ABSTRACT
Kits were developed for the sterile labelling of Human Albumin Microspheres with 99mTc. The microspheres were prepared, sieved to get the desired particle size and autoclaved for sterilization. The spheres were treated with stannous chloride and the pH of the suspension was adjusted to 3.7 with phosphate buffer. After freeze-drying the contents of single reaction vials from different batches were reacted with 99mTc and the radiochemical yield (higher than 97%) was determined. The HAM kits were stable and the stability of [99mTc]HAM was followed for 5 h. Lung and liver uptake in mice was determined to be about 90 and 1% respectively. The preparation of [99mTc]HAM is performed in a single step process and excellent human lung scans were obtained.
Subject(s)
Lung/diagnostic imaging , Serum Albumin , Technetium , Adult , Animals , Drug Stability , Humans , Isotope Labeling/methods , Male , Mice , Radionuclide Imaging , Reagent Kits, Diagnostic , Serum Albumin/metabolism , Technetium/metabolism , Technetium Tc 99m Aggregated Albumin , Tissue DistributionABSTRACT
Kits were developed for the sterile labelling of macroaggregated albumin (human) with 99mTc. 0.4 mL of 20% HSA, 1 mL of SnCl2 X 2H2O containing 5 mg and 1.1 mL of 0.9% NaCl were sonified for 10 min. The pH was adjusted with phosphate buffer to 5.5 after the addition of 1 mL of 5% Tween-80. The solution was heated with stirring for 10 min at 80 degrees C for aggregation and 1.0 mL portions were placed in 10 mL vials for lyophilization. The contents of single reaction vials were reacted with 99mTc and the radiochemical yield determined (higher than 98%). The MAA kits were stable and the stability of [99mTc]-MAA was followed for 4.5 h. Lung uptake in mice was determined to be about 98.5%. The preparation of [99mTc]-MAA was performed in a single step process and excellent human lung scans were obtained.
