Inactivation of Escherichia coli, Salmonella enterica, and Listeria monocytogenes using the Contamination Sanitization Inspection and Disinfection (CSI-D) device.

The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a handheld fluorescence-based imaging system designed to disinfect food contact surfaces using ultraviolet-C (UVC) illumination. This study aimed to determine the optimal CSI-D parameters (i.e., UVC exposure time and intensity) for the inactivation of the following foodborne bacteria plated on non-selective media: generic Escherichia coli (indicator organism) and the pathogens enterohemorrhagic E. coli, enterotoxigenic E. coli, Salmonella enterica, and Listeria monocytogenes. Each bacterial strain was spread-plated on non-selective agar and exposed to high-intensity (10 mW/cm2) or low-intensity (5 mW/cm2) UVC for 1-5 s. Control plates were not exposed to UVC. The plates were incubated overnight at 37 °C and then enumerated. Three trials for each bacterial strain were conducted. Statistical analysis was carried out to determine if there were significant differences in bacterial growth between UVC intensities and exposure times. Overall, exposure to low or high intensity for 3-5 s resulted in consistent inhibition of bacterial growth, with reductions of 99.9-100 % for E. coli, 96.8-100 % for S. enterica, and 99.2-100 % for L. monocytogenes. The 1 s exposure time showed inconsistent results, with a 66.0-100 % reduction in growth depending on the intensity and bacterial strain. When the results for all strains within each species were combined, the 3-5 s exposure times showed significantly greater (p < 0.05) growth inhibition than the 1 s exposure time. However, there were no significant differences (p > 0.05) in growth inhibition between the high and low UVC intensities. The results of this study show that, in pure culture conditions, exposure to UVC with the CSI-D device for ≥3 s is required to achieve consistent reduction of E. coli, S. enterica, and L. monocytogenes.

COVID-19, body weight and the neighbourhood: food system dimensions and consumption associated with changes in body weight of Peruvian adults during first wave lockdowns.

Objective: The objective of this study is to assess changes in the dimensions of the food system and consumption associated with body weight variations during the first month's lockdown in Peruvian adults in Metropolitan Lima. Methods: A cross-sectional study conducted during the first months of lockdowns in Peru. 694 adults completed a web-based survey about changes experienced in the process of acquiring food during lockdown, changes in their intake and self-perceived body weight. A multinomial logistic regression analysis was conducted to evaluate the factors associated with changes in body weight. Results: Weight gain was perceived in 38% of the participants and 22.8% perceived weight loss. 39.2% did not perceive changes in their weight. Risk factors for body weight gain were increased alcohol consumption (OR=4.510, 95% CI 1.764 to 11.531) and decreased fruit consumption (OR=2.129, 95% CI 1.290 to 3.515), while decreasing cereal intake (OR=0.498, 95% CI 0.269 to 0.922) and choosing nutritious food as a driver for purchase (OR=0.512, 95% CI 0.320 to 0.821) were found to be protective against gaining weight. Decreasing food intake during the pandemic (OR=2.188, 95% CI 1.348 to 3.550) and having to miss important foods (OR=2.354, 95% CI 1.393 to 3.978), were associated with weight loss. Conclusions: During confinement, weight gain was mostly associated with food consumption and personal food system factors. Meanwhile, weight loss was associated with external food system factors.

Probabilidad de lesión de tren inferior e índice de grasa corporal en talleres deportivos universitarios / Probability of lower body injury and body fat index in university sports workshops

El objetivo es verificar si existe relación entre la probabilidad de lesión en extremidades del tren inferior y el índice de grasa corporal en estudiantes que participan de talleres deportivos en la Universidad Adventista de Chile. Participaron 66 alumnos entre las áreas deportivas voleibol, básquetbol, fútbol y hándbol por un método de muestreo censal. Todos los participantes de la investigación fueron evaluados por medio del instrumento "Y balance Test", para verificar si son propensos a sufrir una lesión de tren inferior y además se les realizó una evaluación antropométrica para conocer su índice de grasa corporal. Los resultados no encontraron correlación entre porcentaje de grasa y probabilidad de lesión, sin embargo, se encontró correlación entre probabilidad de lesión entre una pierna y otra

The objective is to verify if there is a relationship between the likelihood of limb injury in the lower body and the body fat index in students participating in sports workshops at the Adventist University of Chile. 66 students participated in sports areas such as volleyball, basketball, soccer and handball by a census sampling method. All the participants of the investigation were evaluated by means of the "Y balance Test" instrument, to verify if they are prone to suffer a lower train injury and also an anthropometric evaluation was done to know their body fat index. The results did not find correlation between fat percentage and injury probability, however, a correlation was found between the injury probability between one leg and the another

Does fatigue alter step characteristics and stiffness during running?

