Subject(s)
Acne Conglobata/genetics , Amyloid Precursor Protein Secretases/genetics , Hidradenitis Suppurativa/genetics , Membrane Glycoproteins/genetics , Mutation , Acne Conglobata/diagnosis , DNA Mutational Analysis , Family Health , Female , Frameshift Mutation , Gene Expression Profiling , Genetic Association Studies , Hidradenitis Suppurativa/diagnosis , Humans , Male , Phenotype , Receptors, Notch/metabolismABSTRACT
To understand the transmission of Cryptosporidium infection in children, fecal specimens from 62 Kuwaiti children with gastrointestinal symptoms found to be positive by microscopy were genotyped and subtyped with a small subunit rRNA-based PCR-restriction fragment length polymorphism analysis and a 60-kDa glycoprotein-based DNA sequencing tool. The median age of infected children was 4.5 years, and 77% of infections occurred during the cool season of November to April. Fifty-eight of the children (94%) had Cryptosporidium parvum, three (5%) had Cryptosporidium hominis, and one (1%) had both C. parvum and C. hominis. Altogether, 13 subtypes of C. parvum (belonging to four subtype allele families) and C. hominis (belonging to three subtype allele families) were observed, with 92% of specimens belonging to the common allele family IIa and the unusual allele family IId. Thus, the transmission of cryptosporidiosis in Kuwaiti children differed significantly from other tropical countries.
Subject(s)
Cryptosporidiosis/epidemiology , Cryptosporidium/classification , Cryptosporidium/genetics , Endemic Diseases , Adolescent , Adult , Animals , Child , Child, Preschool , Cryptosporidiosis/parasitology , Cryptosporidiosis/transmission , Cryptosporidium/isolation & purification , Cryptosporidium parvum/classification , Cryptosporidium parvum/genetics , Cryptosporidium parvum/isolation & purification , DNA, Protozoan/analysis , Feces/parasitology , Female , Humans , Infant , Kuwait/epidemiology , Male , Molecular Sequence Data , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , RNA, Ribosomal/genetics , Sequence Analysis, DNAABSTRACT
Myiasis, the invasion of live human tissue by larvae of Diptera, is reported in the nasopharynx and a leg wound in two patients who were hospitalized for more than 72 hours in Mubarak Al-Kabeer Teaching Hospital in Kuwait City, Kuwait. On the fourth and fifth days after a 10-year-old Kuwaiti boy was admitted to the hospital intensive care unit in a bloodied and comatose state following a traffic accident, 'worms' that came out of his nostrils were fixed, cleared, and identified as second and third instar of Lucilia sericata (Diptera: Calliphoridae). After 14 days, 'worms' were seen in the original dressing of a 35-year-old Iranian man admitted to the Orthopedic Unit of the hospital with multiple lacerations and fractures. The larvae, in various stages of development, were identified as those of Megaselia scalaris (Diptera: Phoridae). Since the presence of larvae in both patients was recorded after a stay of at least 3-4 days in the hospital, by definition, these infestations are considered nosocomial.
