ABSTRACT
Keloids are the result of aberrant wound healing of human skin after dermal injury. Therapeutic options include excision followed by radiation therapy, steroid injection, and compression with silicone sheets among others. Local invasion and recurrence after excision has provoked interest in treating keloids as neoplasms. The purpose of this study was to determine the effect of mitomycin C (MMC) on keloid fibroblasts. Keloid fibroblasts obtained from five different patients were exposed to MMC. A control group of normal and keloid cells was treated with phosphate buffered saline only. Contrast microscopy showed a decrease of fibroblast density during the 3 weeks after exposure for normal and keloid fibroblasts relative to untreated fibroblasts. This was confirmed by total cell counts ( = 0.1) and measurement of DNA synthesis. By the third week, there was a recovery in DNA synthesis and increased cell count for some of the treated fibroblasts. We concluded that at an appropriate concentration, MMC shows proliferation of keloid fibroblasts in vitro for a period of 3 weeks. This agent may be considered in clinical trials after surgical excision of keloids.
Subject(s)
Cross-Linking Reagents/pharmacology , Fibroblasts/drug effects , Keloid/pathology , Mitomycin/pharmacology , Nucleic Acid Synthesis Inhibitors/pharmacology , Cell Division/drug effects , Cell Line , Cell Survival/drug effects , Cells, Cultured , DNA/biosynthesis , Fibroblasts/metabolism , Humans , Keloid/metabolismABSTRACT
An unusual case of a patient who presented with a subcutaneous soft tissue lesion of the back is described. The patient had no preceding history of cutaneous malignancy or local trauma. Excision of the lesion revealed a diagnosis consistent with basal cell carcinoma with deep infiltration. The site was re-excised with a generous margin to ensure complete removal. There has been no recurrence for 18 months. This case is presented to underscore the rarity and importance of its existence.
Est décrit le cas inhabituel d'un patient qui s'est présenté avec une lésion des tissus mous sous-cutanés dans le dos. Le patient ne présentait aucun antécédent de malignité cutanée ou de traumatisme local. L'excision de la lésion a révélé un diagnostic confirmant la présence d'un carcinome basocellulaire avec infiltration profonde. Une nouvelle excision, comportant une marge plus importante, a été effectuée au foyer de la lésion afin d'en garantir l'extraction complète. Aucune récurrence ne s'est manifestée depuis 18 mois. Ce cas est présenté pour en souligner la rareté et l'importance.