BACKGROUND: Describing the response of spatiotemporal gait characteristics, and related variables such as variability and stiffness, to different stressors is important to better understand spring-mass model. RESEARCH QUESTION: This study aimed to examine the effect of fatigue induced by a running protocol on spatiotemporal gait parameters, step variability and vertical (Kvert) and leg stiffness (Kleg) during running on a treadmill. METHODS: Twenty-two trained male endurance runners performed a 60-min time trial run. An analysis of spatiotemporal parameters (contact time [CT], flight time [FT], step frequency [SF] and step length [SL]), step variability (in terms of coefficient of variation [CV]) and stiffness was conducted in two different conditions: non-fatigued (before the protocol) and fatigued (after the protocol). RESULTS: The pairwise comparisons (i.e., non-fatigued vs. fatigued condition) indicated that temporal parameters (i.e., CT and FT) experienced significant changes (p = 0.001 and <0.001, respectively). Step variability increased in presence of fatigue, with higher CV in CT (p = 0.039), FT (p = 0.005), SF (p = 0.046) and SL (p = 0.027) after the running protocol. The Kleg experienced a reduction in the fatigued condition (p < 0.001) whereas the Kvert remained unchanged (p = 0.602). SIGNIFICANCE: The results indicate that fatigue induced by a 60-min time trial run causes some adaptations in spatiotemporal gait characteristics and stiffness in trained endurance runners. Specifically, in the presence of fatigue, the athletes showed greater CT and shorter FT, higher step variability and lower leg stiffness.

Efectos del ejercicio físico intervalado en la mejora del control glicémico de adultos obesos con insulinorresistencia / Effects of interval exercise in the improvement of glycemic control of obese adults with insulin resistance

Introducción: el ejercicio físico presenta evidencia para el tratamiento de la resistencia a la insulina. Sin embargo, es necesario profundizar en base a estos conocimientos. Objetivo: comparar la efectividad de un programa de entrenamiento intervalado de alta intensidad (HIIT) con uno de resistencia muscular (RT) para mejorar parámetros bioquímicos de insulina/glicemia basal y poscarga. Material y métodos: se estudiaron 28 personas (edad 36 ± 13 años) insulinorresistentes no medicadas. Se formaron aleatoriamente dos grupos: grupo RT (n = 14) y grupo HIIT (n = 14). Cada grupo participó de 12 semanas de intervención (tres sesiones/semana). Ambos grupos fueron homogéneos (p > 0,05) en cuanto a edad, peso, talla e índice de masa corporal (IMC). La glicemia/insulinemia basal y poscarga igual fueron evaluadas pre- y posintervención. Resultados: tras la intervención existieron disminuciones significativas en ambos grupos en grasa (%), HIIT (Pre = 40,20 ± 7,31 vs. Post = 36,49 ± 7,28%, p = 0,006), RT (Pre: 39,04 ± 8,52 vs. Post: 34,91 ± 8,80%, p = 0,002); en insulina en ayunas HIIT (Pre: 20,64 ± 9,44 vs. Post: 15,20 ± 6,47 uIU/ml, p = 0,0006), RT (Pre: 18,50 ± 8,24, vs. Post: 13,59 ± 6,11 uIU/ml, p = 0,015); en insulina post carga, HIIT (Pre: 127,57 ± 71,73 vs. Post: 69,25 ± 39,42 uIU/ml, p < 0,0001), RT (Pre: 125,78 ± 59,85 vs. Post: 63,45 ± 36,44 uIU/ml, p < 0,0001); y en glicemia en ayunas, HIIT (Pre: 92,86 ± 11,39 vs. Post: 87,36 ± 8,00 mg/dl, p = 0,031), RT (Pre: 90,79 ± 11,26 vs. Post: 85,26 ± 7,88 mg/dl, p = 0,045). En relación a la glicemia poscarga, solo el grupo HIIT disminuyó significativamente (Pre: 128,57 ± 26,90 vs. Post: 103,47 ± 12,70 mg/dl, p < 0,001), reportando diferencias con el grupo RT (p < 0,042). Conclusión: ambas metodologías de trabajos muestran similares resultados para el tratamiento de la insulinorresistencia

