cTnI concentration in the racing greyhounds was not significantly different from that in the non-racing greyhounds (0.045 ng/mL). Before racing, the median cTnI concentration in the racing greyhounds was 0.050 ng/mL. Following the 7 km race, 31/32 greyhounds showed increases in cTnI concentrations which were significantly higher than the pre-race concentrations (P<0.0001). cTnI concentrations dropped back 24h post-race to values not significantly different from the pre-race concentrations. Following the race, 5/32 greyhounds showed mild increases in CK-MB concentrations but these were not significantly different from the pre-race values. These findings could be of importance when evaluating greyhounds with suspected cardiac disease that have recently performed hard exercise.