ABSTRACT
The organ distribution data for the two hepatobiliary agents HIDA and p-butyl-IDA in mice showed substantial differences in the extent and rate of hepatobiliary clearance of radioactivity. 99mTc-HIDA and 99mTc-p-butyl-IDA were used in computer assisted serial hepatobiliary scintigraphy. In normal subjects, the data of dynamic studies showed that the kidneys were no longer seen after 2.5 min with p-butyl-IDA. Liver radioactivity decreased significantly after 10 min with HIDA compared to 35 min with p-butyl-IDA. The gall bladder was always visualized at the 10-12th min and the 25-30th min with HIDA and p-butyl-IDA respectively. 99mTc-HIDA provided superior hepatic duct images.
Subject(s)
Biliary Tract Diseases/diagnostic imaging , Liver Diseases/diagnostic imaging , Organotechnetium Compounds , Animals , Humans , Imino Acids/blood , Kidney/diagnostic imaging , Mice , Protein Binding , Radionuclide Imaging , Technetium/blood , Technetium Tc 99m LidofeninABSTRACT
A freeze-dried powder of tin-p-butyl-IDA to be labelled with 99mTc for hepatobiliary scintigraphy has been developed. Gel chromatography column scanning has been applied for evaluating the labeling efficiency of the complex under different conditions. The results of organ distribution studies in mice and of clinical investigations in human beings showed the stability of the radiopharmaceutical. The preparative 99mTc-p-butyl-IDA concentrates moderately well in the bile with a rapid blood clearance into hepatocytes, very low excretion in the urine and slow clearance into the bile.
Subject(s)
Biliary Tract/diagnostic imaging , Imino Acids/chemical synthesis , Liver/diagnostic imaging , Organotechnetium Compounds , Technetium/chemical synthesis , Animals , Chromatography, Gel , Humans , Hydrogen-Ion Concentration , Imino Acids/metabolism , Kinetics , Mice , Rabbits , Radionuclide Imaging , Technetium/metabolism , Time Factors , Tissue DistributionABSTRACT
A rapid method for the production of freeze-dried kit containing 20 mg HIDA and 0.4 mg SnCl2 X 2H2O at pH 5.5-6.0 was developed using the stannite procedure. The Sn-HIDA ligand has been prepared successfully by dissolving HIDA in stannite solution without further pH adjustment. A gelchromatography column scanning (GCS) technique has been applied to study the chemical state of technetium in the preparation. The shelf-life of the freeze-dried powder of Sn-HIDA was found stable for at least 6 months as assessed by the GCS-technique. The hepatobiliary agent has a rapid blood clearance and is excreted predominantly by the hepatobiliary system. The biodistribution study in mice showed that more than 30% of the injected dose has been accumulated in liver at 5 min postinjection and at 30 min most of the injected activity has been passed to the intestine via the biliary system.
Subject(s)
Imino Acids , Technetium , Tin Compounds , Animals , Drug Stability , Imino Acids/blood , Imino Acids/metabolism , Indicators and Reagents , Isotope Labeling , Mice , Rabbits , Reagent Kits, Diagnostic , Technetium/blood , Technetium/metabolism , Technetium Tc 99m Lidofenin , Tin , Tissue DistributionABSTRACT
A new radiopharmaceutical for liver scintigraphy has been prepared by reacting 169YbOCl with Na phytate under conditions which yield a labelling efficiency of more than 99% over a wide pH range. In rabbits the resultant colloidal preparation shows a rapid elimination from the blood and high initial uptake in liver. Subsequently about 90% of the liver activity is lost within 3 days, the remainder being eliminated more slowly. The radiopharmaceutical is non-toxic and has a shelf-life of about 3 months. Scintigrams obtained in patients with hepatomegaly suggest that this new agent gives better visualization of space occupying lesions of the liver than 99mTc-Sn colloid. The radiation dose to the patient from this agent is low and comparable with that from other radiopharmaceuticals used for liver scintigraphy.
Subject(s)
Liver/diagnostic imaging , Phytic Acid , Technetium Compounds , Tin Compounds , Ytterbium , Adult , Animals , Hepatomegaly/diagnostic imaging , Humans , Male , Rabbits , Radiation Dosage , Radionuclide Imaging , Technetium , Tin , Tissue DistributionABSTRACT
99mTc-Cu-EHDP has been prepared with high labelling yield applying for the first time the method of instantly formed cuprous ions in the mixture. A gel chromatography column scanning technique has been used to study the 99mTc fractions in the preparation. The study of the influence of pH-value on the amount of 99mTc-CU-EHDP fraction shows that pH 1.6 - 1.7 gave the best labelling results. The formation rate of 99mTc-Cu-EHDP complex with a high labelling yield was fast and achieved within a few mins. This suggests the reduction of 99mTc-pertechnetate to Tc (IV). The final preparation was found stable for at least 4 hrs after mixing the reactants with the 99mTc-eluate. Comparative biokinetic studies of 99mTc-Cu-EHDP and 99mTc-Sn-EHDP in rabbits and mice showed a high bone uptake and fast elimination of 99mTc-Cu-EHDP from the skeleton. No significant difference was found in the plasma protein binding of 99mTc-Cu-EHDP and 99mTc-Sn-EHDP in rats as assessed by the GCS-technique. Radionuclide imaging in rabbits, using a gamma camera, showed 99mTc-Cu-EHDP to be a good bone-imaging agent.