Background: physical exercise presents evidence for the treatment of insulin resistance. However, it is necessary to deepen this knowledge. Objective: to compare the effectiveness of a high intensity interval training program (HIIT) with one of resistance training (RT) to improve biochemical parameters of insulin/basal glycemia and post-load. Material and methods: twenty-eight (36 ± 13 years old) non-medicated insulin-resistant individuals (age 36 ± 13 years) were studied. Two groups were randomly formed: RT group (n = 14) and HIIT group (n = 14). Each group participated in 12 weeks of intervention (three sessions/ week). Both groups were homogeneous (p > 0.05) in terms of age, weight, height and BMI. Basal glycemia/insulinemia and post-load were evaluated, pre and post intervention. Results: after the intervention there were significant decreases in both groups in: fat (%) HIIT (Pre = 40.20 ± 7.31 vs Post = 36.49 ± 7.28%, p = 0.006), RT (Pre: 39.04 ± 8.52 vs Post: 34.91 ± 8.80%, p = 0.002); fasting insulin, HIIT (Pre: 20.64 ± 9.44 vs. Post: 15.20 ± 6.47uIU/ml, p = 0.0006), RT (Pre: 18.50 ± 8.24, vs Post: 13.59 ± 6.11 uIU/ml, p = 0.015); insulin post load, HIIT (Pre: 127.57 ± 71.73 vs Post: 69.25 ± 39.42 uIU/ml, p < 0.0001), RT (Pre: 125.78 ± 59.85 vs Post: 63.45 ± 36.44uIU/ml, p < 0.0001); and fasting glycemia, HIIT (Pre: 92.86 ± 11.39 vs Post: 87.36 ± 8.00, p = 0.031), RT (Pre: 90.79 ± 11.26 vs Post: 85.26 ± 7.88 mg/dl, p = 0.045). In relation to post-load glycemia only the HIIT group decreased significantly (Pre: 128.57 ± 26.90 vs Post: 103.47 ± 12.70 mg/dl, p < 0.001), reporting differences with the RT group (p < 0.042). Conclusion: both programs showed similar results for the treatment of insulin resistance

[Effects of interval exercise in the improvement of glycemic control of obese adults with insulin resistance]. / Efectos del ejercicio físico intervalado en la mejora del control glicémico de adultos obesos con insulinorresistencia.

INTRODUCTION: Background: physical exercise presents evidence for the treatment of insulin resistance. However, it is necessary to deepen this knowledge. Objective: to compare the effectiveness of a high intensity interval training program (HIIT) with one of resistance training (RT) to improve biochemical parameters of insulin/basal glycemia and post-load. Material and methods: twenty-eight (36 ± 13 years old) non-medicated insulin-resistant individuals (age 36 ± 13 years) were studied. Two groups were randomly formed: RT group (n = 14) and HIIT group (n = 14). Each group participated in 12 weeks of intervention (three sessions/week). Both groups were homogeneous (p > 0.05) in terms of age, weight, height and BMI. Basal glycemia/insulinemia and post-load were evaluated, pre and post intervention. Results: after the intervention there were significant decreases in both groups in: fat (%) HIIT (Pre = 40.20 ± 7.31 vs Post = 36.49 ± 7.28%, p = 0.006), RT (Pre: 39.04 ± 8.52 vs Post: 34.91 ± 8.80%, p = 0.002); fasting insulin, HIIT (Pre: 20.64 ± 9.44 vs. Post: 15.20 ± 6.47uIU/ml, p = 0.0006), RT (Pre: 18.50 ± 8.24, vs Post: 13.59 ± 6.11 uIU/ml, p = 0.015); insulin post load, HIIT (Pre: 127.57 ± 71.73 vs Post: 69.25 ± 39.42 uIU/ml, p < 0.0001), RT (Pre: 125.78 ± 59.85 vs Post: 63.45 ± 36.44uIU/ml, p < 0.0001); and fasting glycemia, HIIT (Pre: 92.86 ± 11.39 vs Post: 87.36 ± 8.00, p = 0.031), RT (Pre: 90.79 ± 11.26 vs Post: 85.26 ± 7.88 mg/dl, p = 0.045). In relation to post-load glycemia only the HIIT group decreased significantly (Pre: 128.57 ± 26.90 vs Post: 103.47 ± 12.70 mg/dl, p < 0.001), reporting differences with the RT group (p < 0.042). Conclusion: both programs showed similar results for the treatment of insulin resistance.

