ABSTRACT
Long noncoding RNAs (lncRNAs) have been reported to be involved in various cellular processes and to participate in a variety of human diseases. Recently, increasing studies have reported that lncRNAs are related to many reproductive diseases, such as pathogenesis of recurrent pregnancy loss (RPL), preeclampsia (PE) and gestational diabetes mellitus (GDM). In this study, we aimed to investigate the effect of LINC01088 in trophoblast cells and its potential role in pathogenesis of RPL. LINC01088 was found to be upregulated in first-trimester chorionic villi tissues from RPL patients. Increased LINC01088 repressed proliferation, migration and invasion of trophoblast cells, and promoted apoptosis of trophoblast cells. Further exploration indicated that LINC01088 decreased the production of nitric oxide (NO) by binding and increasing Arginase-1 and decreasing eNOS protein levels. Importantly, JNK and p38 MAPK-signaling pathways were active after overexpression of LINC01088. In conclusion, our studies demonstrated that LINC01088 plays an important role in the pathogenesis of RPL, and is a potential therapeutic target for the treatment of RPL.
Subject(s)
Abortion, Habitual/genetics , MAP Kinase Signaling System/physiology , RNA, Long Noncoding/genetics , Trophoblasts/metabolism , Abortion, Habitual/physiopathology , Adult , Apoptosis , Arginase/metabolism , CRISPR-Cas Systems , Cell Cycle , Cell Division , Cell Line , Cell Movement , Chorionic Villi/metabolism , Chorionic Villi/pathology , Female , HEK293 Cells , Humans , Nitric Oxide/metabolism , Nitric Oxide Synthase Type III/metabolism , Pregnancy , Pregnancy Trimester, First , RNA, Guide, Kinetoplastida/genetics , RNA, Long Noncoding/biosynthesis , Trophoblasts/pathology , Up-Regulation , Young AdultABSTRACT
At the moment, pancreatic cancer is among the deadliest gastrointestinal diseases, and pancreatic cancer growth is a complex biological process that is based on different kinds of genes. Exosomes are extracellular vesicles containing microRNAs (miRNAs), messenger RNA (mRNA), and proteins, they act as the most prominent mediator of intercellular communication, and they regulate, instruct, and re-educate their surrounding microenvironment and target specific organs. Due to accumulative evidence proved that exosomes are involved in metastasis, cell proliferation, EMT, angiogenesis, and TME of pancreatic cancer, exosomes are crucial potential candidates to detect pancreatic cancer early. This review aims to convey the current understanding of the main functions employed by exosomes in early diagnosis and treatment of pancreatic cancer.
