Molecular characterization of most cultivated Pleurotus species in sub-western region Nigeria with development of cost effective cultivation protocol on palm oil waste.

Major problems associated with the study of mushrooms in Nigeria are inaccuracy in identification, low bio-efficiency of commonly used substrate and difficulty in composting of substrates. The current study attempts the identification and characterization of Pleurotus species commonly cultivated in Western region of Nigeria, and evaluates the effectiveness of agricultural wastes in mushrooms cultivation. Molecular characterization was carried out for species identification. Different substrates combinations at ratio 1:1 were used. Molecular characterization revealed that the Pleurotus species grown in most farms and research institutes in South-western Nigeria are predominantly P. ostreatus and P. pulminarius. Palm bunch + Rice bran (8.24 ± 0.16) ramified almost twice faster than sawdust alone (4.98 ± 0.31) or any of the other substrates containing it. Fermented bunch (7.36 ± 0.19), the only substrate not sterilized also ramified faster than sawdust alone. All the other substrates compounded with palm bunch and shaft ramified faster than sawdust as lone substrate. In terms of yield, Palm bunch + Rice bran gave the highest (1774.75 g), followed by shaft + rice bran (1483.70 g), while the least value of 326. 94 g was obtained from sawdust. The highest value of biological efficient (BE) (100.57 g) and productivity (PT) (17.46 g) were obtained from shaft + Wheat bran and Palm bunch + Rice bran respectively, while sawdust gave lowest values of 13.08% and 3.23% for BE and PT respectively. Results obtained have shown that bunch and shaft supplemented with wheat and rice bran gave better yields and can be recommended for commercial mushrooms cultivation.

Limited cross reactivity among arginine kinase allergens from mealworm and cricket edible insects.

Insects are seen as a solution to the increasing demand for protein sources for food. However, entomophagy has unfortunately been linked to allergic reactions in Europe with people with professional contacts. As mealworms (Tenebrio molitor) and crickets (Acheta domesticus) have recently become commercially available (both whole or in food formulation) in several European countries, this research assessed the cross allergenicity of arginine kinase (AK). Based on the collection of sera from a entomology laboratory staff, oven cooked insects but also purified AK fractions were tested. Immunoblotting against the protein extracts revealed different Immunoglobulin E reactivity of sera according to the insect target species: two bands (40 and 14 kDa) for crickets and a pattern including light responses at 17, 25 and 37 kDa for mealworms. Focusing on AK, low specific allergenicity was here illustrated and discussed in relation to the development of a safe edible insect consumption by humans.

Ameliorative effects of ß-sitosterol on some biochemical indices of hypertension in wistar albino rats.

BACKGROUND: Cardiovascular diseases presently rank high as leading causes of death globally. The increasing acceptability of phytomedicine is due to the increasing inefficacy of many modern drugs used for the control of many diseases. The aim of this study was to investigate the ameliorative effects of ß-sitosterol (BSS) in comparison with lisinopril, a standard antihypertensive drug, on certain biochemical hypertensive parameters in rats. METHODS: Hypertension was induced with cadmium chloride and biochemical analysis of serum was carried out following treatment with BSS and lisinopril. Serum urea, creatinine and electrolytes were assayed using standard kit as tests for renal function, while alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine aminotransferase (ALT) served as enzyme indices of the liver function. The effect on the serum lipid profile was assessed and histological examination performed on tissues of the liver and kidney. RESULTS: The rats treated with BSS showed a significant decrease (p<0.05) in the serum creatinine concentration when compared with the hypertensive rats. Treatment with lisinopril showed a significant increase (p<0.05) in the activity of AST and ALP when compared with the normal rats. There were slight variations in the concentration of serum electrolytes of rats treated with BSS and lisinopril respectively when compared with normal and hypertensive rats. BSS significantly reduced calcium levels when compared with the hypertensive group. The histopathological examination of the liver and kidney of animals treated with BSS was not different from the control which showed normal histological structure, while the liver of the hypertensive animals showed scanty inflamed cells. CONCLUSION: The study shows that BSS is effective in restoring basal liver and kidney functions in hypertensive rats.

Identification of taste receptors and proteomic characterization of the antenna and legs of Tribolium brevicornis, a stored food product pest.

Chemoreception plays an important role in mediating a diverse range of behaviours, including predation and food selection. In the present study, we combined anatomical observations, electrophysiology and proteomics to investigate sensilla that mediate chemoreception on the antenna and the legs of Tribolium. Scanning electron microscopy was used to differentiate the coxal and trochanteral segments of the pro-, meso- and metathoracic legs by the presence of sensilla trichoidea and chaetica, while the antennae were covered with five types of sensilla (chaetica, basiconica, trichoidea, squamiformia and coeloconica). Antenna morphology and ultrastructure were similar in both sexes. Electrophysiological recordings allowed us to characterize a row of small sensilla basiconica on the terminal segment of the antenna as taste receptors, responding to sucrose and NaCl. Proteomics investigations of antennae and legs yielded several proteins with specific interest for those involved in chemoreception. Odorant-binding proteins were antenna-specific, while chemosensory proteins were detected in both tissues.