INTRODUCCIÓN: Introducción: el ejercicio físico presenta evidencia para el tratamiento de la resistencia a la insulina. Sin embargo, es necesario profundizar en base a estos conocimientos. Objetivo: comparar la efectividad de un programa de entrenamiento intervalado de alta intensidad (HIIT) con uno de resistencia muscular (RT) para mejorar parámetros bioquímicos de insulina/glicemia basal y poscarga. Material y métodos: se estudiaron 28 personas (edad 36 ± 13 años) insulinorresistentes no medicadas. Se formaron aleatoriamente dos grupos: grupo RT (n = 14) y grupo HIIT (n = 14). Cada grupo participó de 12 semanas de intervención (tres sesiones/semana). Ambos grupos fueron homogéneos (p > 0,05) en cuanto a edad, peso, talla e índice de masa corporal (IMC). La glicemia/insulinemia basal y poscarga igual fueron evaluadas pre- y posintervención. Resultados: tras la intervención existieron disminuciones significativas en ambos grupos en grasa (%), HIIT (Pre = 40,20 ± 7,31 vs. Post = 36,49 ± 7,28%, p = 0,006), RT (Pre: 39,04 ± 8,52 vs. Post: 34,91 ± 8,80%, p = 0,002); en insulina en ayunas HIIT (Pre: 20,64 ± 9,44 vs. Post: 15,20 ± 6,47 uIU/ml, p = 0,0006), RT (Pre: 18,50 ± 8,24, vs. Post: 13,59 ± 6,11 uIU/ml, p = 0,015); en insulina post carga, HIIT (Pre: 127,57 ± 71,73 vs. Post: 69,25 ± 39,42 uIU/ml, p < 0,0001), RT (Pre: 125,78 ± 59,85 vs. Post: 63,45 ± 36,44 uIU/ml, p < 0,0001); y en glicemia en ayunas, HIIT (Pre: 92,86 ± 11,39 vs. Post: 87,36 ± 8,00 mg/dl, p = 0,031), RT (Pre: 90,79 ± 11,26 vs. Post: 85,26 ± 7,88 mg/dl, p = 0,045). En relación a la glicemia poscarga, solo el grupo HIIT disminuyó significativamente (Pre: 128,57 ± 26,90 vs. Post: 103,47 ± 12,70 mg/dl, p < 0,001), reportando diferencias con el grupo RT (p < 0,042). Conclusión: ambas metodologías de trabajos muestran similares resultados para el tratamiento de la insulinorresistencia.

Desarrollo motor grueso y estado nutricional en niños preescolares con presencia y ausencia de transición I, que cursan el nivel de transición II / Gross motor development and nutritional status among preschool children with presence and absence in transition level I, going through transition level II

El objetivo es evaluar y comparar el desarrollo motor grueso y el estado nutricional, entre preescolares con pre-sencia y ausencia de transición I, que cursan el nivel de transición II. El método: muestra total de 39 niños(as), en edades de 4 y 5 años, correspondiente a un diseño no experimental, descriptivo, transversal, con un enfoque cuantitativo. El estado nutricional, se calculó por medio del índice de masa corporal y para las habilidades motoras gruesas se utilizó la batería motriz, TGMD-2. Los resultados en cuanto al desarrollo motor grueso, presentan dife-rencias a favor del grupo con presencia de transición I en las pruebas de subtest locomotor y de control de objetos; galopar, salto, batear una bola estacionaria, drible estacionario y tomar el balón, las cuales no alcanzan a ser esta-dísticamente significativas en el coeficiente motor total entre ambos grupos. Con respecto al estado nutricional, un 55% de quienes cursaron transición I se encuentran entre sobrepeso y obesidad, mientras que un 26.3% de quienes no lo cursaron se encuentran en esta categoría. Conclusión: en el estado nutricional ambos grupos obtuvieron va-lores similares, quienes cursaron el nivel transición I obtienen mejores resultados en algunas pruebas, diferencias que no alcanzan a ser estadísticamente significativas.

The objective of this study is to evaluate and compare gross motor development and nutritional status among preschool children with presence and absence in transition level I, going through transition level 2. Method: A total sample of 39 children, aged between 4 and 5 years old, corresponding to a non-experimental, descriptive, cross-sectional design with a quantitative approach. The measurement of the nutritional status was calculated by the use of the body mass index (B.M.I) and gross motor skills by the use of a motor battery, TGMD-2. Results: as for gross motor development, there are differences which favor the group with transition I presence in the locomo-tive and object control subtests; galloping, jumping, hitting a stationary ball, steady dribbling and taking the ball, which cannot reach a statistically significant level in regards to the total motor coefficient between both groups. With regards to nutritional status, 55% of the students who went through transition I are found in the overweight and obese category, while 26.3% of those who did not go through it are in this category. Conclusion: In terms of the nutritional status, both groups obtained similar values; those who went through the level I transition obtained better results in some tests. These are differences which cannot be considered statistically significant.

Estudio comparativo entre el desarrollo psicomotor y el estado nutricional en niños de kínder, pertenecientes a un establecimiento municipal y a uno particular de la ciudad de Temuco / Comparative study between psychomotor development and nutritional status of kindergarten children from a municipal and a private educational institution in the city of Temuco, Chile

El presente estudio tiene como objetivo determinar si existen diferencias significativas entre el desarrollo psicomotor y el estado nutricional en niños de 4 a 5 años, pertenecientes a un establecimiento educacional municipal y niños pertenecientes a un establecimiento particular. El diseño es no experimental, transversal y correlacional. El enfoque es de tipo cuantitativo/racionalista. La población está compuesta por 54 niños y niñas de kínder. Para evaluar el desarrollo psicomotor se utilizó el test de Jack Capon, adaptación de Sergio Carrasco (1989). Para conseguir el estado nutricional se obtuvo el peso y la talla de la población. Los resulta-dos indican que los estudiantes del colegio particular, presentaron diferencias significativas en el desarrollo psicomotor, frente a los resultados de los de la escuela municipal. Así también los sujetos con sobrepeso u obesidad, categorizados en malnutrición por exceso presentaron un rendimiento motor inferior en com-paración con los normopeso y bajo peso y en el ámbito de la imagen corporal a mayor peso es menor el reconocimiento de ella.

The aim of this research is to determine whether there are significant differences between psychomotor development and nutritional status in 4 to 5 year-old children that attend a municipal school and those that attend a private one. The design is non-experimental, cross-sectional and correlational and the approach is quantitative/rationalist. The sample is made-up of 54 boys and girls in kindergarten. To evaluate psychomo-tor development, the Jack Capone Test, adapted by Sergio Carrasco (1989), was used. With regards to the nutritional status, the sample group's weight and height were obtained. Results revealed that the children from the private institution showed significant differences in psychomotor development compared to the results of the children from the municipal institution. Furthermore, the overweight or obese individuals, classified as suffering from malnutrition due to excessive amounts of food, showed inferior motor perfor-mance compared to the children with normal or below average weight. With regards to body image, the higher the weight, the lower the child's own body image.

[VARIATION ON SOMATOTYPE AND WAIST CIRCUMFERENCE IN A SAMPLE OF UNIVERSITY STUDENTS BETWEEN YEARS 2012 AND 2014 IN THE TEMUCO, CHILE]. / CARACTERIZACIÓN DEL SOMATOTIPO Y CIRCUNFERENCIA DE CINTURA EN UNA MUESTRA DE ESTUDIANTES UNIVERSITARIOS ENTRE LOS AÑOS 2012 -2014 DE LA CIUDAD DE TEMUCO, CHILE.

UNLABELLED: Objetive: to determine variation on somatotype and waist circumference (WC) in university students during a three-year follow-up. METHODS: a total sample of 36 first-year university students was recruited in 2012. Heath-Carter anthropometric somatotype and waist circumference were assessed in 2012 and 2014. RESULTS: male students showed mesomorph component (3.7-5.3-1.8) as dominant in 2012, while in 2014 endomorph and mesomorph components were equal (4.1-4.5-1.6). The somatotype in female students was dominated by endomorphic component in 2012 (5.4-4.6-1.7) and 2014 (5.3-3.2-1.6). Among somatotypes, for both, male and female students, only endomorphy showed statistically significant differences during the follow-up (p = 0,00). The endo-mesomorphic somatotype was dominant in men with 61.9% in 2012 and 38.1% in 2014. Women showed a higher percentage for meso-endomorphic somatotype with 60% in 2012 and 80% in 2014. In men, WC between 2014 and 2014 showed a statistically significant increment of 4.8% (p = 0,00). In women WC increased by 13.3% but no statistically difference was observed (p = 0,10). CONCLUSIONS: this study showed somatotypic change with tendency in increased endomorphy in both men and women. Despite WC values were similar to the national mean, cases with cardiometabolic risk increased during the follow-up period based on this measurement.