Modulation of opening of rat liver mitochondrial membrane permeability transition pore by different fractions of the leaves of Cnestis ferruginea. D.C.

BACKGROUND: Increased attention is now directed towards the search for novel naturally occurring anticancer agents that can induce mitochondrial membrane permeability transition (MMPT) pore opening and cell death as a chemotherapeutic mechanism to combat cancer incidence. AIM: The inductive effects of partially purified fractions of leaves of Cnestis ferruginea- on rat liver MMPT pore opening was investigated. METHOD: De-fatted methanol extract of leaves of Cnestis ferruginea was partitioned between water, chloroform, ethylacetate, or butanol separately in succession. The extract solutions were concentrated at 40 degrees C to obtain water (WF), chloroform (CF), ethylacetate (EF) and butanol (BF) fractions. The effects of these fractions (0.2- 1.4 mg/ml) on MMPT pore opening or mitochondrial swelling in the presence and absence of calcium were evaluated The effects of these fractions on the rat liver mitochondrial F0F1-ATPase activity were also assessed. RESULTS: Ca(2+)-induced MMPT pore opening was inhibited by 1 mg/ml each of MECF, CF, BF, WF and EF by 75.0%, 83.0%, 88.0%, 68.0%, and 71.0%, respectively and compared with the effect of spermine, a standard inhibitor. However, in the absence of Ca2+, the fractions significantly induced MMPT pore opening in intact mitochondria by 7.0, 5.7, 0.7, 4.8, 10.9 folds, respectively. In normal rat liver mitochondria, F1F0-ATPase activity was stimulated maximally by MECF, CF, EF, BF and WF by 4.7, 12.7, 1.6, 3.6 and 1.5 folds, respectively, thus indicating that the chloroform fraction is the most potent and therefore contains the active principle in the plant. CONCLUSION: The present study revealed that the leaves of Cnestis ferruginea contain bioactive substances that induced mitochondrial membrane permeability transition and activated the specific activity of F0F1 ATPase. Thus, suggesting strongly that these bioactive agents may serve as a useful chemotherapeutic strategy in cancer therapy.

Does Tribolium brevicornis cuticular chemistry deter cannibalism and predation of pupae?

The cuticular hydrocarbons of insects are species-specific and often function as semiochemicals. The activity of Tribolium brevicornis cuticular hydrocarbons as feeding deterrents that ostensibly function to prevent pupal cannibalism and predation was evaluated. The cuticular hydrocarbons of T. brevicornis pupae were characterized and flour disk bioassays conducted with individual and combined extract components incorporated into artificial diets on which Tribolium adults fed for six days. Feeding by T. brevicornis and T. castaneum on flour disks containing cuticular extracts of T. brevicornis pupae resulted in reduced consumption and weight loss relative to feeding on control flour disks. In both cases, feeding deterrence indices exceeded 80% suggesting that T. brevicornis cuticular hydrocarbons could function to deter cannibalism and predation of pupae by larvae and adult beetles. Sixteen different cuticular hydrocarbons were identified in T. brevicornis pupal extracts. Eight of the commercially available linear alkanes were tested individually in feeding trials with eight Tribolium species. One compound (C28) significantly reduced the amount of food consumed by three species compared to control disks, whereas the compounds C25, C26, and C27 elicited increased feeding in some species. Four other compounds had no effect on consumption for any species. When four hydrocarbon mixtures were tested for synergistic deterrence on T. brevicornis and T. castaneum, none significantly influenced consumption. Our results indicate that the cuticular chemistry of T. brevicornis pupae could serve to deter predation by conspecific and congeneric beetles.

The impact of a UNICEF-assisted rural water project on the prevalence of guinea worm disease in Asa, Kwara State, Nigeria.

This paper demonstrates that protected water supplies, in the form of boreholes, can reduce the prevalence of dracunculiasis (guinea worm disease) in affected communities from a point prevalence of greater than or equal to 50% to 0% or near 0% within 3 years of intervention. Studies in Nigeria show that boreholes sited within a village and used exclusively for drinking water are most effective, while less accessible or malfunctioning boreholes have a less dramatic impact on prevalence. In contrast to the situation in villages served with boreholes, the prevalence of guinea worm in the unserved villages remained almost unchanged. The rapid benefits of protected rural water supplies and the decline of dracunculiasis, including the rise in school enrollment and fall in the rate of school absenteeism, are highlighted together with other socioeconomic benefits.

'Leopard skin' as a rapid diagnostic index for estimating the endemicity of African onchocerciasis.

A study was made in five rural districts of Kwara State, Nigeria to assess the possible use of 'leopard skin' (Ls) as a rapid diagnostic technique for estimating the endemicity of African onchocerciasis. The findings revealed that there is a positive association between the percentage of subjects with skin microfilariae (mf) and the percentage with Ls. The prevalence of Ls less than 1% suggests communities with sporadic infection, 1-6% suggests hypoendemic communities and greater than 6% suggests meso- or hyperendemic communities, using mf rates of less than 10%, 10-39%, 40-69% and 70% or greater as the standard to classify sporadic, hypoendemic, mesoendemic and hyperendemic levels of the infection, respectively. The unique merits of Ls as a potential onchocercal index are presented.