Objetivo: determinar la variación del somatotipo y la circunferencia de la cintura (CC) en estudiantes universitarios en un periodo de tres años. Método: se seleccionó una muestra de 36 estudiantes de primer año en 2012, en los cuales se evaluó el somatotipo de Heath-Carter y la circunferencia de cintura en los años 2012 y 2014. Resultados: en los hombres, el componente dominante en el año 2012 fue la mesomorfia (3,7-5,3-1,8), mientras que en el año 2014 la endomorfia y la mesomorfia son iguales (4,1-4,5-1,6). En las mujeres, en el año 2012 (5,4- 4,6-1,7) y en el año 2014 (5,3-3,2-1,6) dominó la endomorfia. De los componentes del somatotipo, en ambos sexos, la endomorfia es el único que presenta diferencias estadísticamente significativas entre los años estudiados (p = 0,00) El somatotipo dominante en los hombres fue del tipo endo-mesomorfo, con un 61,9% en el año 2012 y con un 38,1% en el 2014. En las mujeres, el somatotipo meso-endomorfo es el que presenta mayor porcentaje en el año 2012, con un 60%, y en el año 2014, con un 80%. La CC en los hombres entre el año 2012 y 2014 tuvo un aumento estadísticamente significativo de 4,8% (p = 0,00). En las mujeres, el aumento presentado de 13,3% no fue estadísticamente significativo (p = 0,10). Conclusiones: en este estudio se observó una modificación del somatotipo con una tendencia al aumento de la endomorfia en ambos sexos. Con respecto a la CC, si bien los valores son cercanos a la media nacional, entre ambos años aumentaron los casos que presentaban riesgo cardiometabólico de acuerdo a esta medición.

Caracterización del somatotipo y circunferencia de cintura en una muestra de estudiantes universitarios entre los años 2012 -2014 de la ciudad de Temuco, Chile / Variation on somatotype and waist circumference in a sample of university students between years 2012 and 2014 in the Temuco, Chile

Objetivo: determinar la variación del somatotipo y la circunferencia de la cintura (CC) en estudiantes universitarios en un periodo de tres años. Método: se seleccionó una muestra de 36 estudiantes de primer año en 2012, en los cuales se evaluó el somatotipo de Heath-Carter y la circunferencia de cintura en los años 2012 y 2014. Resultados: en los hombres, el componente dominante en el año 2012 fue la mesomorfia (3,7-5,3-1,8), mientras que en el año 2014 la endomorfia y la mesomorfia son iguales (4,1-4,5-1,6). En las mujeres, en el año 2012 (5,4- 4,6-1,7) y en el año 2014 (5,3-3,2-1,6) dominó la endomorfia. De los componentes del somatotipo, en ambos sexos, la endomorfia es el único que presenta diferencias estadísticamente significativas entre los años estudiados (p=0,00) El somatotipo dominante en los hombres fue del tipo endo-mesomorfo, con un 61,9% en el año 2012 y con un 38,1% en el 2014. En las mujeres, el somatotipo meso-endomorfo es el que presenta mayor porcentaje en el año 2012, con un 60%, y en el año 2014, con un 80%. La CC en los hombres entre el año 2012 y 2014 tuvo un aumento estadísticamente significativo de 4,8% (p=0,00). En las mujeres, el aumento presentado de 13,3% no fue estadísticamente significativo (p=0,10). Conclusiones: en este estudio se observó una modificación del somatotipo con una tendencia al aumento de la endomorfia en ambos sexos. Con respecto a la CC, si bien los valores son cercanos a la media nacional, entre ambos años aumentaron los casos que presentaban riesgo cardiometabólico de acuerdo a esta medición (AU)

Objetive: to determine variation on somatotype and waist circumference (WC) in university students during a three-year follow-up. Methods: a total sample of 36 first-year university students was recruited in 2012. Heath-Carter anthropometric somatotype and waist circumference were assessed in 2012 and 2014. Results: male students showed mesomorph component (3.7-5.3-1.8) as dominant in 2012, while in 2014 endomorph and mesomorph components were equal (4.1-4.5-1.6). The somatotype in female students was dominated by endomorphic component in 2012 (5.4-4.6-1.7) and 2014 (5.3-3.2-1.6). Among somatotypes, for both, male and female students, only endomorphy showed statistically significant differences during the follow-up (p=0,00). The endo-mesomorphic somatotype was dominant in men with 61.9% in 2012 and 38.1% in 2014. Women showed a higher percentage for meso-endomorphic somatotype with 60% in 2012 and 80% in 2014. In men, WC between 2014 and 2014 showed a statistically significant increment of 4.8% (p=0,00). In women WC increased by 13.3% but no statistically difference was observed (p=0,10). Conclusions: this study showed somatotypic change with tendency in increased endomorphy in both men and women. Despite WC values were similar to the national mean, cases with cardiometabolic risk increased during the follow-up period based on this measurement (AU)

Involvement of leucocyte/endothelial cell interactions in anorexia nervosa.

BACKGROUND: Anorexia nervosa is a common psychiatric disorder in adolescence and is related to cardiovascular complications. Our aim was to study the effect of anorexia nervosa on metabolic parameters, leucocyte-endothelium interactions, adhesion molecules and proinflammatory cytokines. MATERIALS AND METHODS: This multicentre, cross-sectional, case-control study employed a population of 24 anorexic female patients and 36 controls. We evaluated anthropometric and metabolic parameters, interactions between leucocytes polymorphonuclear neutrophils (PMN) and human umbilical vein endothelial cells (HUVEC), proinflammatory cytokines such as tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) and soluble cellular adhesion molecules (CAMs) including E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). RESULTS: Anorexia nervosa was related to a decrease in weight, body mass index, waist circumference, systolic blood pressure, glucose, insulin and HOMA-IR, and an increase in HDL cholesterol. These effects disappeared after adjusting for BMI. Anorexia nervosa induced a decrease in PMN rolling velocity and an increase in PMN rolling flux and PMN adhesion. Increases in IL-6 and TNF-α and adhesion molecule VCAM-1 were also observed. CONCLUSIONS: This study supports the hypothesis of an association between anorexia nervosa, inflammation and the induction of leucocyte-endothelium interactions. These findings may explain, in part at least, the increased risk of vascular disease among patients with anorexia nervosa.

Altered mitochondrial function and oxidative stress in leukocytes of anorexia nervosa patients.

CONTEXT: Anorexia nervosa is a common illness among adolescents and is characterised by oxidative stress. OBJECTIVE: The effects of anorexia on mitochondrial function and redox state in leukocytes from anorexic subjects were evaluated. DESIGN AND SETTING: A multi-centre, cross-sectional case-control study was performed. PATIENTS: Our study population consisted of 20 anorexic patients and 20 age-matched controls, all of which were Caucasian women. MAIN OUTCOME MEASURES: Anthropometric and metabolic parameters were evaluated in the study population. To assess whether anorexia nervosa affects mitochondrial function and redox state in leukocytes of anorexic patients, we measured mitochondrial oxygen consumption, membrane potential, reactive oxygen species production, glutathione levels, mitochondrial mass, and complex I and III activity in polymorphonuclear cells. RESULTS: Mitochondrial function was impaired in the leukocytes of the anorexic patients. This was evident in a decrease in mitochondrial O2 consumption (P<0.05), mitochondrial membrane potential (P<0.01) and GSH levels (P<0.05), and an increase in ROS production (P<0.05) with respect to control subjects. Furthermore, a reduction of mitochondrial mass was detected in leukocytes of the anorexic patients (P<0.05), while the activity of mitochondrial complex I (P<0.001), but not that of complex III, was found to be inhibited in the same population. CONCLUSIONS: Oxidative stress is produced in the leukocytes of anorexic patients and is closely related to mitochondrial dysfunction. Our results lead us to propose that the oxidative stress that occurs in anorexia takes place at mitochondrial complex I. Future research concerning mitochondrial dysfunction and oxidative stress should aim to determine the physiological mechanism involved in this effect and the physiological impact of anorexia.